PLGA涂层处理的nHA-Mg多孔复合材料对兔颌骨缺损的修复作用

doi:10.13481/j.1671-587x.20170213

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (02): 276-280.doi: 10.13481/j.1671-587x.20170213

PLGA涂层处理的nHA-Mg多孔复合材料对兔颌骨缺损的修复作用

车鸿泽, 车彦海, 卢晴, 陈楠楠, 陈飞, 金权, 马宁

吉林大学口腔医院牙周病科, 吉林长春 130021

收稿日期:2017-01-05 出版日期:2017-03-28 发布日期:2017-03-31
通讯作者: 马宁,教授,硕士研究生导师(Tel:0431-88796039,E-mail:maningbsh76@sina.com) E-mail:maningbsh76@sina.com
作者简介:车鸿泽(1990-),男,吉林省长春市人,在读医学硕士,主要从事牙周病学诊治方面的研究。
基金资助:
吉林省发改委产业技术研究与开发项目资助课题（2013C022-5）

Repair effect of nHA-Mg porous composite materials modified by PLGA on jaw bone defect of rabbits

CHE Hongze, CHE Yanhai, LU Qing, CHEN Nannan, CHEN Fei, JIN Quan, MA Ning

Department of Periodontics, Stomatology Hospital, Jilin University, Changchun 130021, China

Received:2017-01-05 Online:2017-03-28 Published:2017-03-31

摘要/Abstract

摘要： 目的：观察表面经聚乳酸-羟基乙酸共聚物（PLGA）处理的纳米羟基磷灰石/镁（nHA-Mg）多孔复合材料植入到兔下颌骨骨缺损后的修复作用，并初步阐明其作用机制。方法：采用18只日本大耳白兔建立双侧下颌骨10 mm×5 mm×1 mm骨缺损动物模型，随机选取9只左侧植入表面经PLGA处理的nHA-Mg复合材料作为实验组，右侧不植入任何材料作为空白对照组；剩余9只左侧植入nHA-Mg复合材料作为阳性对照组，右侧同样作为空白对照组。于4、8和12周分次处死白兔（每次实验组和阳性对照组各3只），截取缺损区域下颌骨，进行影像学和组织学观察，并比较各组新生骨小梁面积占骨组织面积百分比以及实验组和阳性对照组材料的剩余量。结果：与阳性对照组和空白对照组比较，实验组新生骨小梁面积所占百分比明显升高（P<0.05），而阳性对照组与空白对照组比较差异无统计学意义（P>0.05）。影像学观察，实验组有新骨形成，并且效果明显优于阳性对照组和空白对照组。石蜡切片观察，实验组有血管组织和新生骨小梁，成骨细胞聚集在骨小梁周围，大量骨陷窝位于骨小梁中，陷窝中包含骨细胞；随时间延长，新生骨小梁增粗，排列更致密，有改建为板层骨趋势。硬组织磨片观察，实验组材料剩余量多于阳性对照组。结论：表面经PLGA处理的nHA-Mg多孔复合材料可以有效减缓体内的降解速率，并且材料具有促进成骨和引导骨再生的作用。

关键词: 聚乳酸-羟基乙酸共聚物, 纳米羟基磷灰石, 颌骨缺损, 镁

Abstract: Objective: To observe the repair effect of nano hydroxyapatite/magnesium(nHA-Mg)porous composite material modified by poly lactic acid/glycolic acid copolymer(PLGA) on the jaw bone defect of rabbits,and to elucidate the mechanisms preliminary. Methods: The mandibular defect models of 10 mm×5 mm×1 mm in the 18rabbits were established.Nine rabbits were selected and implanted with nHA-Mg composite materials modified by PLGA in the left as experimental group and the right as blank control group;the other nine were implanted with nHA-Mg in the left as positive control group and the right as blank control group.The rabbits were sacrificed at 4, 8, 12 weeks (respectively 3 of experimental group and positive control group every time)and the mandibular defect areas were intercepted and observed by imaging and histological examination;the sizes of the newborn trabecula area and residual materials in experimental group and positive control group were compared. Results: Compared with positive control group and blank control group, the percentage of newborn trabecula area of the rabbits in experimental group was increased (P<0.05),and there was no statistic difference between positive control group and blank control group (P>0.05).The imaging results showed that the new bone formation can be observed in experimental group and better than positive control group and blank control group.The paraffin section results indicated that there were visible vascular tissue and newborn trabecula,the osteoblasts gathered around the bone trabecula; a lot of pits were located in the bone trabecula, and the pits contained osteocytes in experimental group.As the prolongation of time,thickened newborn trabecula, dense arrangement and trend of converting to lamellar bone were observed in experimental group.The hard tissue section results showed that the remaining amount of materials in experimental group was more than that in positive control group. Conclusion: nHA-Mg porous composite materials modified by PLGA can effectively reduce the rate of degradation in the body, promote osteogenesis and guide the bone regeneration.

Key words: jaw bone defect, nano-hydroxyapatite, magnesium, poly lactic acid/glycolic acid

中图分类号:

R782.2

车鸿泽, 车彦海, 卢晴, 陈楠楠, 陈飞, 金权, 马宁. PLGA涂层处理的nHA-Mg多孔复合材料对兔颌骨缺损的修复作用[J]. 吉林大学学报(医学版), 2017, 43(02): 276-280.

CHE Hongze, CHE Yanhai, LU Qing, CHEN Nannan, CHEN Fei, JIN Quan, MA Ning. Repair effect of nHA-Mg porous composite materials modified by PLGA on jaw bone defect of rabbits[J]. Journal of Jilin University Medicine Edition, 2017, 43(02): 276-280.

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PLGA涂层处理的nHA-Mg多孔复合材料对兔颌骨缺损的修复作用

Repair effect of nHA-Mg porous composite materials modified by PLGA on jaw bone defect of rabbits

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