吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (2): 405-409.doi: 10.7694/jldxyxb20130249

• 基础研究 • 上一篇    下一篇

长春地区部分医院革兰阴性杆菌氨基糖苷类修饰酶基因的检测

周佳琦,卢佳睿*,徐 花,韩 放,孙延波   

  1. 吉林大学白求恩医学院病原生物学系,吉林 长春 130021
  • 收稿日期:2012-11-02 出版日期:2013-03-28 发布日期:2013-03-26
  • 通讯作者: 孙延波(Tel: 0431-85619574, E-mail: sunyb@jlu.edu.cn) E-mail:sunyb@jlu.edu.cn
  • 作者简介:周佳琦(1989-),女,吉林省东丰县人,在读理学硕士,主要从事细菌耐药分子机制的研究。
  • 基金资助:

     国家自然科学基金委主任基金资助课题(81150037)

Detection of aminoglycoside modifying enzyme genes in gram-negative bacilli from some hospitals in Changchun area

ZHOU Jia-qi,LU Jia-rui*,XU Hua,HAN Fang,SUN Yan-bo   

  1. Department of Pathogenobiology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China
  • Received:2012-11-02 Online:2013-03-28 Published:2013-03-26

摘要: 目的:分析长春地区部分医院革兰阴性杆菌氨基糖苷类修饰酶基因的分布和类型,为抗生
素的合理应用提供依据。方法:通过琼脂稀释法筛选出阿米卡星与庆大霉素的耐药菌株,采用聚合
酶链式反应及序列分析的方法检测耐药菌株所携带的氨基糖苷类修饰酶基因类型。结果:69株革兰阴性杆菌对阿米卡星与庆大霉素的耐药率分别为44.9%和87.0%,其中63株 (91.3%) 检出氨基糖苷类修饰酶基因,基因类型包括aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰb、ant(3")-Ⅰ和
ant(2")-Ⅰ,阳性率分别为4.3%、81.2%、43.5%、33.3%和13.0%;而aac (6′)-Ⅱ基因为阴性。结论:本地区革兰阴性杆菌氨基糖苷类修饰酶基因主要以aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰb、ant(3")-Ⅰ和ant(2")-Ⅰ  5种类型存在,其中以aac(3)-Ⅱ型氨基糖苷类修饰酶基因最为常见。合理应用氨基糖苷类抗生素对于降低细菌的耐药性具有重要意义。

关键词: 革兰阴性杆菌, 氨基糖苷类修饰酶, 耐药性

Abstract: Objective To analyze the distribution and genotyping of aminoglycoside modifying enzyme genes in gram-negative bacilli from some hospitals in Changchun area and to provide a reasonable basis for the application of antibiotics. Methods Amikacin-resistant and gentamicin-resistant strains were screened using agar dilution tests,and the aminoglycoside modifying enzyme genes were amplified by PCR and analyzed by sequencing.
Results The resistant rates of 69 strains of gram-negative bacilli to  amikacin and gentamicin were 44.9% and 87.0%,respectively.63 strains of gram-negative bacilli (91.3%) were aminoglycoside modifying enzyme-positive,the genotypes of aminoglycoside modifying enzymes included aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb,ant(3")-Ⅰ,and ant(2")-Ⅰ,with positive rates of 4.3%,81.2%,43.5%,33.3%,and 13.0%,respectively.But the aac(6′)-Ⅱ was not found.
Conclusion The genotypes of aminoglycoside modifying enzymes of gram-negative bacilli in local area are aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb,ant(3")-Ⅰ,and ant(2")-Ⅰ,and the most common of aminoglycoside modifying enzyme genes is aac(3)-Ⅱ.Reasonable application of aminoglycosid
e antibiotics to reduce bacterial resistance has great significance.

Key words: gram-negative bacilli, aminoglycoside modifying enzymes, antibiotic-resistance

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