吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (01): 177-181.doi: 10.13481/j.1671-587x.20160136

• 调查研究 • 上一篇    下一篇

2011-2013年成都地区发热呼吸道症候群住院患儿鼻病毒流行病学特征

董泽丰1,2, 郑田利1, 范雪佳1, 裴晓方1   

  1. 1. 四川大学华西公共卫生学院华西第四医院, 四川成都 610041;
    2. 江苏省苏州市疾病预防控制中心, 江苏苏州 215004
  • 收稿日期:2015-04-19 发布日期:2016-01-26
  • 通讯作者: 裴晓方,教授,博士研究生导师(Tel:028-85503679,E-mail:xxpeiscu@163.com) E-mail:xxpeiscu@163.com
  • 作者简介:董泽丰(1989-),男,安徽省宿州市人,医学硕士,主要从事微生物基因结构及功能方面的研究。
  • 基金资助:

    国家卫生计生委科技重大专项项目资助课题(2012ZX10004-212)

Epidemic characteristics of rhinovirus in hospitalized children with respiratory syndrome in Chengdu during 2011-2013

DONG Zefeng1,2, ZHENG Tianli1, FAN Xuejia1, Pei Xiaofang1   

  1. 1. West China School of Public Health, NO.4 Hospital of West China, Sichuan University, Chengdu 610041, China;
    2. Suzhou Center for Disease Prevention and Control, Jiangsu Province, Suzhou 215004, China
  • Received:2015-04-19 Published:2016-01-26

摘要:

目的 :监测分析2011-2013年成都地区发热呼吸道症候群住院患儿鼻病毒(HRV)检出情况、病原型别及流行特征,为本地区HRV感染防控提供基础数据。 方法 :应用逆转录-聚合酶链式反应(RT-PCR)对2011年1月-2013年12月从1117例患儿鼻咽抽吸物标本提取的病毒核酸进行HRV-5'NCR筛检,随机挑取30例筛检阳性标本扩增HRV-VP4/VP2基因并测序,通过序列的比对分析鉴定病毒型别及变异状况。 结果 :1117份鼻咽抽吸物标本中HRV-5'NCR核酸检出率为24.62%(275/1117)。测序获得的30条HRV-VP4/VP2中,HRV-A型15例,HRV-B型4例,HRV-C型11例。检出高峰在11月,1岁以内儿童检出率最高;不同临床诊断疾病中,伴有喘息性疾病的患儿中HRV检出率最高。 结论 :HRV是成都地区呼吸症候群住院患儿病毒性感染的常见病原,HRV感染在成都地区全年均可发生,不同季节和年龄组检出率不同,呈现多个基因型共同流行且以A型为主的特点。

关键词: 鼻病毒, VP4/VP2基因, 发热呼吸道症候群, 逆转录聚合酶链反应, 基因型

Abstract:

Objective: To analyze the detection,etiological types and epidemic characteristics of human rhinovirus(HRV)in the hospitalized children with respiratory syndrome in Chengdu area during 2011—2013,and to provide basic data for prevention and control HRV infection.Methods: Altogether 1 117 nasopharyngeal aspirate samples were collected regularly from the children with respiratory syndrome. The samples were screened for the presence of RNA of HRV-5'NCR using RT-PCR.HRV specific sequence types and mutation status were identified by sequencing the VP4/VP2 coding region.Results: A total of 24.62%(275/1 117)of these samples were positive for HRV RNA.30 sequences were obtained,including 15 HRV-As,4 HRV-Bs and 11 HRV-Cs.The HRV detection peak was in November,and in different age groups,the detection rate for children under 1 years of age was higher than others.The highest detection rate of HRV was in the children with asthmatic diseases among different clinical diagnosis diseases.Conclusion: HRV is a common pathogen of viral infection in the hospitalized children with respiratory syndrome and it can be detected throughout the year in Chengdu area.The differences in detection rate between different genders and different seasons are satistically significant.Sequence analysis showed that multiple genotypes of rhinovirus are epidemic in common in Chengdu area and HRV-A is the main subtype.

Key words: rhinovirus, VP4/VP2 gene, respiratory syndrome, reverse transcriptase polymerase chain reaction, genotype

中图分类号: 

  • R373.2