1-磷酸鞘氨醇对豚鼠心室肌细胞钙电流的影响

doi:吉林省科技厅资助课题

1-磷酸鞘氨醇对豚鼠心室肌细胞钙电流的影响

葛敬岩，赵春燕，张文杰，赵明，孙亚芹，钟国赣^*

吉林大学基础医学院生理学教研室，吉林长春130021

收稿日期:2004-04-13 修回日期:1900-01-01 出版日期:2005-01-28 发布日期:2005-01-28
通讯作者: 钟国赣

Effects of sphingosine-1-phosphate on calcium currentin isolated ventricular myocytes of guinea pig

GE Jing-yan, ZHAO Chun-yan, ZHANG Wen-jie, ZHAO Ming, SUN Ya-qin, ZHONG Guo-gan^*

Department of Physiology,School of Basic Medical Sciences,Jilin University,Changchun 130021, China

Received:2004-04-13 Revised:1900-01-01 Online:2005-01-28 Published:2005-01-28
Contact: ZHONG Guo-gan

摘要/Abstract

摘要： 目的：研究1-磷酸鞘氨醇（S1P）对心室肌细胞膜钙离子通道的作用及机制。方法：Langendorff离体心脏逆向灌流法分离豚鼠心室肌细胞，随机选取心室肌细胞分为正常对照组和S1P 0.1、1.0和10.0 μmol•L^-1剂量组以及百日咳毒素（PTX）0.1 mg•L^-1+S1P1.0 μmol•L^-1组。采用全细胞膜片钳技术分别记录各组心室肌细胞Ca²⁺电流。结果：1.0 μmol•L^-1的S1P使豚鼠心室肌细胞Ca²⁺电流从给药前的(0.256±0.030)mV增加到（0.367±0.090）mV（P<0.05），10.0 μmol•L^-1的S1P使Ca²⁺电流从给药前的(0.242±0.030)mV增加到(0.364±0.060)mV(P<0.01)，而加入G-蛋白耦联受体阻断剂PTX后，S1P对Ca²⁺通道电流的作用与给药前比较差异无显著性。结论：S1P通过与G-蛋白耦联的细胞膜受体介导促进豚鼠心室肌细胞Ca²⁺通道的开放,并具有一定的剂量依赖性。

关键词: 膜片钳术, 方法, 心室肌细胞, 钙通道, 百日咳毒素类

Abstract: Objective To study the effects of sphingosine-1-phosphate(S1P) on calcium current of ventricular myocytes and its mechanisms. Methods The Langendorff perfusion method was used to isolate the ventricular myocytes of guinea pig and the ventricular myocytes of guinea pig were randomly divided into one control group, three S1P groups with dosages of 0.1, 1.0, and 10.0 μmol•L^-1, and group of PTX 0.1 mg•L^-1 together with S1P 1.0 μmol•L^-1. The whole cell voltage clamp technique was used to record the calcium current of isolated ventricular myocytes of guinea pig. Results S1P 1.0 μmol•L^-1 enchanced the calcium current from (0.256±0.030)mV before administration to (0.367±0.090)mV after administration (P<0.05). S1P 10.0 μmol•L^-1 enchanced the calcium current from (0.242±0.030)mV before administration to (0.364±0.060)mV after administration（P<0.01）. The effects of S1P to enchance the calcium current was blocked by PTX, a G protein-coupled receptor blocking agent. Conclusion S1P can promote the opening of calcium channels of the ventricular myocytes by G protein-coupled membrane receptor in a dose-dependence manner.

Key words: patch clamp techniques, methods, ventricular myocytes, calcium channels, pertussis toxins

中图分类号:

Q463

葛敬岩，赵春燕，张文杰，赵明，孙亚芹，钟国赣. 1-磷酸鞘氨醇对豚鼠心室肌细胞钙电流的影响[J]. J4, 2005, 31(1): 52-54.

GE Jing-yan, ZHAO Chun-yan, ZHANG Wen-jie, ZHAO Ming, SUN Ya-qin, ZHONG Guo-gan. Effects of sphingosine-1-phosphate on calcium currentin isolated ventricular myocytes of guinea pig[J]. J4, 2005, 31(1): 52-54.

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