非对称性二甲基精氨酸对豚鼠左心室流出道自律细胞电活动的影响

doi:10.13481/j.1671-587x.20170102

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (01): 6-10.doi: 10.13481/j.1671-587x.20170102

非对称性二甲基精氨酸对豚鼠左心室流出道自律细胞电活动的影响

赵兰平¹, 薛淑芳¹, 陈彦静², 王雪芳¹, 陈立锋¹, 黄嘉诚¹

1. 河北北方学院生理学教研室, 河北张家口 075000;
2. 中国中医科学院中医基础理论研究所, 北京 100700

收稿日期:2016-04-27 出版日期:2017-01-28 发布日期:2017-02-08
通讯作者: 赵兰平,教授,硕士研究生导师(Tel:0313-4029313,E-mail:zhaolp2009@126.com) E-mail:zhaolp2009@126.com
作者简介:赵兰平(1971-),女,河北省张家口市人,教授,医学博士,主要从事心脏电生理学方面的研究。
基金资助:
河北省教育厅高等学校科学技术研究重点项目资助课题（ZD20131054）；北方学院创新人才培育项目资助课题（CXRC1324）

Influence of ADMA in electrical activity of pacemaker cells in guinea-pig left ventricular outflow tract

ZHAO Lanping¹, XUE Shufang¹, CHEN Yanjing², WANG Xuefang¹, CHEN Lifeng¹, HUANG Jiacheng¹

1. Department of Physiology, Hebei North University, Zhangjiakou 075000, China;
2. Institute of Basic Theories of TCM, China Academy of Chinese Medical Sciences, Beijing 100700, China

Received:2016-04-27 Online:2017-01-28 Published:2017-02-08

摘要/Abstract

摘要：

目的：研究内源性一氧化氮合酶（NOS）抑制物非对称性二甲基精氨酸（ADMA）对豚鼠左心室流出道自律细胞电活动的影响，探讨ADMA在源于心室流出道心律失常发生发展中的作用。方法：制备豚鼠离体左心室流出道标本，用氧饱和的改良Locke液进行恒温、恒速灌流，采用标准玻璃微电极细胞内电位记录技术记录豚鼠离体左心室流出道部位的自发慢反应电位（sAP），观测该电位对ADMA的浓度依赖性效应，ADMA分为3个浓度组（1、10和100 μmol·L^-1）；用NO供体硝普钠（SNP）灌流预处理标本，分为对照组、SNP（10 μmol·L^-1）组和SNP+ADMA组，记录并分析SNP预处理对ADMA所致左心室流出道电生理效应的影响。结果：与对照组比较，1 μmol·L^-1ADMA组sAP各项指标均无明显变化（P>0.05），10和100 μmol·L^-1ADMA组4相自动去极速度（VDD）、自发放电频率（RPF）和0相最大除极速度（V_max）明显减慢（P<0.05），复极50%和90%时间（APD₅₀和APD₉₀）明显延长（P<0.05），100 μmol·L^-1ADMA组动作电位幅度（APA）明显减小（P<0.05）；与1 μmol·L^-1ADMA组比较，10 μmol·L^-1ADMA组RPF、APD₉₀及100 μmol·L^-1ADMA组RPF、V_max和APD₅₀差异有统计学意义（P<0.05）；与10 μmol·L^-1ADMA组比较，100 μmol·L^-1ADMA组APA明显减小（P<0.05）。10 μmol·L^-1 SNP预处理标本10min可逆转ADMA降低左心室流出道自发放电活动的效应，与对照组比较，VDD和RPF仍明显加快（P<0.05），V_max和APA有恢复至对照组水平的趋势（P>0.05），APD₉₀明显缩短（P<0.05）。结论：内源性NOS抑制物ADMA浓度依赖性降低左心室流出道自律细胞的自发放电活动，NO供体SNP预处理可逆转上述效应。

关键词: 非对称性二甲基精氨酸, 左心室流出道, 自发慢反应电位

Abstract:

Objective: To study the influence of endogenous nitric oxide synthase (NOS) inhibitor asymmetric dimethylarginine (ADMA) in the electrical activity of pacemaker cells in the guinea-pig left ventricular outflow tract,and to explore the effect of ADMA on the occurrence and development of ventricular outflow tract arrhythmias.Methods: The isolated guinea-pig left ventricular outflow tract tissue was perfused with fully oxygenated Locke solution under constant temperature and constant flow velocity.The spontaneous slow action potentials(sAP) of guinea-pig left ventricular tract were recorded by conventional intracellular microelectrode technique.The effects of NOS inhibitor ADMA(1,10 and 100 μmol·L^-1) on the spontaneous sAP were studied.And the effects of ADMA under the condition of pretreatment of sodium nitroprusside(SNP) were also explored(control group,10 μmol·L^-1 SNP group,and SNP+ADMA group).Results: Compared with control group,the different indexes of sAP in 1 μmol·L^-1 ADMA group had no significant changes(P>0.05).Compared with control group, the velocity of diastolic depolarization (VDD),the rate of pacemaker firing (RPF) and the maximal rate of depolarization(V_max) in 10 and 100 μmol·L^-1 ADMA groups were siginificantly decreased(P<0.05),and the 50% and 90% of duration of action potential(APD₅₀ and APD₉₀) were markedly increased(P<0.05). Compared with 1 μmol·L^-1ADMA group,the RPF in 10 μmol·L^-1 ADMA group was siginificantly decreased(P<0.05) and the APD₉₀ was markedly increased(P<0.05); compared with 1 μmol·L^-1ADMA group,the RPE,V_max,and APD₅₀ had significant changes (P<0.05).Compared with 10 μmol·L^-1ADMA group,the APA in 100 μmol·L^-1ADMA group was significantly decreased (P<0.05). After pretreatment of 10 μmol·L^-1 SNP for 10 min,followed by 10 μmol·L^-1 SNP and 10 μmol·L^-1 ADMA,the inhibitory effects of spontaneous activity induced by ADMA could be reversed;compared with control group,the VDD and RPF were significantly increased (P<0.05),the V_max and APA had no significant changes(P>0.05), and the APD₉₀ was markedly decreased (P<0.05).Conclusion: Endogenous NOS inhibitor ADMA can decrease the spontaneous activity of left ventricular outflow tract,and the inhibitory effects induced by ADMA can be reversed under the condition of pretreatment of NO donor SNP.

Key words: left ventricular outflow tract, spontaneous slow action potentials, asymmetric dimethylarginine

中图分类号:

Q463

赵兰平, 薛淑芳, 陈彦静, 王雪芳, 陈立锋, 黄嘉诚. 非对称性二甲基精氨酸对豚鼠左心室流出道自律细胞电活动的影响[J]. 吉林大学学报(医学版), 2017, 43(01): 6-10.

ZHAO Lanping, XUE Shufang, CHEN Yanjing, WANG Xuefang, CHEN Lifeng, HUANG Jiacheng. Influence of ADMA in electrical activity of pacemaker cells in guinea-pig left ventricular outflow tract[J]. Journal of Jilin University Medicine Edition, 2017, 43(01): 6-10.

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非对称性二甲基精氨酸对豚鼠左心室流出道自律细胞电活动的影响

Influence of ADMA in electrical activity of pacemaker cells in guinea-pig left ventricular outflow tract

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