IFN-PDS合剂对流感病毒感染小鼠T淋巴细胞增殖反应性的影响

doi:吉林省科技发展计划非典重点项目

IFN-PDS合剂对流感病毒感染小鼠T淋巴细胞增殖反应性的影响

曹杰¹，孙连坤¹，程晓耕²，毛建华¹，刘喜春¹ ，王成²，李扬¹，赵雪俭^1*

1. 吉林大学基础医学院病理生理学教研室, 吉林长春130021；2. 长春生物制品研究所,吉林长春 130062

收稿日期:2003-12-20 修回日期:1900-01-01 出版日期:2004-05-28 发布日期:2004-05-28
通讯作者: 赵雪俭

Effects of IFN-PDS on T-lymphocyte proliferationin influenza virus-infected mice

CAO Jie¹, SUN Lian-kun¹, CHENG Xiao-geng², MAO Jian-hua¹,LIU Xi-chun¹, WANG Cheng², LI Yang¹, ZHAO Xue-jian^1*

1.Department of Phathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021,China;2.Insititute of Biological Products of Changchun, Changchun 130062,China

Received:2003-12-20 Revised:1900-01-01 Online:2004-05-28 Published:2004-05-28
Contact: ZHAO Xue-jian

摘要/Abstract

摘要： 目的：探讨人参二醇皂苷与干扰素（IFN-PDS）合剂对肺型流感病毒感染小鼠T淋巴细胞功能的影响。方法：清洁级昆明系小鼠60只，以流感病毒甲I型鼠肺适应株复制染毒模型。分为对照组（ConG）、染毒模型组（VIG）、PDS治疗组（PDSG）、IFN治疗组（IFNG）及IFN-PDS治疗组（IFN-PDSG）。应用MTT法测定脾和胸腺T淋巴细胞增殖功能。结果：①VIG脾指数和ConA诱导的脾、胸腺细胞增殖反应性明显地高于ConG (P<0.01、P<0.05)；② PDSG、IFNG和IFN-PDSG脾细胞自身增殖反应性，均显著地高于VIG（P<0.01、P<0.05和P<0.01），而PDSG、IFN-PDSG ConA诱导脾细胞增殖反应性显著低于VIG（P<0.05，P<0.01）；③ConA诱导胸腺细胞增殖反应性，PDSG、IFNG和IFN-PDSG均低于VIG（P<0.01、P<0.05、P<0.05）。结论：流感病毒可直接刺激脾、胸腺T淋巴细胞增殖，并明显地提高ConA诱导的T细胞增殖反应性； PDS、IFN-PDS增加流感病毒感染小鼠脾淋巴细胞自身增殖反应性，但对ConA诱导的脾、胸腺细胞过强的增殖反应性有抑制作用，显示对T淋巴细胞功能有双向调节作用。

关键词: 人参皂甙, 干扰素类, 流感病毒A型, T淋巴细胞

Abstract: Objective To investigate the effects of interferon-panaxadiol saponins (IFN-PDS) on the function of T-lymphocyte proliferation in influenza virus-infected mice. Methods 60 Kunming mice were divided into 5 groups: control group(ConG),influenza virus-infected group (VIG),PDS treatment group(PDSG),IFN treatment group (IFNG),IFN-PDS treatment group(IFN-PDSG). Mouse adopted influenza virus A was used to construct virus infected model. The functions of T lymphocyte proliferation in spleen and thymus gland were observed by MTT assay. Results ①The spleen index of VIG was much greater than that of ConG（P<0.01）. The mitogenesis induced by ConA of thymocyte and splenocyte was higher than that in ConG （P<0.05）. ②Self-proliferations of splenocytes in PDSG，IFNG, and IFN-PDSG were significantly higher than that in VIG（P<0.01,P<0.05, P<0.05）. The mitogenesis induced by ConA of splenocytes in PDS and IFN-PDSG were lower than that in VIG （P<0.05, P<0.01）.③The mitogenesis induced by ConA of thymocytes in PDSG,IFNG， and IFN-PDSG were lower than that in VIG （P<0.01,P<0.05, P<0.05）,had no difference compared with ConG（P>0.05）. Conclusion Influenza virus can stimulate the proliferation of spleen and thymic T lymphocyte directly. It can increase the T lymphocyte mitogenesis induced by ConA obviously. PDS and IFN-PDS treatment can increase the self-proliferation of splenocyte in influenza virus-infected mice, and decrease the excessive mitogenesis of splenocyte and thymocyte induced by ConA. It has two-way modulation on the function of T lymphocyte.

Key words: panaxoxides, interferons, influenza A virus, T lymphocytes

中图分类号:

R511

曹杰，孙连坤，程晓耕，毛建华，刘喜春，王成，李扬，赵雪俭. IFN-PDS合剂对流感病毒感染小鼠T淋巴细胞增殖反应性的影响[J]. J4, 2004, 30(3): 357-359.

CAO Jie, SUN Lian-kun, CHENG Xiao-geng, MAO Jian-hua,LIU Xi-chun, WANG Cheng, LI Yang, ZHAO Xue-jian. Effects of IFN-PDS on T-lymphocyte proliferationin influenza virus-infected mice[J]. J4, 2004, 30(3): 357-359.

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