机械敏感性离子通道蛋白Piezo在机体机械转导中作用的研究进展

doi:10.13481/j.1671-587x.20190345

摘要/Abstract

摘要： Piezo1和Piezo2是近年来新发现的机械敏感性离子通道，对机械门控非选择性阳离子电流的产生至关重要，在机械转导过程中发挥重要作用。本文系统地总结了国内外相关文献，重点论述Piezo通道在组织器官中的分布及表达，阐述其在机械转导途径中的作用，探讨其在不同组织器官中的表达及对机械转导作用的影响，为揭示Piezo基因相关的人类疾病探索新的路径。本文就Piezo家族对生物体生理功能的重要性及其与疾病的相关性进行介绍，主要概述Piezo通道的发现过程，总结Piezo1通道在血管、膀胱和肾脏等组织器官中的机械转导功能及Piezo2通道在触觉、本体感觉和伤害感觉等感觉系统中的机械转导功能，并介绍Piezo1和Piezo2突变体与多种人类疾病的关联，展望其作为重要基因或药物治疗靶点的前景。

关键词: 机械敏感性离子通道, Piezo1, Piezo2, 机械转导

中图分类号:

高瑞桐, 许林琪, 李峰. 机械敏感性离子通道蛋白Piezo在机体机械转导中作用的研究进展[J]. 吉林大学学报(医学版), 2019, 45(03): 725-730.

