关键词: 单核细胞, 巨噬细胞, T细胞亚群

Abstract: Objective To study the effects of CD8⁺ T lymphocytes on processing macrophages (MΦ) of patients with asthma. Methods The MΦ and CD8⁺ T lymphocytes were isolated from peripheral blood of 20 patients with asthma and 22 health donors, respectively. Every sample was divided into four groups. All groups were stimulated with CTLL₂P antigen for 18 hours and CTLL₂P antigen were washed away, then they were cultured with B lymphocytes together. The levels of secreted CTLL₂P-specific IgM, IgG, and IgE in the supernatant were measured after 10 days. Results ①When MΦ processed antigen only, the level of CTLL₂P-specific IgM of patients with asthma（0.034±0.022） was lower than that of health donors (0.116±0.080) (P<0.05). The level of CTLL₂P-specific IgM of patients with asthma (0.031±0.021) was lower than that of health donors (0.079±0.064) (P<0.05)，when CD8⁺ T lymphocytes were cultured with mononuclear phagocytes together. ②When CD8⁺ T lymphocytes were cultured with mononuclear phagocytes together, the level of CTLL₂P-specific IgG of patients with asthma (0.102±0.041) was significantly higher than that of health donors (0.081±0.067) (P<0.05). However, the IgG levels had not differences when active CD8⁺ T lymphocytes or natural CD8⁺ T lymphocytes were cultured with antigen processing mononuclear phagocytes (0.105±0.066，0.079±0.059)，compared with health donors (0.066±0.038，0.069±0.047)(P>0.05). ③The CTLL₂P-specific IgE level of patients with asthma (0.171±0.154) was higher than that of health donors (0.147±0.059) (P<0.05), when CD8⁺ T lymphocytes were cultured with mononuclear phagocytes together to process antigen. Conclusion The CD8⁺ T lymphocytes of patients with asthma have different regulatory effect on immunolglobulin production from antigen processed by MΦ.

Key words: monocytes, macrophages, T-lymphocytes subsets