邻苯二甲酸二丁酯对雄性小鼠生殖系统的脂质过氧化作用

doi:10.13481/j.1671-587x.20150323

摘要/Abstract

摘要：

目的:观察邻苯二甲酸二丁酯(DBP)对雄性小鼠生殖系统的脂质过氧化作用和对抗氧化酶活性的影响,探讨其对雄性小鼠生殖系统的可能作用机制。方法:40只健康雄性ICR小鼠随机分为对照组和200、400和800 mg·kg^-1DBP组,每组10只。各剂量DBP组小鼠连续灌胃给予DBP,灌胃剂量分别为200、400和800 mg·kg^-1·d^-1,对照组小鼠仅给予玉米油溶剂。于染毒满2和4周时各组分别处死5只小鼠,收集睾丸,并测定睾丸组织中丙二醛(MDA)水平及谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性。结果:染毒2周后,400和800 mg·kg^-1 DBP组小鼠MDA水平明显高于对照组和200 mg·kg^-1 DBP组(P<0.05);400和800 mg·kg^-1 DBP组小鼠睾丸组织中SOD活性低于200 mg·kg^-1 DBP组(P<0.05);各剂量DBP组小鼠睾丸组织中GSH-Px活性均明显低于对照组(P<0.05)。染毒4周后,400和800 mg·kg^-1 DBP组小鼠睾丸脏器系数低于对照组和200 mg·kg^-1DBP组(P<0.05);400和800 mg·kg^-1 DBP组小鼠睾丸组织中MDA水平均高于对照组(P<0.05),200 mg·kg^-1 DBP组小鼠睾丸组织中MDA水平低于对照组(P<0.05);800 mg·kg^-1 DBP组小鼠睾丸组织中SOD活性明显低于对照组和200、400 mg·kg^-1 DBP组(P<0.05);各剂量DBP组小鼠睾丸组织中GSH-Px活性均明显高于对照组(P<0.05)。结论:DBP可诱导小鼠睾丸产生脂质过氧化反应,诱导氧化应激,导致睾丸抗氧化能力下降,造成生殖功能障碍。

关键词: 邻苯二甲酸二丁酯, 丙二醛, 谷胱甘肽过氧化物酶, 超氧化物歧化酶, 脂质过氧化作用

Abstract:

Objective To observe the lipid peroxidation of dibutylphthalate(DBP) on the reproductive system of the male mice and its influence in the antioxidant enzyme activities, and to investigate the possible mechanism of DBP to the reproductive system of the male mice. Methods 40 healthy male ICR mice were randomly divided into control group, 200 mg·kg^-1 DBP group, 400 mg·kg^-1 DBP group, and 800 mg·kg^-1 DBP group(n=10).The mice in DBP groups were administrated with 200, 400 and 800 mg·kg^-1·d^-1 DBP by gavage.The mice in control group were only received corn solvent.5 mice in each group were sacrificed respectively 2 and 4 weeks later, and their testis were collected.The malondialdehyde (MDA) levels, activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in testis tissue of the mice in various groups were detected. Results Two weeks after exposure, the MDA levels in testis tissue of the mice in 400 and 800 mg·kg^-1 DBP groups were significantly higher than those in control group and 200 mg·kg^-1 DBP group(P<0.05);the SOD activities in 400 and 800 mg·kg^-1 DBP groups were lower than that in 200 mg·kg^-1 DBP group (P<0.05).Four weeks after exposure, the testis organ coefficients in 400 and 800 mg·kg^-1 DBP groups were lower than those in control group and 200 mg·kg^-1DBP group (P<0.05);the MDA levels in 400 and 800 mg·kg^-1 DBP groups were higher than that in control group (P<0.05), and the MDA level in 200 mg·kg^-1 DBP group was lower than that in control group (P<0.05).The SOD activity in testis tissue of the mice in 800 mg·kg^-1DBP group was significantly lower than those in control group, 200 mg·kg^-1 DBP group and 400 mg·kg^-1 DBP group (P<0.05);the GSH-Px activities in testis tissue of the mice in DBP groups were significantly higher than that in control group (P<0.05). Conclusion DBP can induce the lipid peroxidation of the mouse testis and induce oxidative stress, and lead to the descending of testicular antioxidant capacity, then Results in reproductive dysfunction.

Key words: dibutylphthalate, malondialdehyde, glutathione peroxidase, superoxide dismutase, lipid pexoxidation

中图分类号:

R114

吴冠宇, 王硕, 刘艳, 赵淑华. 邻苯二甲酸二丁酯对雄性小鼠生殖系统的脂质过氧化作用[J]. 吉林大学学报(医学版), 2015, 41(03): 553-557.

