表皮生长因子受体单克隆抗体对辐射诱导肺腺癌细胞SPC-A-1的增敏作用

J4 ›› 2011, Vol. 37 ›› Issue (3): 483-486.

表皮生长因子受体单克隆抗体对辐射诱导肺腺癌细胞SPC-A-1的增敏作用

赵彤¹|王秀兰²|刘勇¹|王若雨¹

1.大连大学附属中山医院肿瘤科,辽宁大连 116001;
2.北京潞河医院消化内科|北京 101149

收稿日期:2011-01-05 出版日期:2011-05-28 发布日期:2011-05-28
通讯作者: 王若雨(Tel：0411-62893203，E-mail：wry1963@sohu.com) E-mail:wry1963@sohu.com
作者简介:赵彤（1964-）|女|吉林省吉林市人|副主任医师|医学硕士|主要从事肿瘤治疗的基础及临床研究。
基金资助:
辽宁省大连市卫生局科研基金资助课题（2008）

Radiosensitization of epithelial growth factor receptor monoclonal antibody on lung adenocarcinoma cell line SPC-A-1

ZHAO Tong¹,WANG Xiu-lan²|LIU Yong¹|WANG Ruo-yu¹

1.Department of Oncology,Affiliated Zhongshan Hospital of Dalian University,Dalian 116001,China;
2.Department of Gastroenterology,Luhe Hospital of Beijing,Beijing 101149,China

Received:2011-01-05 Online:2011-05-28 Published:2011-05-28

摘要/Abstract

摘要：

目的：探讨表皮生长因子受体单克隆抗体（C225）作为靶向治疗药物联合放射治疗对肺腺癌细胞系（SPC-A-1）的增敏作用，为临床综合治疗非小细胞肺癌提供理论依据。方法：SPC-A-1细胞体外传代培养6代以上，取对数生长期细胞进行实验。SPC-A-1细胞分为对照组（PBS）、照射组（4 Gy）、C225组（100 nmol·L^-1）和照射+ C225组（4Gy+100 nmol·L^-1），Hoechst 33258染色后采用荧光显微镜观察细胞凋亡情况。SPC-A-1细胞分别给予0、2、4和8 Gy 6-MV X射线照射后继续培养72 h，收集细胞，分为照射组和实验组（照射+C225组），用流式细胞仪检测细胞凋亡率。SPC-A-1细胞分为对照组（PBS）、C225组（100 nmol·L^-1）、照射组(8 Gy)和照射+ C225组（8　Gy+100 nmol·L^-1），作用48 h后，采用流式细胞仪检测细胞周期。结果：Hoechst 33258染色后，照射+ C225组凋亡细胞数明显高于照射组和C225组；细胞凋亡实验，实验组细胞凋亡率明显高于相应剂量照射组(P<0.05)。细胞周期检测,与对照组比较，C225组G0+G1期细胞增加(P<0.05)，照射组G2+M期细胞增加(P<0.05)，而照射+C225组G0+G1和G2+M期细胞均增加(P<0.05)；与对照组比较，C225组、照射组及照射+C225组S期细胞均下
降(P<0.05)。结论：C225对SPC-A-1细胞系有放射增敏作用，其机制可能与诱导细胞凋亡和细胞周期G0+G1期阻滞有关。

关键词: 表皮生长因子受体单克隆抗体；肺腺癌细胞株；放射治疗；放射敏感性

Abstract:

Objective To explore the radiosensitivity of C225(cetuximab)，an anti-epithelial growth factor receptor monoclonal antibody,which combined with irradiation against lung adenocarcinoma cell line SPC-A-1,and provide theoretical basis for clinical combined treatment in non-small lung cancer. Methods SPC-A-1 were cultivated in vitro for 6 passages,and the SPC-A-1 in logarithmic growth phase were seleted for experiment.The SPC-A-1 were divided into control group(PBS),irradiation group(4 Gy),C225 group(100 nmol·L^-1) and irradiation+C225 group(4 Gy+100 nmol·L^-1).The apoptosis of SPC-A-1 was observed by fluorescence microscope after Hoechst 33258 staining.SPC-A-1 were treated with different doses of 6-MV X-Rays including 0,2,4 and 8 Gy alone
or together with C225(100 nmol·L^-1),72 h after irradiation, the cells were divided into irradiation group and experimental group(irradiation+C22),the apototic rate was detected by flow cytometry (FCM).SPC-Ａ-1 were divided into control group(PBS),C225 group(100 nmol·L^-1),irradiation group(8 Gy) and irradiation+C225 group (8 Gy+100 nmol·L^-1), 48 h after irradiation, the cell cycle was determined by FCM.   Results A
fter staining by Hoechst 33258,the number of apoptotic cells in irradiation+C225 group was significantly higher than those in irradiation group and C225 group.In apoptosis experiment,the apoptotic rate in experimental group was higher than that in irradiation group(P<0.05).The cell cycle analysis showed that compared with control group,the number of cells in G0+G1 phase was increased in C225 group(P<0.05),the number of cells in G0+G1 and G2+M phrases in  irradiation+C225 group was increased(P<0.05),the number of cells in S phrase
of three groups was decreased(P<0.05). Conclusion The mechanism of radiosensitivity enhancement of C225 to SPC-A-1 may be related to arresting   G0+G1 phases and inducing apoptosis.

Key words: epithelial growth factor receptor monoclonal antibody;SPC-A-1 cell line;radiation therapy；radiosensitivity

中图分类号:

R73-3

赵彤, 王秀兰, 刘勇, 王若雨. 表皮生长因子受体单克隆抗体对辐射诱导肺腺癌细胞SPC-A-1的增敏作用[J]. J4, 2011, 37(3): 483-486.

ZHAO Tong, WANG Xiu-Lan, LIU Yong, WANG Ruo-Yu. Radiosensitization of epithelial growth factor receptor monoclonal antibody on lung adenocarcinoma cell line SPC-A-1[J]. J4, 2011, 37(3): 483-486.