淫羊藿苷与淫羊藿次苷Ⅱ的体外抗氧化作用

J4 ›› 2012, Vol. 38 ›› Issue (3): 423-428.

淫羊藿苷与淫羊藿次苷Ⅱ的体外抗氧化作用

（1.陕西师范大学物理学与信息技术学院生物医学工程实验室|陕西　西安 710062；2.陕西师范大学生命科学学院生物化学与分子生物学实验室|陕西　西安 710062）

收稿日期:2012-02-21 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 孙润广 E-mail:(Tel:029-85308141,E-mail: sunrunguang@snnu.edu.cn)
作者简介:包宇(1982-)|女|吉林省临江市人|在读理学博士|主要从事天然产物的分离鉴定和与功能评价研究。
基金资助:
国家自然科学基金资助课题(10874108)

Antioxidant effects |of icariin and icariside Ⅱ in vitro

包宇¹|杨建雄²,孙润广¹

(1.Biomedical Engineering Laboratory|School of Physics and Information Technology|Shaanxi Normal University|Xi’an 710062|China；2.Biochemistry and Molecular Biology Laboratory,School of Life Science|Shaanxi Normal University|Xi’an 710062|China)

Received:2012-02-21 Online:2012-05-28 Published:2012-05-28

摘要/Abstract

摘要：

的：检测淫羊藿苷和淫羊藿次苷Ⅱ的体外抗氧化活性，阐明淫羊藿黄酮类化合物的抗氧化机制。方法: 以抗坏血酸(Vc)、二丁基羟基甲苯(BHT)为阳性对照，测定淫羊藿苷、淫羊藿次苷Ⅱ和BHT对1，1-二苯基-2-苦苯肼自由基(DPPH?)的清除率；铁氰化钾还原法测定其还原力；利用NADH-NBT-PMS系统测定其对超氧阴离子(O2－?)的清除率；2-脱氧-D-核糖降解法测定淫羊藿苷和淫羊藿次苷Ⅱ对羟自由基(OH?)的清除率，硫代巴比妥酸法测定其对脂质过氧化的抑制率，β-胡萝卜素-亚油酸自氧化体系测定其总抗氧化能力。结果:不同样品对DPPH?均有一定的清除作用，淫羊藿次苷Ⅱ清除DPPH?的能力较淫羊藿苷强(P<0.05)。淫羊藿苷和淫羊藿次苷Ⅱ在浓度梯度范围内对O2－?有一定的清除作用，均表现出一定的浓度依赖性，与同浓度的BHT比较，淫羊藿苷和淫羊藿次苷Ⅱ对O2－?的清除率较低，且淫羊藿苷的清除能力略低于淫羊藿次苷Ⅱ。淫羊藿苷和淫羊藿次苷Ⅱ在浓度为0.1～0.5 g.L^-1时对OH?清除率分别为(16.76±0.35)%~(40.56±1.46)%和(15.65±0.72)%~(28.51±0.91)%。当浓度为0.9 g.L^-1时，淫羊藿苷、Vc和淫羊藿次苷Ⅱ对脂质过氧化的抑制率为(58.79±1.56) %、(75.05±2.12)%和 (37.82±1.43)%。随着浓度的增加，抑制率不再有显著的增加。随着样品浓度的增加，淫羊藿苷、淫羊藿次苷Ⅱ和标准品Vc还原力均表现出浓度依赖性。30~120 min内淫羊藿次苷Ⅱ的抗氧化活性均低于淫羊藿苷和BHT（P<0.05或P<0.01）。淫羊藿苷和淫羊藿次苷Ⅱ在体外抗氧化的各项指标中表现出的抗氧化活性均弱于Vc和BHT。结论: 淫羊藿苷和淫羊藿次苷Ⅱ在清除DPPH?、O2－?、OH?和抑制脂质过氧化、总抗氧化能力和还原力方面，均具有明显的抗氧化能力。

关键词: 淫羊藿苷；淫羊藿次苷Ⅱ；自由基；脂质过氧化

Abstract:

To investigate the antioxidant activities of icariin and icariside Ⅱ,and to clarify the possible antioxidant mechanism.Methods Using Vc and butylated hydroxytoluene (BHT)as positive controls,the clearance rates of icariin ,icariside Ⅱ and BHT on 1，1-diphenyl-2-picryl-hydrazyl radical (DPPH?),superoxide anion(O２－?) and hydroxyl free radical (OH?) were measured;the reduction rates of samples were detected by the system of potassium ferricyanide;the capacities of samples were detected by NADH-NBT-PMS system; the inhibitory rates of lipid peroxidation were detected by thiobarbituric acid (TBA) method;the total antioxidant activities were determined by bleaching of β-carotene suboleic acid system.Results The samples showed concentration-dependent scavenging capacities of DPPH?.The clearance abilities of icariside Ⅱ were higher than those of icariin (P<0.05).The clearance effects of icariin and icariside Ⅱ were concentraion-dependent;compared with the same concentration of BHT,the O2－? clearance rates of icariside Ⅱ and icariin were slightly lower, and the clearance rate of icariin was much lower than that of icariside Ⅱ.The OH? clearance rates of icariin and icariside Ⅱ in the concentration of 0.1-0.5 g.L^-1 were (16.76±0.35)% -(40.56±1.46)% and (15.65±0.72)% -(28.51±0.91)%. When the concentration was 0.9 g.L^-1,the inhibitory rates of lipid peroxidation of icariin,Vc and icariside Ⅱ were (58.79±1.56)%,(75.05±2.12)% and (37.82±1.43)%.The inhibitory rates no longer changed with the dose increasing.As the sample concentration increasing,the reduction capacities of icariin,Vc and icariside Ⅱ showed concentration-dependent tendence.In 30-120 min the total antioxidant activity of icariside Ⅱ was lower than those of icariin and BHT(P<0.05 or P<0.01).The antioxidant activities of icariin and icariside Ⅱ in vitro were weaker than those of Vc and BHT.Conclusion Icariin and icariside Ⅱ shows obvious antioxidant activities in scavenging of DPPH?,O2-? and OH? and inhibition of lipid peroxidation, the total antioxidant activity and the reduction activity.

Key words: icariin, icariside Ⅱ, free radical, lipid peroxidation

中图分类号:

R943.3

包宇|杨建雄,孙润广. 淫羊藿苷与淫羊藿次苷Ⅱ的体外抗氧化作用[J]. J4, 2012, 38(3): 423-428.