吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (2): 414-418.doi: 10.7694/jldxyxb20130251

• 基础研究 • 上一篇    下一篇


紫外分光光度法在没食子酸体外还原Fe3+能力检测中的应用及其意义

伍翔群1,于 兰2,于 杰3,黄彩婷1,许维国1,牛凤兰1   

  1. 1.吉林大学公共卫生学院卫生检验学教研室,吉林 长春 130021;2.长春中医药大学附属医院脑病科,吉林 长春 130021;3.吉林大学中日联谊医院电诊科,吉林 长春 130033
  • 收稿日期:2012-05-21 出版日期:2013-03-28 发布日期:2013-03-26
  • 通讯作者: 甄 清(Tel:0431-85619451,E-mail:zq415@sina.com) E-mail:zq415@sina.com
  • 作者简介:伍翔群(1989-),女,内蒙古自治区包头市人,在读医学硕士,主要从事天然植物活性与毒性研究。
  • 基金资助:

    国家重点基础研究发展计划(973计划)资助课题(2011CB935800);吉林省科技厅重点项目资助课题(20100905);吉林省高技术产业发展项目资助课题(2010014)

Application of UV spectrophotometer  in detection of  ability of gallic 
  acid to restore Fe3+ in vitro 

WU Xiang-qun1,YU Lan2,YU Jie3,HUANG Cai-ting1,XU Wei-guo1,NIU Feng-lan1   

  1. 1.Department of Inspection,School of Public Health,Jilin University,Changchun 130021,China;2.Encephalopathy Division,First Affiliated Hospital,Changchun Univeristy of Chinese Medicine,Changchun 130021,China;3.Department of Electric Diagnosis,China-Japan Union Hospital,Jilin
    University,Changchun 130033,China
  • Received:2012-05-21 Online:2013-03-28 Published:2013-03-26

摘要: 目的:测定没食子酸(GA)体外还原Fe3+的能力,并与公认的抗氧化剂抗坏血酸(Vc)比较,探讨其体外抗氧化性,为GA抗氧化应用提供实验依据。方法:采用不同浓度的GA(0.04、0.08、0.12和0.16 g/L-1)和Vc(0.04、0.08、0.12和0.16 g/L-1)在邻二氮菲-Fe2+体系发生Fenton反应,在铁氰化钾-Fe3+体系发生
Fe3+还原反应,用紫外分光光度计分别检测反应后溶液的吸光度(A)值,应用SPSS 17.0软件包采用双因素方差分析溶液A值和GA及
Vc浓度的关系。结果:在邻二氮菲-Fe2+体系中,同浓度的GA 与Vc比较,GA对Fe3+的还原能力强于Vc(P<0.05);与0.04 g/L-1GA比较,0.08、0.12和0.16 g?L-1
GA对Fe3+还原能力逐渐增强(P<0.05);且乙醇对该体系的A值有影响。在铁氰化钾-Fe3+体系中,当GA 与Vc浓度均为0.04 g/L-1
时,GA对Fe3+还原能力强;而其余浓度下其还原能力均弱于Vc(P<0.05)。当盐酸浓度大于0.84×10-2mol?L-1时,其对铁氰化钾-Fe3+体系的A值有影响。结论:与Vc比较,GA在体外环境对Fe3+有较强还原能力。

关键词: 没食子酸, 抗坏血酸, 邻二氮菲-Fe2+体系, 铁氰化钾-Fe3+体系, 分光光度比色法

Abstract: Objective  To detect the ability of gallic acid (GA) to  restore Fe3+ in vitro,and  explore its antioxidant activity in vitro an
d to compare it with recognized antioxidant ascorbic acid (Vc),and to provide experimental basis for antioxident application of GA.
Methods Different concentrations of GA (0.04,0.08,0.12 and 0.16 g/L-1) and Vc (0.04,0.08,0.12 and 0.16 g/L-1) were used in the phenant
hroline-Fe2+ system to  occurre Fenton reaction and the potassium ferricyanide-Fe3+ system to occurre Fe3+ restore reaction.The A values of the solution after reaction were detected with  UV spectrophotometer. SPSS 17.0 package and two-way ANOVA were used to analyze the relationship between the A values and concentrations of GA and Vc solution.Results  In the phenanthroline-Fe2+ system,the A value of  GA solution was larger than that of Vc at the same concentration(P<0.05).Compared with 0.04 g/L-1 GA group,the A values in 0.08,0.12,and 0.16 GA groups were gradually increased(P<0.05);and ethanol influenced the A value of the phenanthroline-Fe2+ system.In potassium ferricyanide-Fe3+ system,the Fe3+ reduction ability of GA was stronger than Vc when their concentrations were  0.04 g?L-1;the abilities were weaker  in the other concentrations.When the concentration of HCl was higher than 0.84× 10-2mol/L-1,it had  great influence in the A value of potassium ferricyanide-Fe3+ system.Conclusion Compared with Vc,GA has a stronger Fe3+ restoring capacity under certain conditions in vitro.

Key words: gallic acid, ascorbic acid, phenanthroline-Fe2+ system, potassium ferricyanide-Fe3+ system, spectrophotometric assay

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