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• 基础研究 • 上一篇    下一篇

红景天醇提取物对老龄小鼠肝细胞过氧化脂质及其凋亡的影响

宋祥福1,张静春2,吴晓辉1   

  1. 1.吉林大学公共卫生学院实验动物室,吉林 长春130021;2.吉林大学中日联谊医院检验科,吉林 长春130031
  • 收稿日期:2002-06-26 修回日期:1900-01-01 出版日期:2004-09-28 发布日期:2004-09-28

Effects of Rhodiola extracts on lipideperoxygenand apoptosis of hepatocytes in aged mice

SONG Xiang-fu1, ZHANG Jing-chun2, WU Xiao-hui1   

  1. 1. Department of Laboratory Animal, School of Pbulic Health, Jilin University, Changchun 130021,China;2. Department of Laboratory,China-Japan Union Hospital, Jilin University,Changchun 130031, China
  • Received:2002-06-26 Revised:1900-01-01 Online:2004-09-28 Published:2004-09-28

摘要: 目的:观察红景天醇提取物对肝细胞凋亡测定的作用。 方法:肝脏和血清过氧化脂质(LPO)测定分别采用硫代巴比妥酸法及磷钨酸法,细胞周期及细胞凋亡测定采用流式细胞术。 结果:红景天醇提取物显著降低小鼠肝及血清LPO含量,同时使细胞的高凋亡率显著下降。结论:红景天醇提取物具有抗肝细胞脂质过氧化及细胞凋亡的作用。

关键词: 过氧化脂质, 细胞凋亡

Abstract: Objective To observe the effects of Rhodiola extracts on hepatocytes apoptosis. Methods The levels of lipideperoxygen (LPO) in liver and serum were measured by thiobabituric acid (TBA) and phosphotungstic acid,respectively. The cell cycle and apoptosis were detected by flow cytometry (FCM). Results Rhodiola extracts significantly decreased the levels of LPO in liver and serum and the higher apopototic rate of hepatocytes.Conclusion Rhodiola extracts posses the effects of against lipid peroxidation and apoptosis.

Key words: lipideperoxygen, apoptosis

中图分类号: 

  • R-332