红参发酵产物对高糖下大鼠肾小球系膜细胞增殖和细胞外基质降解的影响

doi:10.13481/j.1671-587x.20170207

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (02): 245-249.doi: 10.13481/j.1671-587x.20170207

红参发酵产物对高糖下大鼠肾小球系膜细胞增殖和细胞外基质降解的影响

曲萌¹, 董佳婧¹, 姜锐², 崔继春³, 孙坤洋⁴, 董志恒⁵, 孙立伟²

1. 北华大学基础医学院分子生物学教研室, 吉林吉林 132013;
2. 北华大学化学与生物学院吉林省中药生物技术创新中心, 吉林吉林 132013;
3. 吉化集团公司总医院门诊部, 吉林吉林 132021;
4. 吉林大学药学院实验药理与毒理学教研室, 吉林长春 130021;
5. 北华大学基础医学院病理学教研室, 吉林吉林 132013

收稿日期:2016-04-25 出版日期:2017-03-28 发布日期:2017-03-31
通讯作者: 董志恒,教授,硕士研究生导师(Tel:0432-64608106,E-mail:dzhqmdzh@aliyun.com);孙立伟,教授,硕士研究生导师(Tel:0432-62579125,E-mail:1017861999@qq.com) E-mail:dzhqmdzh@aliyun.com;1017861999@qq.com
作者简介:曲萌(1971-),女,吉林省吉林市人,副教授,医学博士,主要从事糖尿病慢性并发症方面的研究。
基金资助:
吉林省教育厅科研计划项目资助课题（吉教科合字[2016]第66号，吉教科合字[2014]第196号）；吉林省卫计委科研基金资助课题（2014Z082）；北华大学创新团队科研项目资助课题（20141225）；北华大学大学生创新训练项目资助课题（20160420）

Influence of fermented red ginseng extract in proliferation of rat glomerularmesangial cellsand extracellular matrix degradation under high sugar stimulation

QU Meng¹, DONG Jiajing¹, JIANG Rui², CUI Jichun³, SUN Kunyang⁴, DONG Zhiheng⁵, SUN Liwei²

1. Department of Molecular Biology, College of Basic Medical Sciences, Beihua University, Jilin 132013, China;
2. Jilin Province Chinese Medical Biotechnology Innovation Center, College of Chemistry and Biology, Beihua University, Jilin 132013, China;
3. Department of Outpatient, General Hospital, Jilin Chemical Industry Corporation, Jilin 132021, China;
4. Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University, Changchun 130021, China;
5. Department of Pathology, College of Basic Medical Sciences, Beihua University, Jilin 132013, China

Received:2016-04-25 Online:2017-03-28 Published:2017-03-31

摘要/Abstract

摘要： 目的：探讨红参发酵产物（FRGE）对高糖刺激下大鼠肾小球系膜细胞（GMCs）增殖和细胞外基质（ECM）降解的影响，阐明FRGE对糖尿病肾病（DN）的防治作用及可能机制。方法：将GMCs分为正常对照（NG）组、高糖（HG）组、高糖＋不同浓度（3.75、7.50和15.00 mg·L^-1）FRGE组。四甲基偶氮唑盐（MTT）光吸收法观察各组GMCs的增殖率，酶联免疫吸附法（ELISA）检测培养细胞上清中Ⅳ型胶原蛋白（Col Ⅳ）的水平，蛋白免疫印迹技术（Western blotting）检测基质金属蛋白酶2（MMP-2）和基质金属蛋白酶组织抑制物2（TIMP-2）蛋白表达水平。结果：与NG组比较，HG组GMCs增殖率升高（P<0.01），Col Ⅳ水平升高（P<0.01），TIMP-2蛋白表达水平上调（P<0.01），MMP-2蛋白表达水平下调（P<0.01）；与HG组比较，高糖＋不同浓度FRGE组GMCs增殖率均降低（P<0.01），Col Ⅳ水平降低（P<0.01），TIMP-2蛋白表达水平下调（P<0.01），MMP-2蛋白表达水平上调（P<0.01）。结论：FRGE可通过抑制高糖诱导的大鼠GMCs增殖，促进ECM的降解，延缓DN的发生发展。

关键词: 基质金属蛋白酶, 红参发酵产物, 糖尿病肾病, 细胞增殖, 肾小球系膜细胞

Abstract: Objective: To explore the influence of fermented red ginseng extract (FRGE) in the proliferation of rat glomerular mesangial cells (GMCs) and the degradation of extracellular matrix(ECM)under high sugar stimulation, and to clarify the prevention and treatment effects of FRGE on diabetic nephropathy ( DN) and the possible mechanism. Methods: The rat GMCs were cultured and divided into normal concentration of D-glucose (NG) group, high concentration of D-glucose (HG) group and high concentration of D-glucose plus different concentrations (3.75, 7.50, 15.00 mg·L^-1) of FRGE groups. The proliferation rates of rat GMCs were detected with MTT,and the type Ⅳcollagen(Col Ⅳ) levels in supernatants of the GMCs were detected by ELISA. The protein expressions levels of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) were detected with Western blotting method. Results: Compared with NG group, the proliferation rate of GMCs in HG group was increased(P<0.01), the Col Ⅳ level was increased(P<0.01),the MMP-2 expression level was decreased, and the TIMP-2 expression level was up-regulated(P<0.01). Compared with HG group, the proliferation rates of GMCs in various FRGE groups were decreased(P<0.01), the Col Ⅳ levels were decreased(P<0.01),the expression levels of TIMP-2 were reduced(P<0.01),and the expression levels of MMP-2 were increased(P<0.01). Conclusion: FRGE can inhibit the proliferation of rat GMCs induced by high sugar and promote the ECM degradation to delay the occurrence and development of DN.

Key words: matrix metalloproteinases, fermented red ginseng extract, diabetic nephropathy, cell proliferation, glomerular mesangial cells

中图分类号:

R587

曲萌, 董佳婧, 姜锐, 崔继春, 孙坤洋, 董志恒, 孙立伟. 红参发酵产物对高糖下大鼠肾小球系膜细胞增殖和细胞外基质降解的影响[J]. 吉林大学学报(医学版), 2017, 43(02): 245-249.

QU Meng, DONG Jiajing, JIANG Rui, CUI Jichun, SUN Kunyang, DONG Zhiheng, SUN Liwei. Influence of fermented red ginseng extract in proliferation of rat glomerularmesangial cellsand extracellular matrix degradation under high sugar stimulation[J]. Journal of Jilin University Medicine Edition, 2017, 43(02): 245-249.

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红参发酵产物对高糖下大鼠肾小球系膜细胞增殖和细胞外基质降解的影响

Influence of fermented red ginseng extract in proliferation of rat glomerularmesangial cellsand extracellular matrix degradation under high sugar stimulation

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