吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

顺铂对胶质瘤神经球的凋亡诱导作用及其机制

齐玲1,温娜2,杨淑艳1,赵东海1,王玮瑶1,韩磊1,金宏2   

  1. (1.吉林医药学院病理学教研室,吉林 吉林 132013;2.吉林医药学院实验中心,吉林 吉林  132013)
  • 收稿日期:2013-04-05 发布日期:2013-11-28
  • 通讯作者: 金 宏 E-mail:(Tel:0432-64560384,E-mail:jinhong0706@sina.com)
  • 作者简介:齐 玲(1974-),女,吉林省吉林市人,副教授,医学博士,主要从事肿瘤药物研究。
  • 基金资助:

    国家自然科学基金资助课题(81201671);吉林省教育厅科研基金资助课题(2012330,2011281)

Induction of cisplatin on apoptosis of glioma neurospheres  and its mechanism


QI Ling1,WEN Na2,YANG Shu-yan1,ZHAO Dong-hai1,WANG Wei-yao1, HAN Lei1,JIN Hong2   

  1. (1.Department of Pathology,Jilin Medical College,Jilin 132013,China;2. Experimental Center,Jilin Medical College,Jilin 132013,China)
  • Received:2013-04-05 Published:2013-11-28

摘要:

目的:研究顺铂对人脑胶质瘤SHG-44神经球的诱导凋亡作用,探讨其诱导凋亡的机制。方法:培养人脑胶质瘤SHG-44神经球,将其分为对照组和顺铂组,对照组细胞给予干细胞培养液,顺铂组细胞在对照组基础上给予10 mg•L-1顺铂作用24、48和72 h后,MTT法检测SHG-44神经球生长抑制率;倒置显微镜观察2组细胞凋亡情况;ELISA法检测2组SHG-44神经球分泌Bax、Bcl-2和caspase-3蛋白水平。结果:与对照组比较,10 mg•L-1 顺铂作用SHG-44神经球24、48和72 h时的细胞生长抑制率均明显升高(P<0.01)。倒置显微镜下,顺铂组神经球数量明显减少,并可见到明显的凋亡小体。与对照组比较,10 mg•L-1 顺铂作用SHG-44神经球72 h,培养上清中Bax和Bcl-2分泌量均降低,caspase-3分泌量增加,Bax/Bcl-2比值明显升高(P<0.05或P<0.01)。结论:顺铂可以明显地抑制SHG-44神经球生长,其机制可能是通过上调Bax/Bcl-2比值,使促凋亡因素占优势,从而使神经球发生凋亡。

关键词: 顺铂, 胶质瘤, 神经球, 凋亡, Bax/Bcl-2

Abstract:

Abstract:Objective
To study the induction of cisplatin on apoptosis of human glioma SHG-44 neurospheres,and to explore its mechanism.Methods The SHG-44 neurospheres were cultured and divided into control and cisplatin groups.The cells in  control group were treated with stem cells media,and based on control group,the cells in cisplatin group were treated with 10 mg•L-1 cisplatin for 24,48 and 72 h.MTT assay was used to evaluate the  inhibitory rate of proliferation of SHG-44 neurospheres;the morphology of the  cells  was observed by inverted microscope;the levels of Bax,Bcl-2,and caspase-3 proteins secreted by SHG-44 neurospheres  were detected by ELISA assay.Results Compared with control group,the  inhibitory rates of SHG-44 neurospheres in cisplatin group were increased  after treated for 24,48 and 72 h(P<0.01).The apoptotic bodies were observed and the number of neuroshperes were reduced in cisplatin group  under inverted microscope.Compared with control group,the levels of Bax and Bcl-2 were decreased,the level of caspase-3  was increased,and the ratio of Bax/Bcl-2  was significantly increased in cisplatin group (P<0.05 or P<0.01).Conclusion Cisplatin could significantly inhibit the growth of SHG-44 neurospheres and induce apoptosis,and its mechanism may be related to  up-regulating the ratio of Bax/Bcl-2 and giving the apoptosis factors the advantage.

Key words: cisplatin, glioma, neurospheres, apoptosis, Bax/Bcl-2

中图分类号: 

  • R739.4