吉林大学学报(医学版)

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厄贝沙坦对病毒性心肌炎慢性期小鼠心肌组织中periostin表达的影响及其抗心肌纤维化作用机制

吴 岚,张春艳,孙景辉,宋丽君   

  1. 吉林大学第一医院儿科,吉林 长春 130021
  • 收稿日期:2013-03-19 发布日期:2013-12-12
  • 通讯作者: 宋丽君(Tel:0431-88783239,E-mail:songlijun191@sina.com) E-mail:songlijun191@sina.com
  • 作者简介:吴 岚(1979-),女,吉林省长春市人,主治医师,医学博士,主要从事病毒性心肌炎方面的研究。 
  • 基金资助:

    吉林省科技厅重点项目资助课题(20120964)

Effect of irbesartan on periostin expression in myocardium tissue of  VMC mice in chronic phase and its mechanism of antimyocardial fibrosis

WU Lan,ZHANG Chun-yan,SUN Jing-hui,SONG Li-jun   

  1. Department of Pediatrics,First Hospital,Jilin University,Changchun 130021,China
  • Received:2013-03-19 Published:2013-12-12

摘要:

目的:探讨厄贝沙坦对病毒性心肌炎(VMC)慢性期小鼠心肌组织中periostin表达及心肌纤维化的影响,阐明其抗心肌纤维化的可能机制。方法:以柯萨奇病毒B3(CVB3)感染40只BALA/c小鼠建立VMC慢性期动物模型,21只模型小鼠随机分为模型组(n=11)和厄贝沙坦组(n=10),另选10只BALA/C小鼠作为对照组。模型组和对照组小鼠给予自来水(1 mL/d),厄贝沙坦组小鼠给予厄贝沙坦50 mg/kg/d,60 d后检测3组小鼠左室舒张末期内径(LVEDd)和左室收缩末期内径(LVEDs),并计算左室射血分数缩短率(FS);ELISA法检测各组小鼠血清血管紧张素Ⅱ(Ang Ⅱ)浓度,对3组小鼠的心脏切片行MASSON染色,计算心肌胶原容积分数(CVF);应用RT-PCR法对TGF-β1和periostin在小鼠心肌组织中的表达水平进行半定量分析。结果:与对照组比较,模型组小鼠LVEDd和LVEDs显著升高(P<0.01),FS显著下降(P<0.01),血清AngⅡ浓度、CVF、TGF-β1 mRNA和periostin mRNA表达均显著升高(P<0.01);与模型组比较,厄贝沙坦组LVEDd和LVEDs降低(P<0.05),FS升高(P<0.05),血清Ang Ⅱ浓度无明显差异(P>0.05),CVF、TGF-β1 mRNA和periostin mRNA表达水平下降(P<0.05)。结论:厄贝沙坦具有抗VMC慢性期小鼠心肌纤维化的作用,其作用机制可能是通过抑制心肌组织中TGF-β1表达,进而减少periostin表达水平而实现的。

关键词: 病毒性心肌炎, 心肌纤维化, 厄贝沙坦, periostin

Abstract:

Abstract:Objective To explore the  effects of irbesartan on exrpession of periostin in  myocardium  tissue of  the viral myocarditis (VMC) mice  in chronic phase and myocardial fibrosis,and to clarify the possible mechanism of its antimyocardial fibrosis.Methods The models of VMC in chronic phase were founded by injecting coxsackie virus B3(CVB3) solution into 40 BALA/c mice. 21 VMC mice were divided into model group(n=11)and irbesartan group(n=10).And at the same time, 10 BALA/C mice were used as control group.The mice in model group and control group were fed with water (1 mL?d-1),and the mice in irbesartan group were fed with irbesartan at a dose of 50 mg?kg-1?d-1.After 60 d,the levels of left ventricular end-diastolic dimension (LVEDd),left ventricular end-systolic dimension (LVEDs) and left ventricular fractional shortening (FS) of the mice in 3 groups were examined;the concentrations  of AngⅡ in the serum of the mice in various groups were detected by ELISA method;the myocardium tissue of mice were stained by MASSON and cardiac collagen volume fraction(CVF) was accounted.At the same time,the contents of periostin mRNA and TGF-β1 mRNA in myocardium tissue of mice were detected by RT-PCR method.Results Compared with control group,the levels of  LVEDd and LVEDs were  increased (P<0.01),and the level of FS was decreased (P<0.01) in model group;the levels of serum Ang Ⅱ,CVF,TGF-β1 mRNA,and periostin mRNA were increased (P<0.01)in model group.Compared with model group,the levels of LVEDd  and  LVEDs of the mice in irbesartan group were obviously decreased(P<0.05),and the level of FS was increased(P<0.05).There was no significant difference of the levels of serum Ang Ⅱ between irbesartan group and model group(P>0.05),but the levels of CVF,TGF-β1 mRNA,and periostin mRNA of the mice in irbesartan group were obviously decreased compared with model group(P<0.05).Conclusion Irbesartan can offer some protection to myocardial fibrosis inVMC mice. The  probable mechanisms may be acted through  inhibiting  the expression of TGF-β1 in myocardium tissue to reduce the expression of  periostin.

Key words: viral myocarditis, myocardial fibrosis, irbesartan, periostin

中图分类号: 

  • R972.9