吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (03): 537-541.doi: 10.13481/j.1671-587x.20150320

• 基础研究 • 上一篇    下一篇

正清风痛宁对偏头痛模型大鼠无菌性神经源性炎症反应的抑制作用

罗国标1,2, 杨丽1, 刘晓丽3   

  1. 1. 遵义医学院附属医院神经内科, 贵州 遵义 563099;
    2. 贵州省黔东南州人民医院神经内科, 贵州 凯里 556000;
    3. 遵义医学院附属医院病理科, 贵州 遵义 563099
  • 收稿日期:2014-09-20 发布日期:2015-08-01
  • 通讯作者: 杨丽,主任医师,硕士研究生导师(Tel:0852-8608747,E-mail:yangli1668@126.com) E-mail:yangli1668@126.com
  • 作者简介:罗国标(1977-),男,贵州省锦屏县人,主治医师,医学硕士,主要从事神经内科脑血管病和头痛基础和临床方面的研究。
  • 基金资助:

    贵州省省长基金项目资助课题(黔省专合字[2008]112号)

Inhibitory effect of Zhengqingfengtongning on aseptic neurogenic inflammation in migraine model rats

LUO Guobiao1,2, YANG Li1, LIU Xiaoli3   

  1. 1. Department of Neurology, Affiliated Hospital, Zunyi Medical University, Zunyi 563099, China;
    2. Department of Neurology, Qiandongnan People's Hospital, Guizhou Province, Kaili 556000, China;
    3. Department of Pathology, Affiliated Hospital, Zunyi Medical University, Zunyi 563099, China
  • Received:2014-09-20 Published:2015-08-01

摘要:

目的:观察偏头痛模型大鼠脑干中肿瘤坏子因子α(TNF-α)和白细胞介素1β(IL-1β)的表达及脑干蛛网膜下腔炎性细胞数的变化,探讨正清风痛宁对偏头痛无菌性神经源性炎症(ANI)的抑制作用。方法:60只健康Wistar大鼠随机分为空白对照组、模型组、舒马普坦组(6 mg·kg-1·d-1)、正清风痛宁低剂量组(100 mg·kg-1·d-1)、正清风痛宁中剂量组(150 mg·kg-1·d-1)和正清风痛宁高剂量组(200 mg·kg-1·d-1),每组10只。大鼠皮下注射硝酸甘油复制实验性偏头痛动物模型,药物干预4 h后断头取脑干及其脑膜。免疫组织化学法检测大鼠脑干中TNF-α和IL-1β的表达情况,显微镜下计数阳性细胞数;HE染色观察脑干蛛网膜下腔炎性细胞数,显微镜下计数蛛网膜下腔单位面积(以视野为单位)炎性细胞数。结果:与空白对照组比较,模型组大鼠脑干中TNF-α和IL-1β表达阳性细胞数明显增多,差异有统计学意义(P<0.01);与模型组比较,正清风痛宁中、高剂量组和舒马普坦组大鼠脑干中TNF-α和IL-1β表达阳性细胞数明显减少,差异有统计学意义(P<0.05)。与空白对照组比较,模型组大鼠脑干蛛网膜下腔炎性细胞(以淋巴细胞、单核细胞为主)数明显增多,差异有统计学意义(P<0.01)。与模型组比较,正清风痛宁低、中、高剂量组和舒马普坦组大鼠脑干蛛网膜下腔炎性细胞数明显减少,差异有统计学意义(P<0.01)。结论:正清风痛宁可抑制偏头痛模型大鼠的ANI,其在偏头痛治疗中有抗炎和镇痛作用。

关键词: 偏头痛, 正清风痛宁, 肿瘤坏子因子&alpha, 白细胞介素1&beta, 炎性细胞

Abstract:

Objective To observe the changes of the expressions of tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) in the brainstem and the number of inflammatory cells in brainstem subarachnoid of the rat models with migraine, and to explore the inhibitory effect of Zhengqingfengtongning on aseptic neurogenic inflammation(ANI) of the rats with migraine. Methods 60 healthy Wistar rats were randomly divided into blank control group, model group, sumatriptan(6 mg·kg-1·d-1) group, low dose of Zhengqingfengtongning(100 mg·kg-1·d-1) group, middle dose of Zhengqingfengtongning(150 mg·kg-1·d-1) group and high dose of Zhengqingfengtongning(200 mg·kg-1·d-1)group(n=10).The migraine rat model was established by subcutaneous administration of nitroglycerin.The brain and mater of the rats were decapitated 4 h after medical intervention.The expressions of TNF-α and IL-1β in the rat brainstem were evaluated by immunohistochemistry, and the number of TNF-α and IL-1β positive cells under microscope was counted.HE staining was used to observe the changes of the number of inflammatory cells in the brainstem subarachnoid of the rats and the number of inflammatory cells was counted under light microscope of per unit area subarachnoid space(in a view for unit). Results Compared with blank control group, the number of TNF-α and IL-1β expression positive cells in the brainstem of the rats in model group was increased significantly, and the differences were statistically significant(P<0.01);compared with model group, the number of TNF-α and IL-1β expression positive cells in the brainstem of the rats in middle and high doses of Zhengqingfengtongning groups and sumatriptan group was significantly decreased, the differences were statistically significant(P<0.05).Compared with blank control group, the number of inflammatory cells(mainly lymphocytes and monocytes) in the subarachnoid space of the rats in model group was significantly increased(P<0.01);compared with models group, the number of inflammatory cells in the subarachnoid space in different doses of Zhengqingfengtongning groups and sumatriptan group were significantly reduced(P<0.01). Conclusion Zhengqingfengtongning may inhibit the ANI of the rat model with migraine, suggesting that Zhengqingfengtongning has the effect of anti-inflammatory and analgesia in treatment of migraine.

Key words: migraine, Zhengqingfengtongning, tumor necrosis factor α, interleukin-1β, inflammatory cells

中图分类号: 

  • R285.5