参考文献

[1] ANISHKIN A, LOUKIN SH, TENG J, et al. Feeling the hidden mechanical forces in lipid bilayer is an original sense[J]. Proc Natl AcadSci U S A, 2014, 111(22):7898-7905.
[2] NILIUS B, HONORÉ E. Sensing pressure with ion channels[J]. Trends Neurosci, 2012, 35(8):477-486.
[3] RANADE S S, SYEDA R, PATAPOUTIAN A. Mechanically activated ion channels[J]. Neuron, 2015, 87(6):1162-1179.
[4] COSTE B, MATHUR J, SCHMIDT M, et al. Piezo1 and piezo2 are essential components of distinct mechanically activated cation channels[J]. Science, 2010, 330(6000):55-60.
[5] COSTE B, XIAO B, SANTOS J S, et al. Piezos are pore-forming subunits of mechanically activated channels[J]. Nature, 2012, 483(7388):176-181.
[6] KIM S E, COSTE B, CHADHA A, et al. The role of drosophila Piezo in mechanical nociception[J]. Nature, 2012, 483(7388):209-212.
[7] KAMAJAYA A, KAISER J T, LEE J, et al. The structure of a conserved piezo channel domain reveals a topologically distinct β sandwich fold[J]. Structure, 2014, 22(10):1520-1527.
[8] FAUCHERRE A, NARGEOT J, MANGONI M E, et al. Piezo2b regulates vertebrate light touch response[J]. J Neurosci, 2013, 33(43):17089-17094.
[9] COSTE B, MURTHY S E, MATHUR J, et al. Piezo1 ion channel pore properties are dictated by C-terminal region[J]. Nat Commun, 2015, 6:7223.
[10] GE J, LI W, ZHAO Q, et al. Architecture of the mammalian mechanosensitive Piezo1 channel[J]. Nature, 2015, 527(7576):64-69.
[11] ZHAO QC, WU K, GENG J, et al. Ion permeation and mechanotransduction mechanisms of mechanosensitive Piezo channels[J]. Neuron, 2016, 89(6):1248-1263.
[12] VOLKERS L, MECHIOUKHI Y, COSTE B. Piezo channels:from structure to function[J]. Pflugers Arch, 2015, 467(1):95-99.
[13] WU J, LEWIS A H, GRANDL J. Touch, tension, and transduction - the function and regulation of Piezo ion channels[J]. Trends Biochem Sci, 2017, 42(1):57-71.
[14] LEE W, LEDDY H A, CHEN Y, et al. Synergy between Piezo1 and Piezo2 channels confers high-strain mechanosensitivity to articular cartilage[J]. Proc Natl Acad Sci U S A, 2014, 111(47):E5114-E5122.
[15] KHATIBI SHAHIDI M, KRIVANEK J, KAUKUA N, et al. Three dimensional imaging reveals new compartments and structural adaptations in odontoblasts[J]. J Dent Res, 2015, 94(7):945-954.
[16] FERRARI L F, BOGEN O, GREEN P, et al. Contribution of Piezo2 to endothelium-dependent pain[J].Mol Pain, 2015, 11:65.
[17] IKEDA R, CHA M, LING J, et al. Merkel cells transduce and encode tactile stimuli to drive Aβ-afferent impulses[J]. Cell, 2014, 157(3):664-675.
[18] MAKSIMOVIC S, NAKATANI M, BABA Y, et al. Epidermal Merkel cells are mechanosensory cells that tune mammalian touch receptors[J]. Nature, 2014, 509(7502):617-621.
[19] WOO S H, RANADE S, WEYER A D, et al. Piezo2 is required for Merkel-cell mechanotransduction[J]. Nature, 2014, 509(7502):622-626.
[20] WU ZZ, GRILLET N, ZHAO B, et al. Mechanosensory hair cells express two molecularly distinct mechanotransduction channels[J].Nat Neurosci, 2017, 20(1):24-33.
[21] WANG F, KNUTSON K, ALCAINO C, et al. Mechanosensitive ion channel Piezo2 is important for enterochromaffin cell response to mechanical forces[J]. J Physiol(Lond), 2017, 595(1):79-91.
[22] RANADE S S, QIU Z, WOO S H, et al. Piezo1, a mechanically activated ion channel, is required for vascular development in mice[J]. Proc Natl Acad Sci U S A, 2014, 111(28):10347-10352.
[23] LI J, HOU B, TUMOVA S, et al. Piezo1 integration of vascular architecture with physiological force[J]. Nature, 2014, 515(7526):279-282.
[24] RODE B, SHI J, ENDESH N, et al. Piezo1 channels sense whole body physical activity to reset cardiovascular homeostasis and enhance performance[J]. Nat Commun, 2017, 8(1):350.
[25] RETAILLEAU K, DUPRAT F, ARHATTE M, et al. Piezo1 in smooth muscle cells is involved in hypertension-dependent arterial remodeling[J]. Cell Rep, 2015, 13(6):1161-1171.
