中国汉族肢端恶性黑素瘤的差异蛋白质组学研究

J4 ›› 2012, Vol. 38 ›› Issue (5): 866-869.

中国汉族肢端恶性黑素瘤的差异蛋白质组学研究

姜兰香¹| 庞传超¹| 巫毅¹| 徐春阳¹| 夏建新¹| 王延龙¹| 张磊²|桑海燕³

（1.吉林大学第二医院皮肤科|吉林长春 130041；2.吉林大学第二医院儿科|吉林长春 130041;3.吉林大学中日联谊医院心内科|吉林长春 130033）

收稿日期:2012-06-04 出版日期:2012-09-28 发布日期:2012-09-28
通讯作者: 张磊 E-mail:(Tel: 0431-88796802，E-mail: zhang_lei@jlu.edu.cn)
作者简介:姜兰香（1964-）|女|吉林省农安县人|副教授|医学硕士|主要从事皮肤性病的临床研究。
基金资助:
吉林省科技厅科研基金资助课题 (20090475)

Differential proteomic analysis of malignant melanoma in Chinese Han population

JIANG Lan-xiang¹,PANG Chuan-chao¹,WU Yi¹,XU Chun-yang¹,XIA Jian-xin¹,WANG Yan-long¹,ZHANG Lei²,SANG Hai-yan³

(1. Department of Dermatology,Second Hospital,Jilin University,Changchun 130041 China|2. Department of Pediatrics,Second Hospital,Jilin University,Changchun 130041,China；3.Department of Cardiology,China-Japan Union Hospital,Jilin University,Changchun 130033,China)

Received:2012-06-04 Online:2012-09-28 Published:2012-09-28

摘要/Abstract

摘要：

目的：研究恶性黑素瘤与正常皮肤组织的蛋白质表达差异，寻找可能的恶性黑素瘤的诊断标志蛋白和治疗靶点。方法：选取经病理确诊的3例恶性黑素瘤切除标本，提取样本的总蛋白质，运用差异凝胶电泳技术分离蛋白质，DeCyder软件分析蛋白图谱的差异，以基质-辅助激光解吸/电离飞行时间串联质谱(MALDI-TOF/TOF-MS)对差异表达蛋白质进行质谱鉴定。结果：质谱鉴定出差异蛋白质点12个，按功能分为4类，其中上调蛋白质3类，为14-3-3蛋白sigma、角蛋白和serpin B3；下调蛋白质1类，为白蛋白。结论：14-3-3蛋白sigma和serpin B3可能参与恶性黑素瘤的发生和发展，其差异表达提示了可能的恶性黑素瘤的发病机制。

关键词: 恶性黑素瘤；蛋白质组；基质辅助激光解吸电离飞行时间串联质谱

Abstract:

Abstract:Objective
To study the proteomic differences between malignant melanoma tissue and normal skin tissue and to look for the possible marker for diagnosis and therapy target of malignant melanoma. Methods Three samples of malignant melanoma tissues were ascertained by pathologic test. The total protein was extracted,separated with the method of 2D-DIGE; the difference of the proteomics was analyzed with DeCyder software; the differential-expressed protein spots were identified with MALDI-TOF/TOF-MS．Results A total of 12 protein spots were identified and categorized into 4 classes according to the function including 3 kinds of up-regulated proteins (14-3-3 protein sigma,keratin and serpin B3)，and 1 kind of down-regulated protein (album)．Conclusion 14-3-3 protein sigma and serpin B3 are possible involved in the occurance and developmentof malignant melanoma. The mechanism of album change in the tissue of malignant melanoma remains unclear. The change of those four proteins suggests the possible pathogenesis of malignant melanoma.

Key words: malignant melanoma, proteomics;matrix-assisted laser desorption/ionization-time of flight-time of flight-mass spectrometry

中图分类号:

R-751

姜兰香|庞传超| 巫毅| 徐春阳| 夏建新| 王延龙| 张磊|桑海燕. 中国汉族肢端恶性黑素瘤的差异蛋白质组学研究[J]. J4, 2012, 38(5): 866-869.

JIANG Lan-xiang,PANG Chuan-chao,WU Yi,XU Chun-yang,XIA Jian-xin,WANG Yan-long. Differential proteomic analysis of malignant melanoma in Chinese Han population[J]. J4, 2012, 38(5): 866-869.