基质金属蛋白酶9基因多态性与冠心病的关联性

J4 ›› 2012, Vol. 38 ›› Issue (4): 755-760.

基质金属蛋白酶9基因多态性与冠心病的关联性

刘成斌|吴海帝|李晶|徐国良|秦玲

吉林大学第一医院二部心内科|吉林长春 130031

收稿日期:2012-03-14 出版日期:2012-07-28 发布日期:2012-07-16
通讯作者: 秦玲(Tel:0431-84808229, E-mail:qinling1958 ＠yahoo.com.cn); 徐国良(Tel:0431-84808227, E-mail:glxublog@yahoo.cn) E-mail:E-mail:qinling1958 ＠yahoo.com.cn;E-mail:glxublog@yahoo.cn
作者简介:刘成斌(1986-)|男|山东省莱州市人, 医学硕士, 主要从事冠心病的临床研究。
基金资助:
吉林省科技厅科研基金资助课题（200905138）

Association |between matrix metalloproteomase-9 polymorphism and |coronary heart disease

LIU Cheng-bin,WU Hai-di,LI Jing,XU Guo-liang,QIN Ling

Department of Cardiology, First Hospital, Jilin University, Changchun 130031,
China

Received:2012-03-14 Online:2012-07-28 Published:2012-07-16

摘要/Abstract

摘要：

目的：探讨基质金属蛋白酶9（MMP-9）基因C1562T、R279Q单核苷酸多态性(SNPs)与冠状动脉粥样硬化性心脏
病(CHD)的相关性。方法：258例CHD患者和183例健康体检者作为研究对象，采用酶联免疫吸附试验（ELISA）测定血清MMP-9水平，应用聚合酶链式反应-限制性片段长度多态（PCR-RFLP）方法对MMP-9基因C1562T和R279Q多态位点进行SNPs基因型检测，运用统计学软件SPSS 17.0分析等位基因和基因型频数分布。结果：CHD组患者血清MMP-9水平明显高于健康对照组（P<0.05）。CHD 组MMP-9基因C1562T多态位点CT+TT基因型和T等位基因频数（24.4%和11.8%）明显高于健康对照组（13.8%和7.3%）（P<0.05）。CHD组MMP-9基因R279Q多态位点G+GG基因型和G等位基因频数（60.2%和41.7%）与健康对照组（68.0%和41.5%）比较差异无统计学意义（P>0.05）。结论：CHD患者伴有MMP-9　的过度释放，MMP-9基因C1562T多态位点与CHD发病有关联，R279Q多态位点与
CHD发病无关联。

关键词: 冠状动脉粥样硬化性心脏病；基质金属蛋白酶9；基因多态性

Abstract:

Objective To investigate the association between the single polymorphisms(SNPs) of MMP-9 gene C1562T,R279Q and coronary heart disease (CHD). Methods 258 patients diagnosed with CHD and 183 healthy contols were selected. The serum levels of MMP-9 were determinated using enzyme-linked immunosorbent assay (ELISA). PCR-RFLP analysis was applied to detect the C1562T and R279Q SNPs of MMP-9 gene. Statistic
al soft ware SPSS 17.0 was used to analyze the frequency distribution of alleles and genotypes. Results The serum MMP-9 level in CHD group was significantly higher than that in control group（P<0.05）. The C1562T genotype frequencies of CT+TT genotypes and T allele were 24.4% and 11.8% in CHD group and 13.8% and 7.3% in control group, respectively, and there were significant differences between two groups (P<0.05). The R279Q genotype frequencies of AG+GG genotypes and G allele were 60.2% and 41.7% in CHD group and 68.0% and 41.5% in control group, respectively, and there were no significant differences between two groups(
P>0.05). Conclusion The MMP-9 level in patients with CHD is increased and the polymorphisms in MMP-9 gene C1562T associates with the susceptibility to the CHD,but there is no association between R279Q polymorphism and CHD.

Key words: coronary heart disease;marix metalloproteinase-9;gene polymorphism

中图分类号:

R543.3

刘成斌|吴海帝|李晶|徐国良|秦玲. 基质金属蛋白酶9基因多态性与冠心病的关联性[J]. J4, 2012, 38(4): 755-760.

LIU Cheng-bin,WU Hai-di,LI Jing,XU Guo-liang,QIN Ling. Association |between matrix metalloproteomase-9 polymorphism and |coronary heart disease[J]. J4, 2012, 38(4): 755-760.