J4 ›› 2012, Vol. 38 ›› Issue (5): 836-840.

• 基础研究 • 上一篇    下一篇

雌孕激素对ALI大鼠Ⅱ型肺泡上皮细胞ENaC表达的影响

罗玲|王导新   

  1. (重庆医科大学附属第二医院呼吸内科|重庆400010)
  • 收稿日期:2012-06-14 出版日期:2012-09-28 发布日期:2012-09-28
  • 通讯作者: 王导新 E-mail:(Tel:023-63693093,E-mail:wangdaoxin1@163.com)
  • 作者简介:罗 玲(1978-),女,重庆市人,主治医师,医学博士,主要从事ARDS的机制及治疗研究。
  • 基金资助:

    国家自然科学基金资助课题(30971303)

Influence of estradiol and progestogen in type Ⅱ alveolar ENaC in rats with acute lung injury

LUO Ling,WANG Dao-xin   


  1. (Department of Respiratory Medicine, Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China)
  • Received:2012-06-14 Online:2012-09-28 Published:2012-09-28

摘要:

目的:研究雌孕激素对急性肺损伤(ALI)大鼠Ⅱ型肺泡上皮细胞钠通道(ENaC)表达的影响,探讨雌孕激素促进肺泡内液体清除的机制。方法:雌性未成年SD大鼠40只,随机分为正常对照组、ALI模型组、雌孕激素联合治疗A组和雌孕激素联合治疗B组,正常对照组颈静脉注入生理盐水(8 mL?kg-1),后3组经颈静脉注入油酸(0.1 mL?kg-1)建立ALI模型,其中雌孕激素联合A、B组分别在油酸注入前12和36 h皮下注射不同比例的雌孕激素混合液0.1 mL(A组 1∶1 000,B组 1∶200)。建模后6 h处死大鼠,测定肺湿重/干重比值,计算肺系数,观察肺组织病理改变,测定肺组织匀浆中α-ENaC、β-ENaC和γ-ENaC mRNA及蛋白的表达水平。结果:①与正常对照组比较,ALI模型组肺湿重/干重比值及肺系数增加,差异有统计学意义(P<0.01);与ALI模型组比较,雌孕激素联合治疗组肺湿重/干重比值及肺系数下降,以A组明显,差异有统计学意义(P<0.05)。② ALI模型组大鼠出现明显肺水肿、出血及炎性细胞浸润;与模型组比较,联合治疗组大鼠肺水肿、出血及炎性细胞浸润较轻,A组稍明显。③与正常对照组比较,ALI组α-ENaC和γ-ENaC mRNA及蛋白表达水平明显降低,差异有统计学意义(P<0.01);与ALI模型组比较,联合治疗组α-ENaC和γ-ENaC mRNA及蛋白表达水平有增加,以A组增加明显,差异有统计学意义(P<0.05)。结论:雌孕激素可以通过增加ALI大鼠Ⅱ型肺泡上皮细胞α-ENaC和γ-ENaC mRNA及蛋白的表达水平,促进肺泡内液体的清除。

关键词: 雌激素;孕激素;钠通道;急性肺损伤;急性呼吸窘迫综合征

Abstract:

Abstract:Objective
To study the influence of 17β-estradiol and progestogen in type Ⅱalveolar epithelial Na+ channel(ENaC)in rats with acute lung injury(ALI) and explore the mechanism of estradiol and progestogen in  promoting the  clearance of alveolar fluid.Methods 40 immature female SD rats were randomly assigned into 4 groups: normal control group(injected with 8 mL?kg-1 saline via venous),ALI model group,17β-estradiol and  progestogen  combination A and B group.The rats in last three group were injected with 0.1  mL?kg-1 oleic acid via venous to establish ALI model.The rats in combination A and B groups  were injected subcutaneously with 17β-estradiol and  progestogen mixed liquor 0.1 mL in different ratios (A group 1∶1 000,B group 1∶200) at 12 and 36 h before oleic acid injection.All the rats were sacrificed at 6 h after oleic acid injection.The wet/dry ratio and the lung coefficient were determined, the pathological changes of  lung  tissues  were  observed, and the expressions of α-ENaC,β-ENaC and γ-ENaC mRNA and protein were determined.Results
① Compared with normal control groups,the wet/dry ratio and the lung coefficient in ALI model group were increased significantly (P<0.01);in combination A and B groups, the wet/dry ratios and the lung coefficients were decreased compared with model group,especially in  A group (P<0.05).②Obvious pulmonary edema,bleeding and inflammatory cell infiltration were observed in ALI group.However, the extent of  pulmonary edema,bleeding and inflammatory cell infiltration were decreased markedly in combination  A and B groups, especially in  A groups.③ Compared with normal control group,the  expressions of α-ENaC and γ-ENaC mRNA and protein were decreased in ALI model group(P<0.01); compared with ALI group,they were increased significantly in combination A and B groups,especially in A  group(P<0.05).Conclusion 17β-estradiol and progestogen can increase the expressions of  type Ⅱ alveolar epithelial α-ENaC and γ-ENaC mRNA and protein in rats with ALI,and promote the clearance of alveolar fluid.

Key words: estrogen; progestogen;sodium channel;acute lung injury;acute respiratory distress syndrome

中图分类号: 

  • R363