吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (06): 1130-1133.doi: 10.13481/j.1671-587x.20150606

• 基础研究 • 上一篇    下一篇

血管性痴呆大鼠海马齿状回区D1受体的表达

万朋1, 高俊涛1, 王丹2, 王师2, 金清华2   

  1. 1. 吉林医药学院生理学教研室, 吉林 吉林 132013;
    2. 延边大学医学院生理学与病理生理学教研室, 吉林 延吉 133002
  • 收稿日期:2015-03-17 发布日期:2016-01-11
  • 通讯作者: 金清华,教授,博士研究生导师(Tel:0433-2435131,E-mail:yqinghua@ybu.edu.cn) E-mail:yqinghua@ybu.edu.cn
  • 作者简介:万朋(1980-),男,吉林省农安县人,讲师,理学博士,主要从事神经生理学方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(31160211)

Expression of dopamine D1 receptors in hippocampal dentate gyrus in rats with vascular dementia

WAN Peng1, GAO Juntao1, WANG Dan2, WANG Shi2, JIN Qinghua2   

  1. 1. Department of Physiology, Jilin Medical University, Jilin 132013, China;
    2. Department of Physiology and Pathophysiology, College of Medical Sciences, Yanbian University, Yanji 133002, China
  • Received:2015-03-17 Published:2016-01-11

摘要:

目的: 观察血管性痴呆(VD)模型大鼠海马齿状回(DG)区的多巴胺(DA)浓度及D1受体表达情况,并探讨两者间的关系。方法:选用12只Sprague-Dawley雄性大鼠,随机分为VD组和假手术组,每组6只。利用双侧颈总动脉永久性结扎法制备VD大鼠模型,模型制备结束30 d后,利用脑部微量透析法和高效液相色谱法测定海马DG区细胞外液中的DA浓度,应用免疫组织化学法观察海马DG区D1受体的表达情况。结果:VD组大鼠海马DG区细胞外液中的DA浓度较假手术组明显降低(P<0.05)。在VD组和假手术组大鼠的海马DG区颗粒细胞层均有D1受体的表达,2组间比较差异无统计学意义(P>0.05);而2组大鼠海马门区均有D1受体的表达,VD组D1受体表达水平明显升高(P<0.05)。结论:VD模型大鼠海马DG区的DA浓度减少,其功能可能是通过海马门区D1受体的表达增加而代偿的。

关键词: 血管性痴呆, 海马齿状回, 受体,多巴胺D1, 大鼠,Sprague-Dawley

Abstract:

Objective To observe the extracellular content of dopamine (DA) and expression of D1 receptors in hippocampal dentage gyrus (DG) in the model rats with vascular dementia (VD),and to investigate the relationship between them. Methods 12 male SD rats were randomly divided VD group and sham-operation group,and the VD model was prepared by permanent bilateral carotid occlusion.The extracellular content of DA in the DG was determined by in vivo microdialysis and HPLC,and the expression of D1 receptors was measured by immunehisto-chemistry. Results The DA content in the DG of the rats in VD group was lower than that in sham-operation group (P<0.05).The number of D1 receptor-positive cells in the DG hilus in VD group was increased compared with sham-operation group (P<0.05),whereas the expression of D1 receptor in DG granule cell layer did not change (P>0.05). Conclusion The DA content in the hippocampal DG is decreased in the VD rats,and its function may be compensated by the up-regulation of D1 receptors in the DG hilus.

Key words: vascular dementia, dentate gyrus, receptors,dopamine D1, rats,Sprague-Dawley

中图分类号: 

  • R743