关键词: 白附子提取物, 胶质瘤细胞, 细胞增殖

Abstract:

Abstract:Objective To study the influence of Rhizoma typhonii extract on the growth of glioma SHG-44 cells cultured in vitro,and to explore the mechanism of the  inhibitory effect of Rhizoma typhonii extract on the growth of glioma cells.Methods The SHG-44 cells were cultured and divided into blank control group and 8,40,200,1 000  μg/L Rhizoma typhonii extract groups.The inhibitory effect of Rhizoma typhonii extract on the growth of glioma SHG-44 cells was measured by MTT assay.The secretion levels of Bax and caspase-3 proteins were examined by ELISA assay.The expression level of caspase-3 protein was examined by Western blotting method.Results Compared with blank control group,the inhibitory rates of the growth of SHG-44 cells in 200,1 000 μg?L-1 Rhizoma typhonii extract groups at 24　h,and 8,40,200, and 1 000 μg/L Rhizoma typhonii extract groups at 48 h were significantly increased (P<0.05 or P<0.01).The secretion levels of Bax and caspase-3 proteins in 40,200, and 1 000 μg/L Rhizoma typhonii extract groups at 48 h were increased compared with blank control group (P<0.05 or P<0.01).Compared with blank control group,the expression levels of caspase-3 protein in different doses of  Rhizoma typhonii  extract groups were increased significantly(P<0.01).Conclusion Rhizoma Typhonii extract can inhibit the growth of cells through up-regulating the expression of Bax protein,increasing the expression level of caspase-3 protein and activating apoptosis pathway.

Key words: Rhizoma typhonii extract, glioma cells, cell proliferation