关键词: 胡桃醌, 顺铂, PI3K/AKt信号转导通路, 细胞凋亡, HeLa细胞

Abstract:

Objective: To explore the mechanism of promotion effect of juglone combined with cisplatin on the apoptosis of human cervical cancer HeLa cells, and to clarify the effects of its associated signal transduction pathways. Methods:The HeLa cells at logarithmic growth phase were divided into control group, juglone group, cisplatin group and juglone combined with cisplatin group (combined treatment group). The inhibitory rates of proliferation of HeLa cells were detected by MTT assay. The apoptosis was detected by Hoechst 33258 staining. The expressions of Bcl-2,Bax and caspase-3,AKt,and pAKt were detected by Western blotting method. Results: The MTT results showed that the HeLa cell proliferation at 24,48 72 h in each drug group was inhibited; compared with control group,the profileration of HeLa cells in juglone group and cisplatin group was significantly inhibited,especially in combined group.Compared with single drug group, the inhibitory effect in combined treatment group was more significantly. After treatment for 12 h, the typical morphological changes of apoptosis were found in juglone group and cisplatin group by Hoechst 33258 staining, especially in combined treatment group.The Western blotting results showed that the expression levels of Bcl-2 and pAKt in HeLa cells in juglone group and cisplatin group 12 h after treatment were decreased obviously,whereas the expression levels of Bax, Caspase-3, and AKt were increased significantly,especially in combined treatment group compared with control group. Conclusion: Juglone combined with cisplatin could inhibit the PI3K/AKt pathway, thereby promoting the apoptpsis of HeLa cells.

Key words: juglone, cisplatin, PI3K/AKt signal transduction pathways, apoptosis, HeLa cells