关键词:  , 肺血栓栓塞症；G20210A基因；凝血酶原

Abstract:

Objective To study the incidence frequency of prothrombin G20210A (FⅡ G20210A) mutation in the patients with pulmonary thromboembolism(PTE) in northeast China,and to clarify thepredictive value of FⅡ G20210A mutation detection in PTE of the population in northeast China.
Methods 60  PTE patients(PTE group) and 80 sex-matched healthy controls(control group) from the same geographic area were selected.All
the patients were diagnosed by lung ventilation/perfusion scan and/or multi-slice CT pulmonary angiography(CTPA) as well as medical history.The genome DNA was extracted from the whole blood using alcohol.Polymerase chain reaction(PCR),restriction fragment length polymorphisms(RFLP)  analysis with HindⅢ restriction enzyme and sepharose gel electrophoresis were used to identify the FⅡ G20210A mutation in PTE group and control group.
Results After digested by HindⅢ restriction enzyme,only the fragments of  407 and 99 bp were found in PTE group.The frequency of
 FⅡ G20210A mutation was 0%,there was no statistical difference compared with contol group(P＞0.05).There were no heterozygote and homozygote mutation of FⅡ G202210A gene in PTE group and control group.Conclusion The incidence of FⅡ G20210A mutation in the PTE patients in northeast China is very low,and the detection  of  FⅡ  G20210A mutation may have no predictive  value in PTE of the population in northeast China.

Key words: pulmonary thromboembolism, G20210A gene, prothrombin