[26] MIYAMOTO T, MOCHIZUKI T, NAKAGOMI H, et al. Functional role for Piezo1 in stretch evoked Ca²⁺ influx and ATP release in urothelial cell cultures[J]. J Biol Chem, 2014, 289(23):16565-16575.
[27] MICHISHITA M, YANO K, TOMITA K I, et al. Piezo1 expression increases in rat bladder after partial bladder outlet obstruction[J]. Life Sci, 2016, 166:1-7.
[28] MARTINS J R, PENTON D, PEYRONNET R, et al. Piezo1-dependent regulation of urinary osmolarity[J]. Pflugers Arch, 2016, 468(7):1197-1206.
[29] PEYRONNET R, MARTINS J R, DUPRAT F, et al. Piezo1-dependent stretch-activated channels are inhibited by Polycystin-2 in renal tubular epithelial cells[J]. EMBO Rep, 2013, 14(12):1143-1148.
[30] BLUMENTHAL N R, HERMANSON O, HEIMRICH B, et al. Stochastic nanoroughness modulates neuron-astrocyte interactions and function via mechanosensing cation channels[J]. Proc Natl Acad Sci U S A, 2014, 111(45):16124-16129.
[31] JIN Y, LI J, WANG Y T, et al. Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells[J]. Angle Orthod, 2015, 85(1):87-94.
[32] GUDIPATY S A, LINDBLOM J, LOFTUS P D, et al. Mechanical stretch triggers rapid epithelial cell division through Piezo1[J].Nature, 2017, 543(7643):118-121.
[33] ABRAIRA V E, GINTY D D. The sensory neurons of touch[J]. Neuron, 2013, 79(4):618-639.
[34] ARNADÓTTIR J, CHALFIE M. Eukaryotic mechanosensitive channels[J]. Annu Rev Biophys, 2010, 39:111-137.
[35] RANADE S S, WOO S H, DUBIN A E, et al. Piezo2 is the major transducer of mechanical forces for touch sensation in mice[J]. Nature, 2014, 516(7529):121-125.
[36] SCHNEIDER E R, MASTROTTO M, LAURSEN W J, et al. Neuronal mechanism for acute mechanosensitivity in tactile-foraging waterfowl[J]. Proc Natl Acad Sci U S A, 2014, 111(41):14941-14946.
[37] PROSKE U, GANDEVIA S C. The proprioceptive senses:their roles in signaling body shape, body position and movement, and muscle force[J]. Physiol Rev, 2012, 92(4):1651-1697.
[38] WOO S H, LUKACS V, DE Nooij J C, et al. Piezo2 is the principal mechanotransduction channel for proprioception[J]. Nat Neurosci, 2015, 18(12):1756-1762.
[39] BORBIRO I, BADHEKA D, ROHACS T. Activation of TRPV₁ channels inhibits mechanosensitive Piezo channel activity by depleting membrane phosphoinositides[J]. Sci Signal, 2015, 8(363):ra15.
[40] YANG J, ZHANG J N, YANG H L, et al. The potential role of Piezo2 in the mediation of visceral sensation[J]. Neurosci Lett, 2016, 630:158-163.
[41] BAE C, GNANASAMBANDAM R, NICOLAI C, et al. Xerocytosis is caused by mutations that alter the kinetics of the mechanosensitive channel PIEZO₁[J]. Proc Natl Acad Sci U S A, 2013, 110(12):E1162-E1168.
[42] ALBUISSON J, MURTHY S E, BANDELL M, et al. Dehydrated hereditary stomatocytosis linked to gain-of-function mutations in mechanically activated PIEZO₁ ion channels[J]. Nat Commun, 2013, 4:1884.
[43] ANDOLFO I, ALPER S L, DE Franceschi L, et al. Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO₁[J]. Blood, 2013,121(19):3925-3935, S1-12.
[44] CAHALAN S M, LUKACS V, RANADE S S, et al. Piezo1 links mechanical forces to red blood cell volume[J]. Elife, 2015, 4:e07370.
[45] LUKACS V, MATHUR J, MAO R, et al. Impaired PIEZO₁ function in patients with a novel autosomal recessive congenital lymphatic dysplasia[J]. Nat Commun, 2015, 6:8329.
[46] FOTIOU E, MARTIN-ALMEDINA S, SIMPSON M A, et al. Novel mutations in PIEZO₁ cause anautosomal recessive generalized lymphatic dysplasia with non-immune hydrops fetalis[J].Nat Commun, 2015, 6:8085.
[47] MCMILLIN M J, BECK A E, CHONG J X, et al. Mutations in PIEZO₂ cause Gordonsyndrome, Marden-Walker syndrome, and distal arthrogryposis type 5[J]. Am J Hum Genet, 2014, 94(5):734-744.
[48] COSTE B, HOUGE G, MURRAY M F, et al. Gain-of-function mutations in the mechanically activated ion channel PIEZO2 cause a subtype of distalarthrogryposis[J]. Proc Natl Acad Sci U S A, 2013, 110(12):4667-4672.
[49] OKUBO M, FUJITA A, SAITO Y, et al. A family of distal arthrogryposis type 5 due to a novel PIEZO₂ mutation[J].Am J Med Genet A, 2015, 167A(5):1100-1106.