绞股蓝总皂苷对光老化HaCaT细胞中p38MAPK蛋白和c-Jun mRNA表达的抑制作用

J4 ›› 2012, Vol. 38 ›› Issue (6): 1147-1151.

• 基础研究 • 上一篇下一篇

绞股蓝总皂苷对光老化HaCaT细胞中p38MAPK蛋白和c-Jun mRNA表达的抑制作用

陶冶¹,张小卿²,吴琼³,吴景东⁴

1.辽宁中医药大学基础医学院中医基础教研室,辽宁沈阳110847；2.辽宁中医药大学针灸推拿学院针灸教研室,辽宁沈阳110847；3.中国医科大学附属盛京医院小儿神经内科,辽宁沈阳110004;4.辽宁中医药大学学生工作处,辽宁沈阳110847

收稿日期:2012-06-04 发布日期:2012-11-28
通讯作者: 吴景东(Tel:024-31207258,E-mail：lnzywjd0719@163.com) E-mail:lnzywjd0719@163.com
作者简介:陶冶（1984-）|女|辽宁省丹东市人|在读医学博士|主要从事中医美容学方面的研究。
基金资助:
辽宁省科技厅自然科学基金资助课题（20102149）

Inhibitory effect of gypenosides on expressions of p38MAPK protein and c-Jun mRNA in photoaging HaCaT cells

TAO Ye¹,ZHANG Xiao-qing²,WU Qiong³,WU Jing-dong⁴

1.Department of Basic Theory of Traditional Chinese Medicine,School of Basic Medical Sciences,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;2. Department of Acupuncture| School of Acupuncture and Massage,Liaoning University of Traditional Chinese Medicine,Shenyang 110847, China;3.Department of Pediatrics Neurology,Affiliated ShengjingHospital,China MedicalUniversity,Shengang 110004,China;4.Student Affair Office,Liaoning University of Traditional Chinese Medicine,Shenyang 110847, China）

Received:2012-06-04 Published:2012-11-28

摘要/Abstract

摘要：

目的：观察绞股蓝总皂苷含药血清对光老化HaCaT细胞p38促分裂原活化蛋白激酶(p38MAPK)蛋白和Jun基因（c-Jun ）mRNA表达的影响，探讨绞股蓝总皂苷含药血清抗皮肤光老化的作用机制。方法：采用中波紫外线(UVB)照射建立光老化HaCaT细胞模型，加低、中、高剂量绞股蓝总皂苷含药血清于光老化HaCaT细胞模型培养液中培养，并设空白对照组、空白血清组和UVB模型组。采用Western blotting和RT-PCR方法检测各组细胞p38MAPK蛋白和c-Jun mRNA的表达水平。结果：与空白对照组比较，UVB模型组细胞p38MAPK蛋白和c-Jun mRNA表达显著上调，差异有统计学意义（P<0.01）；与UVB模型组比较，低、中和高剂量含药血清组细胞p38MAPK蛋白和c-Jun mRNA表达显著下调,差异有统计学意义（P<0.01）；与空白血清组比较，低、中和高剂量含药血清组细胞p38MAPK蛋白和c-Jun mRNA表达下降(P<0.01)。结论：绞股蓝总皂苷含药血清可能通过抑制p38MAPK蛋白和c-Jun mRNA表达抑制皮肤光老化。

关键词: 光老化；绞股蓝总皂苷；角质形成细胞；中波紫外线；p38促分裂原活化蛋白激酶；Jun基因

Abstract:

Objective To observe the effect of gypenosides-containing serum on the expressions of p38 mitogen-activated protein kinase（ p38MAPK）protein and c-Jun mRNA in photoaging HaCaT cells and to explore the mechanism of inhibitory effect of gypenosides-containing serum on photoaging.Methods The photoaging HaCaT cell model was established by UVB radiation, low,middle,and high doses of gypenosides-containing serum were used in the culture fluid of phatoaging HaCaT cell models;meanwhile blank control group,blank serum group and UVB model group were set up.The expression levels of p38MAPK protein and c-Jun mRNA of cells in each group were measured by Western blotting and RT-PCR methods. Results Compared with blank control group,the expression levels of p38MAPK protein and c-Jun mRNA of the cells inUVB model group were significantly increased,the difference had statistical significance（P<0.01）.Compared with UVB model group,the expression levels of p38MAPK protein and c-Jun mRNA of cells in low,middle and high doses of gypenosides-containing serum groups were significantly decreased,the difference had statistical significance（P<0.01）.Compared with blank serum group,the expression levels of p38 MAPK protein and c-Jun mRNA of the cells in low,middle and high doses of gypensides-containing serum groups were decreased(P<0.01).Conclusion Gypenosides-containing serum could prevent the skin photoaging by inhibinting the expressions of p38MAPK protein and c-Jun mRNA.

Key words: photoaing, gypenosides;HaCaT, ultraviolet B;p38MAPK, c-Jun

中图分类号:

R758.14

陶冶,张小卿,吴琼,吴景东. 绞股蓝总皂苷对光老化HaCaT细胞中p38MAPK蛋白和c-Jun mRNA表达的抑制作用[J]. J4, 2012, 38(6): 1147-1151.

TAO Ye,ZHANG Xiao-qing,WU Qiong,WU Jing-dong. Inhibitory effect of gypenosides on expressions of p38MAPK protein and c-Jun mRNA in photoaging HaCaT cells[J]. J4, 2012, 38(6): 1147-1151.

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