吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (04): 715-719.doi: 10.13481/j.1671-587x.20170410

• 基础研究 • 上一篇    下一篇

Notch 1对舌麟状细胞癌Tca8113细胞表皮生长因子受体表达的影响及其意义

李浩渤1, 杜勇2, 陈勇1, 李文静1   

  1. 1. 河北医科大学第二医院口腔内科, 河北 石家庄 050000;
    2. 河北省沧州市中心医院口腔科, 河北 沧州 061014
  • 收稿日期:2016-10-12 出版日期:2017-07-28 发布日期:2017-08-01
  • 通讯作者: 李浩渤,副主任医师(Tel:0311-66002729,E-mail:haobobrother12@163.com) E-mail:haobobrother12@163.com
  • 作者简介:李浩渤(1975-),男,河北省石家庄市人,副主任医师,医学硕士,主要从事牙周种植和牙体牙髓病诊治方面的研究。
  • 基金资助:
    河北省卫计委医学科学研究重点项目资助课题(20160101)

Influence of Notch 1 in expression of EGFR in tongue squamous cell carcinoma Tca8113 cells

LI Haobo1, DU Yong2, CHEN Yong1, LI Wenjing1   

  1. 1. Department of Oral Medicine, Second Hospital, Hebei Medical University, Shijiazhuang 050000, China;
    2. Department of Stomatology, Cangzhou Central Hospital, Hebei Province, Cangzhou 061014, China
  • Received:2016-10-12 Online:2017-07-28 Published:2017-08-01

摘要: 目的:将编码Notch 1胞內域(NICD)转染人舌麟状细胞癌(舌麟癌)Tca8113细胞,探讨Notch 1在Tca8113细胞中的作用及其对表皮生长因子受体(EGFR)表达的影响。方法:采用脂质体法将编码NICD的pRAMIC-IRES2-EGFP目的质粒和pIRES2-EGFP对照质粒分别转染人舌麟癌Tca8113细胞,实验分为目的质粒pRAMIC-IRES2-EGFP转染组、对照质粒pIRES2-EGFP转染组和非转染组。采用RT-PCR法、Western blotting法和免疫细胞化学法观察细胞中Notch 1mRNA及蛋白和EGFR mRNA及蛋白表达水平,MTT法检测Tca8113细胞的增殖活性并计算细胞存活率。结果:转染后,与非转染组和对照质粒pIRES2-EGFP转染组比较,pRAMIC-IRES2-EGFP目的质粒转染组Notch 1 mRNA和蛋白表达水平明显升高(P<0.05),而EGFR mRNA和蛋白表达水平明显降低(P<0.05),细胞存活率明显降低(P<0.05)。与非转染组比较,对照质粒pIRES2-EGFR转染组Notch 1 mRNA和蛋白表达水平、EGFR mRNA和蛋白表达水平及细胞存活率差异无统计学意义(P>0.05)。结论:Notch 1对舌麟癌Tca8113细胞增殖的影响可能与EGFR的表达下调有关。

关键词: 舌麟状细胞癌, Notch 1, 转染, 表皮生长因子受体

Abstract: Objective: To transfect the tongue squamous cell carcinoma Tca8113 cells with NICD, and to investigate the effects of Notch 1 on Tca8113 cells and the expression of epidermal growth factor receptor (EGFR) in tongue squamous cell carcinoma.Methods: The Notch plasmid pRAMIC-IRES2-EGFP encoding exogenous and control plasmid pIRES2-EGFP were transiently transfected into the Tca8113 cells by LipofectamineTM 2000. In the experiment, target plasmid pRAMIC-IRES2-EGFP transfection group, control plasmid pIRES2-EGFP transfection group and non-transfection group were set up. RT-PCR, Western blotting and immunocytochemical method were used to observe the mRNA and protein expression levels of Notch 1 and EGFR. The proliferation activities of Tca8113 cells were detected by MTT assay,and the survival rates were calculated.Results: Compared with non-transfection group and control plasmid pIRES2-EGFP transfection group, the mRNA and protein expression levels of Notch 1 in Tca8113 cells in pRAMIC-IRES2-EGFP group was significantly increased(P<0.05), the mRNA and protein expression levels of EGFR in Tca8113 cells were significantly decreased (P<0.05),and the proliferation rate of Tca8113 cells was significantly decreased(P<0.05) after transfection. There were no significant differences in the expression levels of Notch 1 and EGFR mRNA and protein and the proliferation rates in Tca8113 cells between non-transfection group and pRAMIC-IRES2-EGFP group (P>0.05).Conclusion: The effect of Notch 1 on the proliferation of Tca8113 cells may be related to down-ragulatoin of expression level of EGFR.

Key words: tongue squamous cell carcinoma, Notch 1, epidermal growth factor receptor, transfection

中图分类号: 

  • R739.86