关键词: 糖尿病肾病；大鼠；内源性一氧化碳；血红素氧合酶-1；血红素氧合酶-2

Abstract:

Objective To observe the change of plasma carbon monoxide(CO) and the expressions of heme oxygenase-1(HO-1) and hemeoxygenase-2 (HO-2) in kidney tissues,and explore the effect of heme oxygenase/carbon monoxide(HO/CO)on development and progression of diabetic nephropathy. Methods 24 Sprague-Dawley rats were randomly divided into control group and model group,The rat models with diabetic nephropathy were established according to Anderson’ method. 8 weeks later,the urine of rats were collected to measure the 24 h urinary protein(Upro)and urinary creatinine(Ucr);the rats were sacrificed to measure the levels of CO,blood urea nitrogen(BUN),serum creatinine (Scr),meanwhile  the Ccr was calculated;the kidneys were harvested for observing the expressions of HO-1 and HO-2 by immunohistochemistry. Results The contents of Upro,BUN and Scrall were increased in model group,but Ccr and CO were decreased compared with control group(P<0.01);the content of plasma COin model group was obviously lower than that in control group(P<0.01);the expressions of HO-1 and HO-2 in kidney tissues were obviously reduced compared with control group (P<0.01). Conclusion The abnormal regulation of HO/CO is involved in the development and progression of diabetic nephropathy.

Key words: diabetic nephropathy;rat;carbon monoxide;heme oxygenase-1;heme oxygen