关键词: 富血小板血浆提取液, 脂肪干细胞, 细胞增殖, 大鼠, Wistar

Abstract: Abstract:Objective To explore the effect of allogenic platelet-rich plasma(PRP) extraction solution on the proliferation of adipose-derived stem cells(ADSCs) in vitro and to provide an experimental evidence for wound healing by allogenic PRP extracting solution.Methods The adipose tissue derived from SD rats was isolated,cultured and passaged.The cells harvested above were cultured respectively in adipose-,osteo- and neuro- lineages inducing culture solution.The rats’ blood was extracted and prepared by three times’ centrifugation for making PRP and different concentrations of allogenic PRP extraction solution (6.67%,3.35%,and 1.67% volume fractions) were prepared by DMEM/F12. The third passage of ADSCs were divided into 4 groups.The ADSCs in experimental groups (A,B and C groups) were respectively cultured with 6.67%,3.35% and 1.67% volume fractions of PRP extraction solution.The cells in control group (D group) were cultured with 10% fetal bovine serum and DMEM/F12.The proliferative activities of the ADSCs were detected by MTT after 24 and 48 h culture. Results The harvested cells appeared to be adherent and fibroblast-like,which were successfully obtained and culured from rat adipose tissue.The cells could differentiate into adipocytes,osteocytes and neurocytes by adipose-inducing,osteo-inducing,and neuro-inducing.It was proved that the cells had the potential of differentiating into adipose-,osteo- and neuro- lineage cells and the harvested cells were ADSCs.The A values assayed by MTT after 24 h in  A,B,C and D groups were 0.434 3±0.084 3,0.351 6±0.076 6,0.258 1±0.060 0, and 0.259 2±0.073 6,respectively;and the values after 48 h were 1.018 6±0.443 6,0.741 7±0.109 7,0.367 8±0.034 2,and 0.395 7±0.106 2,respectively.The A values in A group and B group were significantly higher than those in C group and D group (P<0.05),but there was no statistically significant difference between C and D groups(P>0.05).Compared with D group,the proliferation rates in A group and B group were significantly increased,but it showed negative increase in C group.Conclusion Allogenetic PRP extraction solution with suitable concentration can enhance the proliferation of  ADSCs  in vitro
,which is expected to accelerate wound repaire.