关键词: 反义寡核苷酸, 顺铂, 神经胶质瘤U251细胞, 肿瘤浸润

Abstract: Abstract:Objective To investigate the effect of chloride channel ClC-2 antisense oligonucleotide (AON) on the cell invasion suppression of human glioma U251 cells induced by cisplatin (DDP).Methods The experiment was divided into four groups: control group ( nonsense oligonucleotide),AON group (ClC-2 antisense oligonucleotide),DDP group (cisplatin＋nonsense oligonucleotide),DDP＋AON group ( cisplatin＋ClC-2 antisense oligonucleotide).The mRNA expressions of ClC-2,cyclin D1,MMP-2 and MMP-9 of U251 cells were detected by RT-PCR.The invasion of U251 was measured by Transwell assay.Results Compared with control group,the mRNA expression rates of ClC-2,cyclin D1,MMP-2,MMP-9 in AON group,DDP group,DDP＋AON group were decreased （P＜0.05）.The numbers of invasion cells in control group,AON gr oup,DDP group and AON +DDP group were 81.00±4.58,42.01±4.36,48.33±2.52 and 12.67±5.13,respectively.Compared with control group,the numbers of invasion cells in AON group,DDP group and AON +DDP group were significantly decreased（P＜0.05）.Compared with control group,the rates of cell migration（%） in AON group,DDP group and AON +DDP group were 72.40±2.20,51.48±1.29 and 37.87±1.50,respectively.Cell migration of U251 cells was remarkably decreased after infected with ClC-2 antisense and treated with cisplatin（P＜0.05).Conclusion ①ClC-2 antisense oligonucleotide can inhibit the expression of ClC-2 mRNA in U251 cells. Inhibition of ClC-2 mRNA can suppress the mobility and invasion of U251 cells.③ClC-2 antisense oligonucleotide can facilitate invasion suppression of U251 cells induced by DDP.

Key words: antisense oligonucleotide, cisplatin, glioma U251 cells, tumor invasion