褪黑素对小鼠胸腺细胞电离辐射损伤的防护作用

doi:国家自然科学基金资助课题

褪黑素对小鼠胸腺细胞电离辐射损伤的防护作用

张萱，王珍琦，刘扬，龚守良^*，张铭，刘树铮

吉林大学公共卫生学院卫生部放射生物学重点实验室，吉林长春 130021

收稿日期:2002-09-05 修回日期:1900-01-01 出版日期:2004-11-28 发布日期:2004-11-28
通讯作者: 龚守良

Protective effects of melatonin on damage of thymocytesin mice induced by ionizing radiation

ZHANG Xuan, WANG Zhen-qi, LIU Yang, GONG Shou-liang^*, ZHANG Ming, LIU Shu-zheng

MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021

Received:2002-09-05 Revised:1900-01-01 Online:2004-11-28 Published:2004-11-28
Contact: GONG Shou-liang

摘要/Abstract

摘要： 目的：探讨外源性褪黑素（MLT）对电离辐射所致小鼠胸腺细胞损伤的影响及其机制。方法：通过腹腔注射方式给予昆明系小鼠外源性MLT，分别建立单次给药和连续给药两种动物模型。对于单次给药动物模型，腹腔注射不同浓度MLT后60 min给予1 Gy X射线全身照射，12 h后采用流式细胞术和荧光分光光度法分别检测胸腺细胞凋亡小体百分率和DNA裂解率的变化；对于连续给药动物模型，连续1周腹腔注射不同浓度MLT后给予1 Gy X射线全身照射，24 h后检测胸腺细胞数量及³H-TdR掺入率的变化。结果：单次给药动物模型，小鼠受1 Gy X射线全身照射后12 h，胸腺细胞数显著低于假照组（P<0.001），凋亡小体百分率和DNA裂解率显著高于假照组（P<0.001）。照射前预先给予外源性MLT，与0 mg•kg^-1 MLT组（单纯照射组）比较，胸腺细胞数增多，以0.5 mg•kg^-1 MLT组增多最明显（P<0.01）；凋亡小体百分率和DNA裂解率降低，在0.1~2.5 mg•kg^-1 MLT浓度范围内差异均具有显著性（P<0.001）。连续给药动物模型，小鼠受1 Gy X射线全身照射后24 h，胸腺细胞数及³H-TdR掺入率均显著低于假照组（P<0.001）。照射前预先给予外源性MLT，与单纯照射组相比，胸腺细胞数和³H-TdR掺入率均显著增多，前者在0.01~0.10 mg•kg^-1 MLT浓度范围内差异具有显著性（P<0.01），后者在0.1~1.0 mg•kg^-1 MLT浓度范围内差异均有显著性（P<0.05）。结论：照射前预先给予外源性MLT可减轻电离辐射所致小鼠胸腺细胞损伤，对小鼠免疫功能具有保护作用。

关键词: 辐射效应, 褪黑激素, 药理学, 辐射, 电离, 胸腺细胞, 辐射效应

Abstract: Objective To explore the effects of melatonin (MLT) on the damage of mouse thymocytes in vivo induced by ionizing radiation and its mechanism. Methods The exogenous MLT was given to Kunming mice to establish the animal models of single and successive administration of MLT through intraperitoneal injection before whole-body irradiation with 1 Gy X-rays. For single administration of MLT, the apoptotic body percentage(ABP) and DNA lytic rate(DLR) in the thymocytes were determined with flow cytometry and fluorospectrophotometry, respectively, 12 h after irradiation. For successive administration of MLT, ³H-TdR incorporative rate (HTIR) was determined 24 h after irradiation. Results The number of thymocytes in single adminstration group was significantly lower than that in the sham-irradiation group 12 h after irradiation with 1 Gy X-rays (P<0.001), while ABP and DLR increased significantly (P<0.001). When single administration of exogenous MLT was given before irradiation, the number of thymocytes in the 0.5 mg•kg^-1 MLT group was significantly higher, while the ABP and DLR were significantly lower than those in 0 mg•kg^-1 MLT group (simple irradiation, P<0.01 or P<0.001). The number of thymocytes and HTIR in adminsitration groups 24 h after irradiation were significantly lower than those in the sham-irradiation group (P<0.001). When successive administration of MLT was given for 1 week before irradiation, the numbers of thymocytes in adminsitration groups with 0.01~0.1 mg•kg^-1 MLT were significantly higher than that in 0 mg•kg^-1 MLT group (P<0.05), and the HTIR in 0.1~1.0 mg•kg^-1 MLT group was also significantly higher (P<0.05). Conclusion The administration of exogenous MLT before irradiation can decrease the damage of mouse thymocytes induced by ionizing radiation, and has the protective effect on immune functions in mice.

Key words: radiation effect, melatonin, pharmacology, ionizing radiation, thymocyte, radiation effect

中图分类号:

R144

张萱，王珍琦，刘扬，龚守良，张铭，刘树铮. 褪黑素对小鼠胸腺细胞电离辐射损伤的防护作用[J]. J4, 2004, 30(6): 897-900.

ZHANG Xuan, WANG Zhen-qi, LIU Yang, GONG Shou-liang, ZHANG Ming, LIU Shu-zheng. Protective effects of melatonin on damage of thymocytesin mice induced by ionizing radiation[J]. J4, 2004, 30(6): 897-900.

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