J4 ›› 2012, Vol. 38 ›› Issue (6): 1106-1109.

• 基础研究 • 上一篇    下一篇

抑制ClC-3表达对顺铂诱导人卵巢癌SKOV3/DDP细胞凋亡的促进作用

于春艳1|韩命龙2|钟加滕3|孙连坤3|刘玉和1   

  1. 1.北华大学基础医学院病理学教研室|吉林 吉林132013;2.吉林市公安局昌邑分局法医室|吉林 吉林132001;3.吉林大学白求恩医学院病理生理学教研室|吉林 长春 130021)
  • 收稿日期:2012-06-19 发布日期:2012-11-28
  • 通讯作者: 刘玉和(Tel:0432-64608556,E-mail: bhlyh1959@163.com) E-mail:bhlyh1959@163.com
  • 作者简介:于春艳(1975-),女,吉林省吉林市人|副教授|医学博士|主要从事肿瘤病理 学方面的研究。
  • 基金资助:

    吉林省教育厅资助课题(吉教科合字2009第156号,吉教科合字2011第117号,吉教科合字2012第394号吉林省科技厅自然科学基金资助课题(201215103)

Promoting effect of inhibiting |ClC-3 expression on apoptosis induced by cisplatin in human ovarian cancer SKOV3/DDP cells

YU Chun-yan1,HAN Ming-long 2,ZHONG Jia-teng3,SUN Lian-kun3,LIU Yu-he 1   

  1. 1.Department of Pathology,College of Basic Medicine,Beihua University,Jinlin 132013,China; 2. Department of Firensic Medicine,Jilin Public Security Bureau Changyi Branch,Jinlin 132001,China|3. Department of Pathophysiology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China
  • Received:2012-06-19 Published:2012-11-28

摘要:

目的:探讨抑制氯离子通道-3(ClC-3)基因表达对顺铂(DDP)诱导的人卵巢癌SKOV3/DDP细胞凋亡的促进作用,为卵巢癌的治疗提供实验依据。方法:转染pSH1-siRNA-ClC-3 重组质粒至人卵巢癌SKOV3/DDP细胞,Western blotting方法检测转染重组质粒对ClC-3蛋白表达的影响;MTT法检测细胞增殖率;Western blotting方法检测凋亡相关蛋白细胞色素C(Cyt-c)及Caspase-3活化片段蛋白表达。结果:与SKOV3细胞比较,SKOV3/ DDP细胞中ClC-3蛋白表达水平增加( P<0.05);转染pSH1-siRNA-ClC-3 重组质粒后,SKOV3/DDP细胞ClC蛋白表达水平明显降低;ClC-3-siRNA联合DDP作用后,SKOV3/DDP细胞增殖率下降( P<0.05),Cyt-c和Caspase-3活化片段蛋白表达水平增加( P<0.05) 。结论:pSH1-siRNA-ClC-3可有效抑制SKOV3/DDP细胞ClC-3蛋白的表达,并促进DDP诱导SKOV3/DDP细胞凋亡。

关键词:  卵巢肿瘤;氯通道-3;顺铂;细胞凋亡

Abstract:

Objective To observe the promoting effect of inhibiting chloride channel-3(ClC-3) gene expression on the apoptosis of human ovarian cancer SKOV3/DDP cells induced by cisplatin (DDP),and to provide the experimental basis for   treatment of ovarian cancer.Methods The recombinant plamid pSH1-siRNA-ClC-3 was transfected into SKOV3/DDP cells.The ClC-3 expression changes in SKOV3/DDP cells transfected with pSH1-SiRNA-ClC-3 was detected by Western blotting.The vitality of SKOV3/DDP cells was measured by MTT assay.The expression levels of apoptosis-related proteins cytochrome C (Cyt-C) and Cleaved Caspase-3 were determined byWestern blotting. Results  Compared with SKOV3 cells,the ClC-3 expression levels  in SKOV3/DDP cells was increased (P<0.05).Transfection of pSH1-SiRNA-ClC-3 suppressed the expression of ClC-3 efficiently in SKOV3/DDP cells (P<0.05).The proliferation rate of SKOV3/DDP cells was  decreased (P<0.05),and the cyt-C and Cleaved Caspase-3 expression were increased  after treated with ClC-3-siRNA combined with  DDP(P<0.05).Conclusion pSH1-SiRNA-ClC-3 could suppress the expression of ClC-3 efficiently and enhance the apoptosis induced by DDP in SKOV3/DDP cells.

Key words:  ovarian tumor, chloride channel-3;cisplatin;apoptosis

中图分类号: 

  • R737.31