WU Guanyu, WANG Shuo, LIU Yan, ZHAO Shuhua. Lipid peroxidation of dibutylphthalate on reproductive system of male mice[J]. Journal of Jilin University Medicine Edition, 2015, 41(03): 553-557.

参考文献

[1] Bergh C, Torgrip R, Emenius G, et al. Organophosphate and phthalate esters in airand settled dust-a multi-location indoor study[J].Indoor Air, 2011, 21(1):67-76.

[2] Meeker JD.Exposure to environmental endocrine disrupting compounds and men's health [J].Maturitas, 2010, 66(3):236-241.

[3] KambiaN, DineT, GressierB, et al.Strong variability of di(2-ethylhexyl)phthalate (DEHP) plasmatic rate in infants and children undergoing 12-hour cyclic parenteral nutrition [J].J Parenteral Enteral Nutrit, 2013, 37(2):229-235.

[4] 武杰, 吴希庆, 萨音白刚.邻苯二甲酸酯类塑化剂毒性研究进展[J].包头医学院学报, 2013, 29(5):121-123.

[5] 李俊儒, 胡继红, 段琼, 等.我国邻苯二甲酸酯类检测方法研究进展[J].食品与发酵工业, 2014, 40(2):180-183.

[6] 秦晓东, 袁欣然, 李冬梅, 等.邻苯二甲酸酯类的雄性生殖毒性研究进展[J].医学研究生学报, 2014, 27(5):531-535.

[7] 董蕾, 张明.土壤中邻苯二甲酸二丁酯研究进展[J].安徽农学通报(上半月刊), 2011, 17(3):122-123.

[8] 李蕾, 宋乃宁, 李海山, 等.邻苯二甲酸酯类毒理学效应及其机制研究进展[J].中国药理学与毒理学杂志, 2014, 28(3):468-474.

[9] Hines CJ, HopfNB, DeddensJA, et al.Occupational exposure to diisononyl phthalate (DiNP) in polyvinyl chloride processing operations [J].Int Arch Occup Envir Health, 2012, 85(3):317-325.

[10] 王凯荣, 崔明明, 史衍玺.农业土壤中邻苯二甲酸酯污染研究进展[J].应用生态学报, 2013, 24(9):2699-2708.

[11] Yao PL, LinYC, Richburg JH.Transcriptional suppression of Sertoli cell Timp2 in rodents following mono-(2-ethylhexyl) phthalate exposure is regulated by CEBPA and MYC [J].Biol Reprod, 2011, 85(6):1203-1215.

[12] Pant N, PantA, ShuklaM, et al.Environmental and experimental exposure of phthalate esters: the toxicological consequence on human sperm[J].Hum Exp Toxicol, 2011, 30(6):507-514.

[13] ErdosZ, PearsonK, GoedkenM, et al.Inhibin B response to testicular toxicants hexachlorophene, ethane dimethanesulfonate, di-(n-butyl)-phthalate, nitrofurazone, DL-ethionine, 17-alpha ethinylestradiol, 2, 5-hexanedione, or carbendazim following short-term dosing in male rats[J]. Birth Defects Res B Develop Reprod Toxicol, 2013, 98(1):41-53.

[14] 吴泽颖, 朱烨, 李威, 等.塑料制品中邻苯二甲酸酯类增塑剂的快速筛选[J].塑料科技, 2014, 42(9):97-101.

[15] DobrzyńskaMM, Tyrkiel E, Hernik A, et al.The effects of dibutylphthalate on the somatic cells of laboratory mice[J].Panstwowy Zaklad Higieny, 2010, 6(11):13-19.

[16] 郭书周, 杨自军, 张才.高钼低铜对小鼠睾丸组织脂质过氧化作用的影响[J].河南农业科学, 2011, 40(11):145-147.

[17] 王婷, 郑玉建, 吴顺华, 等.砷致小鼠脂质过氧化及锌、硒对其的干预研究[J].新疆医科大学学报, 2007, 30(6):551-553.

[18] 董亮, 何永志, 王远亮, 等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技导报, 2013, 15(5):53-58.

[19] 王玉邦, 宋玲, 朱正平, 等.邻苯二甲酸二丁酯对大鼠睾丸生化酶及脂质过氧化的影响[J].中华男科学杂志, 2004, 10(10):729-733.

[20] 白苇, 韩国宏.肝细胞癌的综合介入治疗[J].临床肝胆病杂志, 2013, 29(1):36-37.

[21] 陈洁, 姚敏, 姚登福.磷脂酰肌醇蛋白多糖-3介导的信号道路与肝癌靶向治疗[J].临床肝胆病杂志, 2013, 29(1):77-80.