白芷总香豆素联合白芷挥发油对大鼠偏头痛的预防作用及其机制

doi:10.13481/j.1671-587x.20180306

摘要/Abstract

摘要： 目的：观察白芷总香豆素联合白芷挥发油对硝酸甘油诱导大鼠偏头痛的预防作用，并探讨其作用机制。方法：56只Wistar大鼠随机分为正常对照组（生理盐水0.1 mL·10 g^-1），偏头痛模型组（生理盐水0.1 mL·10 g^-1），白芷香豆素组（100 mg·kg^-1），白芷挥发油组（100 mg·kg^-1），低（25 mg·kg^-1）、中（50 mg·kg^-1）和高剂量（100 mg·kg^-1）白芷总香豆素和挥发油组合物组（组合物组）；每组8只。各组大鼠连续灌胃给药7 d，皮下注射硝酸甘油（10 mg·kg^-1）诱导大鼠偏头痛模型。观察各组大鼠行为表现，检测各组大鼠血清及脑组织中一氧化氮（NO）、血浆中降钙素基因相关肽（CGRP）和内皮素（ET）水平。结果：与正常对照组比较，偏头痛模型组大鼠甩头、前肢挠头和后腿拍脸次数增加（P<0.05或P<0.01），血清及脑组织中NO水平、血浆中CGRP和ET水平明显升高（P<0.05或P<0.01）。与偏头痛模型组比较，白芷总香豆素、白芷挥发油、各剂量组合物组大鼠甩头、前肢挠头和后腿拍脸次数减少（P<0.05或P<0.01），血清和脑组织中NO水平降低（P<0.05或P<0.01）。与偏头痛模型组比较，白芷挥发油和中、高剂量组合物组大鼠血浆中CGRP和ET水平降低（P<0.05）。结论：白芷2种活性成分总香豆素和挥发油组合物对硝酸甘油诱导的大鼠偏头痛具有一定的预防作用，且其作用机制可能与调节血管活性物质水平和功能有关联。

关键词: 白芷总香豆素, 白芷挥发油, 血管活性物质, 硝酸甘油, 偏头痛

Abstract: Objective: To observe the preventive effects of combined application of total coumarin and essential oil of Angelicae Dahuricae Radix on the migraine rats induced by nitroglycerin,and to explore their mechanisms. Methods: A total of 56 Wistar rats were randomly divided into normal control group (saline 0.1 mL·10 g^-1),migraine model group (saline 0.1 mL·10 g^-1),total coumarin of Angelicae Dahuricae Radix group(100 mg·kg^-1),essential oil of Angelicae Dahuricae Radix group(100 mg·kg^-1),and low dose (25 mg·kg^-1),middle dose(50 mg·kg^-1)and high dose(100 mg·kg^-1)of total coumarin and essential oil composition of Angelicae Dahuricae Radix groups(composition groups)(n=8).After continuously intragastric adminstration for 7 d,the rats were injected subcutaneously with nitroglycerin (10 mg·kg^-1) for establishing the migraine models.The behavior of the rats were observed,the levels of nitric oxide (NO) in serum and brain tissue of rats,the levels of calcitonin gene related peptide (CGRP) and endothelin (ET) in plasma of the rats in various groups were detected. Results: Compared with normal control group,the frequencies of head shaking,the face shoting times of posterior legs and the scratching times of anterior limbs of the rats in migraine model group were increased(P<0.05 or P<0.01),and the levels of NO in serum and brain tissue of the rats were increased(P<0.01).Compared with migraine model group,the frequencies of head shaking,the face shoting times of posterior legs and scratching times of anterior limbs of the rats in total coumarin group,essential oil group, and composition groups were decreased (P<0.05 or P<0.01),and the levels of NO in serum and brain tissue of the rats were decreased (P<0.05 or P<0.01). Compared with migraine model group,the levels of CGRP and ET in plasma of the rats in essential oil group,total coumarin group and middle and high doses of composition groups were decreased(P<0.05). Conclusion: The composition of two active ingredients of Angelicae Dahuricae Radix, total coumarin and essential oil, has preventive effects in the migraine rats induced by nitroglycerin,and their mechanisms may be related to regulation of the levels and function of vasoactive substances.

Key words: total coumarin of Angelicae Dahuricae Radix, vasoactive substances, essential oil of Angelicae Dahuricae Radix, migraine, nitroglycerin

中图分类号:

R258.5

倪红霞, 王春梅. 白芷总香豆素联合白芷挥发油对大鼠偏头痛的预防作用及其机制[J]. 吉林大学学报(医学版), 2018, 44(03): 487-492.

NI Hongxia, WANG Chunmei. Preventive effects of total coumarin and essential oil of Angelicae Dahuricae Radix on migraine rats and their mechanisms[J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 487-492.

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