雄激素致不孕大鼠子宫转化生长因子β1及其受体的变化

doi:河北省科技厅科技攻关计划项目资助课题（06

雄激素致不孕大鼠子宫转化生长因子β1及其受体的变化

宋翠淼¹，杜惠兰²，段彦苍²，马惠荣²，李国明¹，张雪静¹

1.河北医科大学生理学教研室，河北石家庄　050091；2.河北医科大学中西医结合妇产科教研室，河北石家庄　050091

收稿日期:2006-09-28 修回日期:1900-01-01 出版日期:2007-07-28 发布日期:2007-07-28
通讯作者: 杜惠兰

Changes of transforming growth factor β1 and its receptor in uterus in androgen-induced sterile rats

SONG Cui-miao¹,DU Hui-lan²,DUAN Yan-cang²,MA Hui-rong²,LI Guo-ming¹,ZHANG Xue-jing¹

1.Department of Physiology,Hebei Medical University,Shijiazhuang 050091,China; 2.Department of Obstetrics and Gynecology of Intergrated Traditional Chinese Medicine and Western Medicine,Hebei Medical University,Shijiazhuang 050091,China

Received:2006-09-28 Revised:1900-01-01 Online:2007-07-28 Published:2007-07-28
Contact: DU Hui-lan

摘要/Abstract

摘要： 目的：研究雄激素致不孕大鼠（ASR）子宫转化生长因子β1（TGF-β1）及其受体（TGF-βRⅠ）表达量的变化。方法：出生9日龄SD雌性大鼠随机分为ASR模型组(n=12)及对照组(n=10)。模型组大鼠一次性皮下注射丙酸睾丸酮(T)1.25 mg，建立ASR模型。正常组注射等量中性茶油。采用放射免疫分析法测定血清T水平，采用免疫组织化学方法检测子宫TGF-β1和 TGF-βRⅠ的表达量，采用HE染色观察子宫组织形态学变化。结果：与正常组比较，ASR模型组大鼠血清T水平明显增高（P<0.01)；子宫内膜、肌层TGF-β1表达量明显降低（P<0.01）；肌层TGF-βRⅠ表达量明显降低（P<0.05），而内膜TGF-βRⅠ表达量却明显升高（P<0.05）；与正常组比较，ASR模型组大鼠子宫肌层变薄，内膜萎缩，腺体减少。结论：ASR模型子宫内膜及肌层TGF-β1和TGF-βRⅠ表达量异常，损伤子宫内膜的分化和肌层的发育，影响胚胎的植入。这些变化可能是导致不孕的原因之一。

关键词: 女(雌性), 雄激素, 副作用, 转化生长因子β, 受体, 转化生长因子β, 子宫

Abstract: Abstract:Objective To observe the changes of transforming growth factor β1 (TGF-β1) and its receptor(TGF-βRⅠ) in the uterus in androgen-induced sterile rats(ASR).Methods Twenty two SD female rats of 9-day age were randomly divided into two groups:ASR model group(n=12) and control group(n=10).ASR model was established by injecting testoserone propionate into rats subcutaneously. At the same time,the rats in control group were injected with neutral tea oil with same volume.The level of serum testosterone(T) was determined by means of radioimmunoassay.The expressions of TGF-β1 and TGF-βRⅠin the uterus were detected by immunohistochemistry method.The morphologic changes in the uterus by HE staining were observed. Results Compared with control group,the level of serum testosterone in medol group was increased obviously(P<0.01),the expressions of TGF-β1 in the endometrium and myometrium were decreased significantly(P<0.01), the expression of TGF-βRⅠ in myometrium was decreased obviously(P<0.05),while the expression of TGF-βRⅠin endometrium was increased significantly(P<0.05). In model group ,myometrium of the uterus was tenuis,endometrium was atrophic and the number of glandular organs was decreased.Conclusion The expressions of TGF-β1/ TGF-βRⅠin endometria and myometrium in ASR are abnormal,the differentiation of endonetrium and the growth of myometrium are injuied.These changes might be one of the reasons to lead to infertility.

Key words: female, androgens, adverse effects, transforming growth factor beta, receptors, transforming growth factor beta, uterus

中图分类号:

R711.6

宋翠淼，杜惠兰，段彦苍，马惠荣，李国明，张雪静. 雄激素致不孕大鼠子宫转化生长因子β1及其受体的变化[J]. J4, 2007, 33(4): 708-711.

SONG Cui-miao,DU Hui-lan,DUAN Yan-cang,MA Hui-rong,LI Guo-ming,ZHANG Xue-jing. Changes of transforming growth factor β1 and its receptor in uterus in androgen-induced sterile rats[J]. J4, 2007, 33(4): 708-711.

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