吉林大学学报(医学版) ›› 2021, Vol. 47 ›› Issue (1): 173-179.doi: 10.13481/j.1671-587x.20210124

• 临床研究 • 上一篇    下一篇

EDC对牙本质即刻及老化微拉伸粘接强度的影响

沙青,李贺,张红,程博群,王莹,闫琳琳,邹馨颖,张志民()   

  1. 吉林大学口腔医院牙体牙髓科,吉林  长春  130021
  • 收稿日期:2020-04-20 出版日期:2021-01-28 发布日期:2021-01-27
  • 通讯作者: 张志民 E-mail:zhangzm1964@sina.com
  • 作者简介:沙 青(1994-),女,河南省郑州市人,在读硕士研究生,主要从事牙体牙髓病学方面的研究。
  • 基金资助:
    吉林省财政厅科技项目(jsz2018170-2)

Effects of EDC on immediate and aging micro-tensile bonding strength of dentin

Qing SHA,He LI,Hong ZHANG,Boqun CHENG,Ying WANG,Linlin YAN,Xinying ZOU,Zhimin ZHANG()   

  1. Department of Endodontics,Stomatology Hospital,Jilin University,Changchun 130021,China
  • Received:2020-04-20 Online:2021-01-28 Published:2021-01-27
  • Contact: Zhimin ZHANG E-mail:zhangzm1964@sina.com

摘要: 目的

探讨添加1-(3-二甲基氨基丙基)-3-乙基碳二亚胺(EDC)后通用型粘接剂的细胞毒性、微拉伸粘接强度和断裂模式,阐明EDC添加量对通用型粘接剂粘接性能的影响。

方法

将EDC添加到通用型粘接剂Single Bond Universal中,配制含不同浓度(0、0.1、0.2和0.3 mol·L-1)EDC的实验性粘接剂。对数生长期L-929成纤维细胞分为阴性对照组及0、0.1、0.2和0.3 mol·L-1 EDC组,并设空白对照组(培养液);阴性对照组细胞中加入培养液,不同浓度EDC组制备粘接剂膜并获取浸提液,将浸提液与细胞共培养,通过CCK-8法测定细胞相对增殖率(RGR),进行细胞毒性分级。选取新鲜拔除无龋的第三磨牙,制备微拉伸粘接试件,用万能试验机测试各组试件即刻(37℃去离子水中储存24 h)及老化(使用冷热循环机循环5 000次)微拉伸粘接强度,即刻微拉伸测试实验分为对照组(不含EDC)及0.1、0.2和0.3 mol·L-1 EDC组,老化微拉伸测试实验分为对照组(不含EDC)及0.1和0.2 mol·L-1 EDC组,用体式显微镜观察试件断裂模式。

结果

细胞毒性实验,各组细胞共培养24、48和72 h时RGR均在75%以上,细胞毒性为1级。即刻微拉伸测试实验,对照组和0.1、0.2及0.3 mol·L-1 EDC组粘接强度分别为(30.71±4.36)、(39.41±6.72)、(26.51±9.54)和(18.55±5.37)MPa, 与对照组比较,0.1 mol·L-1 EDC组粘接强度明显升高(P<0.05),0.3 mol·L-1 EDC组粘接强度明显降低(P<0.05);断裂模式,对照组及0.2和0.3 mol·L-1 EDC组以界面破坏为主,0.1 mol·L-1 EDC组以混合破坏为主。老化微拉伸测试实验,对照组及0.1和0.2 mol·L-1 EDC组粘接强度分别为(21.42±1.58)、(35.70±1.17)和(16.24±3.27)MPa,与对照组比较,0.1 mol·L-1 EDC组粘接强度明显升高(P<0.05),0.2 mol·L-1 EDC组粘接强度明显降低(P<0.05);断裂模式,对照组和0.2 mol·L-1 EDC组均以界面破坏为主,0.1 mol·L-1 EDC组以混合破坏为主。

结论

EDC添加到通用型粘接剂Single Bond Universal中配置的含0.1 mol·L-1 EDC的实验性粘接剂,无明显细胞毒性,即刻及老化微拉伸粘接强度均明显升高。

关键词: 碳二亚胺, 细胞毒性, 微拉伸粘接强度, 冷热循环

Abstract: Objective

To explore the the cytotoxicity, micro-tensile bond strength, and fracture mode of the universal adhesive after added with 1-ethyl-3 (3- dimethylaminopropyl) carbodiimide(EDC),and to clarify the effect of EDC addition amount on the bonding performance of universal adhesive.

Method

EDC was added to the universal adhesive Single Bond Universal to configure the experimental adhesives containing different concentrations (0.1,0.2 and 0.3 mol·L-1 ) of EDC.The L-929 fibroblasts in the logarithmic phase were divided into negative control group and 0,0.1,0.2 and 0.3 mol·L-1 EDC groups, and blank control group (culture medium) was set up;the L-929 cells in negative control were added with culture solution,and the adhesive film was prepared and the extract was obtained in different concentrations of EDC groups .The extract was co-cultured with the cells.The relative growth rate (RGR) was measured by CCK-8 method, and the cytotoxicity was graded.The freshly extracted third molars without caries were selected to prepare the micro-tensile bonding specimens. The universal testing machine was used to test the immediate(the specimens were stored in 37 ℃ deionized water for 24 h ) and aging(the specimens were treated in the hot and cold cycle machine for 5 000 times of circulation) micro-tensile bonding strengths of the specimens in various groups.The immediate micro-tensile test included control group (without EDC) and 0.1, 0.2 and 0.3 mol·L-1 EDC groups,and the aging micro-tensile test included control group (without EDC), and 0.1 and 0.2 mol·L-1 EDC groups.The fracture mode of the specimens was observed with a stereo microscope.

Results

In the cytotoxicity experiment, the RGR of cells in various group at 24, 48 and 72 h after co-cultivation were all above 75%,and the cytotoxicity was level 1. In the immediate micro-tensile test, the bonding strengths in control group and 0.1, 0.2 and 0.3 mol·L-1 EDC groups were (30.71±4.36), (39.41±6.72), (26.51±9.54) and(18.55±5.37) MPa.Compared with contol group,the bonding strength in 0.1 mol·L-1 EDC group was significantly increased (P<0.05),the bonding strength in 0.3 mol·L-1 EDC group was significantly reduced (P<0.05). The fracture mode observation results showed that the interface failure was the main performance in control group, 0.2 and 0.3 mol·L-1 EDC groups,and the mixed failure was the main performance in 0.1 mol·L-1 EDC group. In micro-tensile test,the bonding strengths in control group,0.1 mol·L-1 EDC group and 0.2 mol·L-1 EDC group were (21.42±1.58), (35.70±1.17), and (16.24±3.27) MPa, respectively, Compared with control group, the bonding strength in 0.1 mol·L-1 EDC group was significantly increased (P<0.05),and the bonding strength in 0.2 mol·L-1 EDC group was significantly reduced (P<0.05). The fracture mode observation results showed that the interface failure was the main performance in control group and 0.2 mol·L-1 EDC group, and the mixed failure was the main performance in 0.1 mol·L-1 EDC group.

Conclusion

The experimental adhesive containing 0.1 mol·L-1 EDC after added with EDC to the Single Bond Universal has no obvious cytotoxicity, and the immediate and aging micro-tensile bonding strengths are improved.

Key words: carbodiimide, cytotoxicity, micro-tensile bond strength, hot and cold cycles

中图分类号: 

  • R783.1