多肿瘤标志物蛋白芯片检测在老年人健康体检中的应用价值及其临床意义

doi:10.13481/j.1671-587x.20160322

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (03): 535-540.doi: 10.13481/j.1671-587x.20160322

多肿瘤标志物蛋白芯片检测在老年人健康体检中的应用价值及其临床意义

李清茹¹, 季顺东^2,3

1. 苏州大学附属第一医院核医学科, 江苏苏州 215006;
2. 苏州大学附属第一医院江苏省血液研究所卫生部血栓与止血重点研究室, 江苏苏州 215006;
3. 苏州大学血液学协同创新中心, 江苏苏州 215006

收稿日期:2015-08-11 发布日期:2016-06-17
通讯作者: 季顺东,副研究员,硕士研究生导师(Tel:0512-67781380,E-mail:shundongji@163.com) E-mail:shundongji@163.com
作者简介:李清茹(1974-),女,江苏省苏州市人,副主任技师,主要从事临床标记免疫分析方面的研究。
基金资助:
国家自然科学基金面上项目资助课题(81472191);江苏省科技厅自然科学基金面上项目资助课题(BK20141203);江苏省政府科教兴卫工程-临床医学中心科研项目资助课题(ZX201102);江苏省血液病临床医学研究中心科研项目资助课题(江苏省科技厅生命健康专项-BL2012005)

Application of multiple tumor marker protein chip in healthy screening of old people and its clinical significance

LI Qingru¹, JI Shundong^2,3

1. Department of Nuclear Medicine, First Affiliated Hospital, Soochow University, Suzhou 215006, China;
2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, First Affiliated Hospital, Soochow University, Suzhou 215006, China;
3. Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, China

Received:2015-08-11 Published:2016-06-17

摘要/Abstract

摘要：

目的: 探讨多肿瘤标志物蛋白芯片技术(C12芯片法)在老年人健康体检中的应用,并量化分析不同年龄和性别体检者肿瘤标志物检测值和阳性率的差别,阐明其临床意义。方法: 选择健康体检者9654人,按年龄分为对照组(40~59岁)、老年组(60~79岁)和高龄组(80岁及以上)。采用多肿瘤标志物芯片检测系统检测各组体检者甲胎蛋白(AFP)、糖链抗原15-3(CA15-3)、糖链抗原19-9(CA19-9)、糖链抗原125(CA125)、糖链抗原242(CA242)、癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、铁蛋白(ferritin)、β-人绒毛膜促性腺激素(β-HCG)、人生长激素(HGH)、前列腺特异性抗原(PSA)和游离PSA(f-PSA)共12项肿瘤标志物水平,以超出正常范围为阳性结果,分析3组体检者样本的肿瘤标志物检测值和阳性率。结果: 1体检者9654人中共检出阳性结果665人,阳性检出率为6.89%,其中32人经内窥镜、病理学和影像学检查确诊为早期或中期肿瘤,肿瘤检出率为0.33％。2男女体检者肿瘤标志物阳性率不同,男性前5位依次为f-PSA、PSA、CEA、CA19-9和NSE;女性前5位依次为CA19-9、CA125、CEA、NSE和CA242。3CA19-9、CA242、CEA、NSE、PSA和f-PSA阳性率分布呈随年龄增长而升高的趋势(P<0.01)。4各组体检者AFP、CA125、CA19-9、CA242、CEA、HGH、NSE和PSA检测值随着年龄增长而升高,高龄体检者上述指标高于老年组和对照组(P<0.01),老年组上述指标高于对照组(P<0.01)。结论: C12芯片中CA19-9、CEA、NSE、PSA和f-PSA等肿瘤标志物在老年人、尤其是高龄老年人的健康筛查中阳性率高,有助于发现早期肿瘤。

关键词: 蛋白芯片, 多肿瘤标志物, 高龄老年人, 健康体检

Abstract:

Objective: To evaluate the role of the multiple tumor markers protein chip (C12 chip) in healthy screening of old people,and to quantitatively analyze the changes and clinical significance of the detection values and positive rates of tumor markers of the subjects with different ages and genders. Methods: A total of 9 654 cases were selected. According to the age,the subjects were divided into control group(aged 40 to 59),old people group(aged 60 to 79)and senile people group(aged 80 and above).The protein chip system for multiple tumor marker detection(Chip C-12) was used to measure the levels of 12 tumor markers including alpha fetoprotein(AFP),carcinoembryonic antigen(CEA),carbohydrate antigen 15-3(CA15-3),carbohydrate antigen 19-9(CA19-9),carbohydrate antigen 125(CA125),carbohydrate antigen 242(CA242),ferritin,human chorionic gonadotropin beta(β-HCG),human growth hormone(HGH),neuron specific enolase (NSE),prostate special antigen(PSA) and free PSA(f-PSA).The positive result was confirmed when the levels of one or more tumor markers were higher than the reference value.The detection values and the positive rates of the subjects in three groups were analyzed. Results: 1Among 9 654 cases,the serum levels of tumor biomarkers were found to be positive in 665 cases,and the positive rate was 6.89%;among them 32 cases were diagnosed as early or intermediate cancer through endoscope,pathological and imageological diagnosis,and the detection rate was 0.33%.2 The positive rates were different in different genders. In male,the top five order was f-PSA,PSA,CEA,CA19-9, and NSE;while in female,the top five order was CA19-9,CA125,CEA,NSE and CA242.3positive rates of CA19-9,CA242,CEA,NSE,PSA and f-PSA were increased with the increasing of age(P<0.01).4The values of AFP,CA125,CA19-9,CA242,CEA,HGH,NSE,and PSA were increased with the increasing of age; the above indexes of the subjects in senile people group were higher than those in old people group and control group(P<0.01),and the above indexes of the subjects in old group were higher than those in control group (P<0.01). Conclusion: The multiple tumor markers (CA19-9,CEA,NSE,PSA,and f-PSA) of C12 chip are useful in the healthy screening of the old people, especial in the senile people to discover tumor at an early stage.

