营养素复合物对酒精性肝病大鼠的保护作用及其机制

doi:10.13481/j.1671-587x.20190611

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1256-1260.doi: 10.13481/j.1671-587x.20190611

营养素复合物对酒精性肝病大鼠的保护作用及其机制

柴博文¹, 杨岳峰¹, 杨野仝², 周洋³, 张叶茁¹, 刘华锋¹, 刘雅娟¹

1. 吉林大学公共卫生学院营养与食品卫生学教研室, 吉林长春 130021;
2. 吉林省疾病预防控制中心, 吉林长春 130062;
3. 吉林省农业科学院, 吉林长春 130033

收稿日期:2018-11-29 出版日期:2019-12-05 发布日期:2019-12-05
通讯作者: 刘雅娟,教授,硕士研究生导师(Tel:0431-85619455,E-mail:1207209015@qq.com) E-mail:1207209015@qq.com
作者简介:柴博文(1993-),女,吉林省吉林市人,在读医学硕士,主要从事食品资源开发方面的研究。
基金资助:
吉林省科技厅科研基金资助课题（20160101236JC）

Protective effect of nutrient complex on alcoholic liver disease rats and its mechanism

CHAI Bowen¹, YANG Yuefeng¹, YANG Yetong², ZHOU Yang³, ZHANG Yezhuo¹, LIU Huafeng¹, LIU Yajuan¹

1. Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun 130021, China;
2. Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, China;
3. Jilin Provincial Academy of Agricultural Sciences, Changchun 130033, China

Received:2018-11-29 Online:2019-12-05 Published:2019-12-05

摘要/Abstract

摘要： 目的：探讨营养素复合物对酒精性肝病（ALD）大鼠的保护作用，并阐明其作用机制。方法：60只雄性Wistar大鼠随机分成空白对照组，模型对照组，低、中和高剂量（2.5、5.0和15.0 mL·kg^-1）营养素复合物组，每组12只。营养素复合物组大鼠灌胃不同剂量（2.5、5.0和15.0 mL·kg^-1）营养素复合物，其余2组大鼠给予等量蒸馏水；间隔2 h后，营养素复合物组和模型对照组大鼠给予30%白酒（15 mL·kg^-1），空白对照组大鼠给予等量蒸馏水，各组大鼠每天灌胃2次，持续36 d。取各组大鼠肝组织，测定肝组织中丙二醛（MDA）、还原型谷胱甘肽（GSH）和甘油三酯（TG）水平，观察各组大鼠肝组织病理形态表现，苏丹Ⅲ染色观察各组大鼠肝组织和肝细胞内脂滴数及肝组织中脂肪分布情况，并对肝组织脂肪变性进行评分。结果：与空白对照组比较，模型对照组大鼠肝脏组织匀浆中MDA和TG水平明显升高（P<0.01），GSH水平明显降低（P<0.05）；与模型对照组比较，低、中和高剂量营养素复合物组大鼠肝组织匀浆中MDA和TG水平明显降低（P<0.01），中和高剂量营养素复合物组大鼠肝组织匀浆中GSH水平明显升高（P<0.05或P<0.01）。模型对照组大鼠肝组织和肝细胞中可见大量脂滴，细胞核为蓝色，呈肝脂肪变性病理改变。与空白对照组比较，模型对照组大鼠肝组织和肝细胞内脂滴数性明显增多，肝组织脂肪变性病理评分升高（P<0.05）；与模型对照组比较，中和高剂量营养素复合物组大鼠肝组织和肝细胞内脂滴数明显减少，肝组织脂肪变性病理评分降低（P<0.05）。结论：营养素复合物能够通过增加肝脏中抗氧化物质，减少过氧化产物，对ALD起到一定的保护作用。

关键词: 营养素复合物, 酒精性肝病, 丙二醛, 还原型谷胱甘肽, 甘油三酯

Abstract: Objective: To explore the protective effect of nutrient complex on the rats with alcoholic liver disease(ALD),and to elucidate its mechanism. Methods: A total of 60 male Wistar rats were randomly divided into blank control group,model control group,low,medium and high doses (2.5,5.0,and 15.0 mL·kg^-1)of nutrient complex groups,and there were twelve rats in each group. The rats in blank control and model control groups were fed with distilled water and the rats in other groups were fed with different doses (2.5,5.0,and 15.0 mL·kg^-1)of nutrient complexes. The rats in blank control group were fed with distilled water again and the rats in other groups were fed with 30% wine 2 h later. The rats in various groups were administrated by gavage twice a day for 36 d.The liver tissue of the rats in various groups were taken and the levels of malonaldehyde(MDA),reduced glutathione(GSH) and triglyceride(TG) in liver tissue of the rats in various groups were detected.The pathological morphology of liver tissue of the rats in various groups was observed, Sudan Ⅲ staining was used to observe the number of lipid droplets in liver tissue and liver cells and the distribution of hepatic fat of the rats in various groups,and the steatosis of liver tissue of the rats in various groups was scored. Results: Compared with blank control group,the levels of MDA and TG in liver tissue of the rats in model control group were significantly increased(P<0.01),and the level of GSH was significantly decreased(P<0.05); compared with model control group,the levels of MDA and TG in liver tissue of the rats in low,medium and high doses of nutrient complex groups were decreased significantly(P<0.01),and the levels of GSH in liver tissue of the rats in medium and high doses of nutrient complex groups were significantly increased(P<0.05 or P<0.01). Large amounts of lipid droplets were found in the liver tissue and liver cells of the rats in model control group,the nucleus was blue and presented the pathological changes of steatosis of liver tissue. Compared with blank control group,the number of lipid droplets in liver tissue and liver cells of the rats in model control group was increased,and the pathological score of steatosis of liver tissue was increased(P<0.05); compared with model control group,the number of lipid droplets in liver tissue and liver cells of the rats in medium and high doses of nutrient complex groups was significantly decreased,and the pathological scores of steatosis of liver tissue were decreased (P<0.05). Conclusion: Nutrient complexes play a protective role in ALD by increasing the levels of antioxidants and reducing the peroxidation products in the liver tissue.

Key words: nutrient complex, alcoholic liver disease, malonaldehyde, reducing glutathione, triglyceride

中图分类号:

R155.3

柴博文, 杨岳峰, 杨野仝, 周洋, 张叶茁, 刘华锋, 刘雅娟. 营养素复合物对酒精性肝病大鼠的保护作用及其机制[J]. 吉林大学学报(医学版), 2019, 45(06): 1256-1260.

CHAI Bowen, YANG Yuefeng, YANG Yetong, ZHOU Yang, ZHANG Yezhuo, LIU Huafeng, LIU Yajuan. Protective effect of nutrient complex on alcoholic liver disease rats and its mechanism[J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1256-1260.

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营养素复合物对酒精性肝病大鼠的保护作用及其机制

Protective effect of nutrient complex on alcoholic liver disease rats and its mechanism

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