关键词: 合募配穴, 针刺, 应激性胃溃疡 , 差异表达蛋白

Abstract: To observe the influence of different acupoints of preventive acupuncture on gastric tissue damage of stress ulcer rat and  protein mass spectrometry,and to clarify the effect of different acupoints of preventive acupuncture on preventing stress ulcer,and  to obtain the related diffierential  expression proteins.Methods 120 male Wistar rats were randomly divided into normal control group (n=15),bundled control group (n=15),stress ulcer model group(n=15),Mu point group(n=15),Xiahe point group (n=15),He and Mu points group(n=15),Back-shu group(n=15),and Back-shu and Mu points group(n=15).The stress ulcer model was constructed by water-immersion method after acupuncture.The morphological changes of the damage of the rat gastric mucosa were observed,and the ulcer index was assessed in various groups.The stomach tissue proteins were extracted,and the proteins in supernatant of stomach tissue homogenate were separated  by SDS-PAGE gel electrophoresis method. Nanoliter electrospray mass high-performance liquid  instrument combined with  LTQ linear ion trap mass spectrometer (Eksigent-Thermo LTQ-MS)  was used to detect the expression levels of proteins. Results The ulcer index of the rats in  He and Mu points group was lower than those  in Mu point group, Xiahe point group,Back-shu point  group, Back-shu and Mu points group(P<0.05). After screening,the interesting differential expression proteins were obtained as follows:①Enzymes,including glyceraldehyde 3-phosphate dehydrogenase (GAPDH),carbonic anhydrase 1 (Car1),carbonic anhydrase 3 (Car3),aconitum synthase (Aco2movement),alpha-enolase (of Eno1),beta enolase (Eno3),and gamma enolase;②Skeleton proteins,including alpha1 actin protein (Actc1),beta actin (Actb),α-actin protein (Acta1),and 43 000 protein (Actg2);
③ Transport proteins,including serum transferrin (TF subtype 1) and calreticulin (Calr);④Heat shock protein,including 10 000 heat shock protein (Hspe1).Conclusion The preventive  effect of  He and  Mu points on stress ulcer  is better than those of single point  and Back-shu and Mu points,its mechanism may be associated with a variety of protein expression changes.

Key words: Xiahe and Mu points, acupuncture, stress ulcer, differentially expressed proteins