Key words: protein chip, multiple tumor markers, senile people, healthy screening

中图分类号:

R446.61

李清茹, 季顺东. 多肿瘤标志物蛋白芯片检测在老年人健康体检中的应用价值及其临床意义[J]. 吉林大学学报(医学版), 2016, 42(03): 535-540.

LI Qingru, JI Shundong. Application of multiple tumor marker protein chip in healthy screening of old people and its clinical significance[J]. Journal of Jilin University Medicine Edition, 2016, 42(03): 535-540.

参考文献

[1] Torre LA,Bray F, Siegel RL,et al. Global cancer statistics,2012[J].CA Cancer J Clin, 2015,65(2):87-108.

[2] 刘险峰,唐俊.中国高龄老人的社会保障问题初探[J].中国行政管理,2011,6(3):20-23.

[3] Jimbo M, Rana GK, Hawley S,et al.What is lacking in current decision aids on cancer screening? [J].CA Cancer J Clin, 2013,63(3):193-214.

[4] Balachandra K,Laisupasin P,Dhepakson P,et al.Preliminary clinical evaluation of a protein chip for tumor marker serodiagnosis of various cancers [J].Asian Pac J Allergy Immunol,2003,21(3):171-178.

[5] Wingfield SA,Heflin MT.Cancer screening in older adults[J].Clin Geriatr Med, 2016,32(1):17-33.

[6] 严海燕,钟日辉,李竞,等.多肿瘤标志物蛋白芯片在老年人健康筛查中的应用[J].中华流行病学杂志,2011,32(9):924-926.

[7] 匡红,孙晨,李静,等.多肿瘤标志物蛋白芯片技术在人群健康体检中的应用[J].国际检验医学杂志,2013,34(9):1074-1076.

[8] 陆汉魁,季红,顾建英,等.多项肿瘤标志物生物芯片检测系统的初步临床应用[J].标记免疫分析与临床,2005,12(4):196-199.

[9] 林尤斌.肿瘤标志物蛋白芯片应用于老年人健康筛查的评估分析[J].中国老年学杂志,2013,33(12):2931-2932.

[10] 汪广杰,王东,张涛,等.血清多种肿瘤标志物联合检测对肺癌的诊断价值[J].重庆医学,2015,44(13):1759-1761.

[11] Center MM,Jemal A, Lortet-Tieulent J, et al.International variation in prostate cancer incidence and mortality rates[J].Eur Urol,2012,61(6):1079-1092.

[12] Tay KJ, Moul JW, Armstrong AJ.Management of prostate cancer in the elderly [J].Clin Geriatr Med,2016,32(1):113-132.

[13] Pomerantz M.Active surveillance:pathologic and clinical variables associated with outcome[J].Surg Pathol Clin,2015,8(4):581-585.

[14] 于普林,郑宏,苏鸿学,等.中国六城市老年人前列腺增生的患病率及相关因素[J].中华流行病学杂志, 2000,21(4):36-39.

[15] 邢金春.前列腺癌诊断治疗学[M].北京:人民卫生出版社,2011:65-67.

[16] Kravchick S,Peled R,Dorfman D,et al.Predictive criteria for prostate cancer detection in men with serum PSA concentration of 2.0 to 4.0 ng /ml[J]. Urology,2005,66(3):542-546.

多肿瘤标志物蛋白芯片检测在老年人健康体检中的应用价值及其临床意义

Application of multiple tumor marker protein chip in healthy screening of old people and its clinical significance

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

Metrics

本文评价

推荐阅读 10

[1]	李锐成, 郜赵伟, 张惠中, 林芳, 刘冲, 董轲. 胸腔积液中B7-H4蛋白联合ADA和CEA水平检测在胸腔积液鉴别诊断中的应用价值[J]. 吉林大学学报(医学版), 2020, 46(03): 614-619.
[2]	佟建秋，石炳毅，王禾，沈瑞雄，莫春柏，周文强. 口服供者脾细胞诱导成年大鼠心脏移植存活期延长[J]. J4, 2004, 30(2): 215-217.