DKK1蛋白对SBC-3细胞增殖、迁移和侵袭能力的促进作用及其机制

doi:10.13481/j.1671-587x.20190617

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1294-1298.doi: 10.13481/j.1671-587x.20190617

DKK1蛋白对SBC-3细胞增殖、迁移和侵袭能力的促进作用及其机制

陈文娟¹, 冯海波², 赵征¹, 曹培培³, 韩乐⁴

1. 陕西省肿瘤医院内三科, 陕西西安 710061;
2. 陕西省肿瘤医院头颈外科, 陕西西安 710061;
3. 陕西省西安市中心医院肿瘤科, 陕西西安 710003;
4. 陕西省肿瘤医院胸外科, 陕西西安 710061

收稿日期:2019-05-31 出版日期:2019-12-05 发布日期:2019-12-05
通讯作者: 韩乐,主治医师(Tel:029-85276440,E-mail:56283859@qq.com) E-mail:56283859@qq.com
作者简介:陈文娟(1984-),女,陕西省西安市人,主治医师,医学博士,主要从事肺癌转移分子机制方面的研究。
基金资助:
陕西省自然科学基金资助课题（2019JM-538）；陕西省生健委卫生健康科研项目资助课题（2018D032）

Effects of DKK1 protein on proliferation,migration and invasion of SBC-3 cells and their mechanisms

CHEN Wenjuan¹, FENG Haibo², ZHAO Zheng¹, CAO Peipei³, HAN Le⁴

1. Department of Internal Medicine, Tumor Hospital, Shaanxi Province, Xi'an 710061, China;
2. Department of Head and Neck Surgery, Tumor Hospital, Shaanxi Province, Xi'an 710061, China;
3. Department of Oncology, Xi'an Central Hospital, Shaanxi Province, Xi'an 710003, China;
4. Department of Thoracic Surgery, Tumor Hospital, Shaanxi Province, Xi'an 710061, China

Received:2019-05-31 Online:2019-12-05 Published:2019-12-05

摘要/Abstract

摘要： 目的：探讨Dickkopf1（DKK1）蛋白对人小细胞肺癌（SCLC） SBC-3细胞生物学行为的影响，并阐明其机制。方法：过表达DKK1的慢病毒和对照病毒分别感染人SCLC细胞株SBC-3，获得稳定表达DKK1蛋白的细胞系（SBC-3-DKK1组）和对照细胞系（SBC-3-NC组）。采用RT-PCR和Western blotting法检测2组SBC-3细胞中DKK1 mRNA表达水平和蛋白表达量，平板克隆实验检测2组SBC-3细胞克隆形成率，Transwell实验观察2组SBC-3细胞迁移和侵袭能力，Western blotting法检测2组SBC-3细胞中MMP-9蛋白表达量。结果：慢病毒感染SBC-3细胞72 h后细胞内绿色荧光率大于80%。与SBC-3-NC组比较，SBC-3-DKK1组SBC-3细胞中DKK1 mRNA表达水平升高（P=0.004），DKK1蛋白表达量升高，细胞克隆形成率升高（P=0.0026），迁移细胞数增多（P=0.0062），侵袭细胞数增多（P=0.0214），SBC-3细胞中MMP-9蛋白表达量升高。结论：过表达DKK1蛋白可以促进SBC-3细胞的增殖、迁移和侵袭，其作有机制可能与上凋细胞中MMP-9蛋白表达有关。

关键词: 癌,小细胞肺, Dickkopf1, 基质金属蛋白9, 克隆形成率

Abstract: Objective: To investigate the effects of Dickkopf1(DKK1) protein on the biological behaviors of SBC-3 cells of human small cell lung cancer(SCLC),and to elucidate their mechanisms. Methods: The human SCLC cell strain SBC-3 were infected with lentiviruses over-expressing DKK1 and control viruses,respectively,and the cells stably expressing DKK1 protein (SBC-3-DKK1 group) and control cells (SBC-3-NC group) were obtained.RT-PCR and Western blotting methods were used to detect the expression levels of DKK1 mRNA and the expression amount of DKK1 protein in the SBC-3 cells in two groups;plate cloning assay was used to detect the colony formation rate of the cells in two groups;Transwell assay was used to observe the abilities of migration and invasion of the SBC-3 cells in two groups;Western blotting method was used to detect the expression amounts of MMP-9 protein in the SBC-3 cells in two groups. Results: The green fluorescence rate in the SBC-3 cells after lentivirus infection for 72 h was more than 80%.Compared with SBC-3-NC group,the expression level of DKK1 mRNA (P=0.004)and the expression amount of DKK1 protein in the SBC-3 cells in SBC-3-DKK1 group were significantly increased,the colony formation rate was significantly increased (P=0.002 6),the number of migration cells was significantly increased (P=0.006 2),the number of invadsion cells was significantly increased (P=0.021 4),and the expression amount of MMP-9 protein was significantly increased. Conclusion: Over-expression of DKK1 protein can promote the proliferation,migration and invasion of SBC-3 cells,and their mechanisms may be related to the up-regulation of MMP-9 protein expression.

Key words: cancer,small cell lung, dickkopf1, matrix metalloproteinase 9, colon formation rate

中图分类号:

R734.2

陈文娟, 冯海波, 赵征, 曹培培, 韩乐. DKK1蛋白对SBC-3细胞增殖、迁移和侵袭能力的促进作用及其机制[J]. 吉林大学学报(医学版), 2019, 45(06): 1294-1298.

CHEN Wenjuan, FENG Haibo, ZHAO Zheng, CAO Peipei, HAN Le. Effects of DKK1 protein on proliferation,migration and invasion of SBC-3 cells and their mechanisms[J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1294-1298.

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DKK1蛋白对SBC-3细胞增殖、迁移和侵袭能力的促进作用及其机制

Effects of DKK1 protein on proliferation,migration and invasion of SBC-3 cells and their mechanisms

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