眼附属器淋巴增生性疾病的衣原体免疫荧光检测及其意义

J4 ›› 2012, Vol. 38 ›› Issue (4): 796-799.

眼附属器淋巴增生性疾病的衣原体免疫荧光检测及其意义

杨立军¹|肖利华²|黑砚²|康莉²

1.辽宁医学院北京武警总医院研究生培养基地|北京 100039；2.北京武警总医院眼眶病研究所|北京100039

收稿日期:2012-05-18 出版日期:2012-07-28 发布日期:2012-07-28
通讯作者: 肖利华( Tel: 010 -88276686,E-mail: xiaolihuawj@sina.com) E-mail:xiaolihuawj@sina.com
作者简介:杨立军(1980-)|男|吉林省长岭县人|在读医学硕士|主要从事眼眶病学临床研究。
基金资助:
武警总医院一类资助课题（WZ2011001）

Detection of Chlamydia immunofluorescence of ocular adnexal |lymphoproliferative diseases

YANG Li-jun¹|XIAO Li-hua²|HEI Yan²|KANG Li²

1.Postgraduate Training Base|General Hospital of Armed Police Forces|Liaoning Medical University|Beijing 100039|China；2.Institute of Orbital Disease|General Hospital of Armed Police Forces|Beijing 100039|China

Received:2012-05-18 Online:2012-07-28 Published:2012-07-28

摘要/Abstract

摘要：

目的：检测眼附属器淋巴增生性疾病中衣原体感染情况，探讨眼附属器淋巴增生性疾病与衣原体感染的关联性。方法：选取眼附属器淋巴增生性疾病患者118例为实验组，其中胃黏膜相关淋巴组织(MALT)淋巴瘤46例，淋巴组织反应性增生41例，其他类型淋巴瘤31例；另选取眼附属器非淋巴增生性疾病标本20例作为对照组，其中包括脉管瘤17例，甲状腺相关眼病3例。采用免疫荧光的方法进行衣原体检测，在每个高倍镜视野(×1 000）下出现10以上包涵体则为阳性，反之为阴性。结果：118例眼附属器淋巴增生性疾病患者中80例衣原体感染阳性（67.79%），对照组眼附属器非淋巴增生性疾病20例中无阳性病例，组间比较差异有统计学意义（χ2= 32.262，P<0.001）。MALT淋巴瘤衣原体感染阳率与非MALT淋巴瘤组比较差异无统计学意义(P>0.05)。结论：常见眼附属器淋巴增生性疾病与衣原体感染可能存在关联性，尤其MALT淋巴瘤和淋巴组织反应性增生衣原体感染率较高。衣原体感染的检测与治疗为眼附属器淋巴增生性疾病诊治提供了新的方向。

关键词: 眼附属器淋巴增生性疾病；衣原体；免疫荧光；胃黏膜相关淋巴组织淋巴瘤

Abstract:

Abstract:Objective To detect the rate of Chlamydia infection of ocular adnexal lymphoproliferative diseases and to investigate the relativity between ocular adnexal lymphoproliferative diseases and   Chlamydial infection.
Methods 118　patients with ocular adnexal lymphoproliferative diseases were selected as experiment group, and 46 cases among them with mucosa associated lymphoid tissue(MALT) lymphoma,41 cases of hyperplasia of reactive lymphoid tissue,31 cases of other types of lymphoma. 20 cases of samples with ocular adnexal non- lymphoproliferative
diseases were selected as    control group,including 17 cases of vascular tumor and 3 cases of thyroid associated ophthalmopathy.Then the method of immunofluorescence was used to detect the Chlamydia in two groups.It was positive when it showed over ten inclusionbodies in the vision of a high-rate magnification(×1 000).Otherwise it was negative.
Results 80 cases of ocular adnexal lymphoproliferative diseases in 118 cases had positive chlamydia infection(67.79%),while there was0 case among 20 cases of ocular adnexal non- lymphoproliferative diseases in control group(χ2=32.262, P<0.001).There was no significant differecnce of pasitive rate of Chlamydia infection between MALT lymphoma patients and non-MALT lymphoma patients(P>0.05). Conclusion There might be a relationship between common ocular adnexal lymphoproliferative diseases and    Chlamydia infection,especially the MALT lymphoma and the hyperplasia of reactive lymphoid tissue  have higher rates of   Chlamydia infection.The detection and treatment of Chlamydia infection provide a new direction of research for the diagnosis and treatment of ocular adnexal lymphoproliferative diseases.

Key words: ocular adnexal , lymphoproliferative diseases;Chlamydia; , immunofluorescence, mucosa associated lymphoid tissue lymphoma

中图分类号:

R777

杨立军|肖利华|黑砚|康莉. 眼附属器淋巴增生性疾病的衣原体免疫荧光检测及其意义[J]. J4, 2012, 38(4): 796-799.

YANG Li-jun|XIAO Li-hua|HEI Yan|KANG Li. Detection of Chlamydia immunofluorescence of ocular adnexal |lymphoproliferative diseases[J]. J4, 2012, 38(4): 796-799.