吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (01): 101-105.doi: 10.13481/j.1671-587x.20180119

• 基础研究 • 上一篇    下一篇

血管内皮生长因子165b对人肝癌HepG2细胞生物学特性的影响

赵燕颖1, 王秀岩2, 秦国涛1, 孙远杰1, 刘志忠1, 李然伟2   

  1. 1. 吉林大学第四医院消化内科, 吉林 长春 130011;
    2. 吉林大学第二医院泌尿外科, 吉林 长春 130041
  • 收稿日期:2017-06-02 出版日期:2018-01-28 发布日期:2018-01-24
  • 通讯作者: 李然伟,主任医师,硕士研究生导师(Tel:0431-85909914,E-mail:zhaoyanying0609@126.com) E-mail:zhaoyanying0609@126.com
  • 作者简介:赵燕颖(1978-),女,吉林省长春市人,副主任医师,医学博士,主要从事消化内科相关疾病的临床诊治和基础方面的研究。
  • 基金资助:
    吉林省卫计委青年基金资助课题(2013Q024);吉林省科技厅自然科学基金资助课题(200905139)

Effect of VEGF165b on biological characteristics of human hepatocellular carcinoma HepG2 cells

ZHAO Yanying1, WANG Xiuyan2, QIN Guotao1, SUN Yuanjie1, LIU Zhizhong1, LI Ranwei2   

  1. 1. Department of Gastroenterology, Fourth Hospital, Jilin University, Changchun 130011, China;
    2. Department of Urinary Surgery, Second Hospital, Jilin University, Changchun 130041, China
  • Received:2017-06-02 Online:2018-01-28 Published:2018-01-24

摘要: 目的:探讨血管内皮生长因子165b(VEGF165b)对人肝癌HepG2细胞生物学特性的影响,并初步研究其作用机制。方法:HepG2细胞分为空白组(仅加转染试剂)、对照组(转染阴性对照PcDNA3.0表达载体)和PcDNA-VEGF165b组(转染VEGF165b表达载体PcDNA-VEGF165b)。MTT法检测各组HepG2细胞生存率,RT-PCR法和Western blotting法检测HepG2细胞中VEGF165b和VEGF165 mRNA和蛋白表达水平,Transwell小室法检测HepG2细胞迁移能力。结果:与空白组比较,对照组HepG2细胞中VEGF165b和VEGF165 mRNA和蛋白表达水平无明显变化(P>0.05)。与空白组比较,PcDNA-VEGF165b组细胞中VEGF165b mRNA和蛋白表达水平明显升高(P<0.05),VEGF165 mRNA和蛋白表达水平明显降低(P<0.05)。空白组和对照组细胞生存率比较差异无统计学意义(P>0.05);与空白组和对照组比较,PcDNA-VEGF165b组细胞生存率有所降低,但差异无统计学意义(P>0.05)。细胞迁移实验,空白组和对照组细胞迁移率比较差异无统计学意义(P<0.05);与空白组和对照组比较,PcDNA-VEGF165b组HepG2细胞迁移率明显降低(P<0.05)。结论:VEGF 165b能抑制VEGF165基因和蛋白的表达,VEGF165b对肝癌细胞增殖无明显影响,但可降低肝癌细胞的迁移能力。

关键词: 细胞迁移, 血管内皮细胞生长因子165, 细胞增殖, 血管内皮细胞生长因子165b, 肝肿瘤, HepG2细胞

Abstract: Objective: To study the effect of vascular endothelial growth factor 165b (VEGF165b) on the biological characteristics of human hepatocellular carcinoma HepG2 cells,and to explore its mechanism. Methods: The HepG2 cells were divided into blank group (treated with transfection reagent),control group(transfected with pcDNA3.0 expression vector)and pcDNA-VEGF165b group(transfected with pcNDA-VEGF165b).MTT assay was used to detect the survival rate of HepG2 cells;RT-PCR and Western blotting method were used to detect the expression levels of VEGF165b and VEGF165 mRNA and protein in the HepG2 cells;Transwell chamber assay was used to measure the migration ability of HepG2 cells. Results: Compared with blank group,there was no significant changes in the expression levels of VEGF165b and VEGF165 mRNA and protein in the HepG2 cells in control group(P>0.05).Compared with blank group,the expression levels of VEGF165b mRNA and protein in the HepG2 cells in PcDNA-VEGF165b group were significantly increased (P<0.05),and the expression levels of VEGF165 mRNA and protein were significantly decreased(P<0.05).There was no significant difference in the survival rates between blank group and control group (P>0.05).The survival rate of HepG2 cells in PcDNA-VEGF165b group was lower than those in blank group and control group,but the differences were not statistically significant (P>0.05).The cell migration experiment results showed that there was no significant difference in the migration rate between blank group and control group (P<0.05).Compared with blank group and control group,the migration rate of HepG2 cells in PcDNA-VEGF165b group was significantly reduced(P<0.05). Conclusion: VEGF165b can inhibit the expressions of VEGF165 mRNA and protein,and VEGF165b has no effect on the proliferation of hepatocellular carcinoma cells; but it can reduce the migration of hepatocellular carcinoma cells.

Key words: liver neoplasms, vascular endothelial growth factor 165, cell migration, HepG2 cells, vascular endothelial growth factor 165b, cell proliferation

中图分类号: 

  • R735.7