吉林大学学报(医学版)

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兔VX2肝肿瘤多时间点MR增强扫描信号特点及其病理学表现

韩耀启,黄仲奎,龙莉玲,李文美,李理   

  1. (广西医科大学第一附属医院放射科,广西 南宁 530021)
  • 收稿日期:2014-05-23 出版日期:2014-11-28 发布日期:2015-01-18
  • 通讯作者: 黄仲奎 E-mail:(Tel:0771-5356231,E-mail:cjr.hzhk@vip.163.com)
  • 作者简介:韩耀启(1981-),男,山东省菏泽市人,主治医师,在读医学博士,主要从事腹部放射诊断方面的研究。
  • 基金资助:

     国家自然科学基金资助课题(81260214)

Multi-time DCE-MRI and pathological characteristics of rabbit model bearing liver VX2 tumor

HAN Yao-qi,HUANG Zhong-kui,LONG Li-ling,LI Wen-mei,LI Li   

  1. (Department of Radiology,First Affiliated Hospital,Guangxi Medical University,Nanning 530021,China)
  • Received:2014-05-23 Online:2014-11-28 Published:2015-01-18

摘要:

目的:通过多时间点MR成像监测兔VX2肝肿瘤,观察肿瘤生长特性及MR动态增强扫描信号特点,为后续VX2肝肿瘤非手术治疗效果MR评估提供可靠依据。方法:采用开腹直视下肝组织内包埋法建立10只兔VX2肝肿瘤模型。于建模后第2、3、4和5周进行MR检查,测量并计算各时间点肿瘤体积(V)和肿瘤增长率(TGR),观察MR增强扫描信号特点。所有实验兔处死后,肿瘤标本进行HE染色,光镜下观察肿瘤边缘、中央不同部位的癌细胞分布情况。结果:建模后第3周TGR[(404.16±114.64)%]明显高于第4周TGR[(223.49±65.90)%](t=3.417,P<0.05)。MR增强扫描图像,肿瘤境界清晰,根据肿瘤增强特点可分为3个部分,即边缘环形明显强化部分、中央无强化部分以及二者之间的轻度不均匀强化部分。HE染色病理观察,全部为鳞癌,肿瘤边缘癌细胞排列紧密,向内为癌细胞与坏死间隔分布,肿瘤中央为无结构的坏死部分。结论:兔VX2肝肿瘤在建模后第3周生长较迅速,MRI动态增强扫描图像能较好地反应其组织结构特性。

关键词:  , VX2;肝肿瘤;磁共振成像;动态增强MR成像

Abstract:

Abstract:Objective
To observe the growth characteristics and multi-time dynamic contrast-enhanced MR(DCE-MR) findings of rabbit VX2 liver tumor on MR imaging,and to provide a reliable basis for the values of MR in assessment of non-surgical treatment effect of rabbit VX2 liver tumor.Methods 10 rabbit VX2 liver tumor models were established by liver tissue embedding through laparotomy under direct vision  and DCE-MRI scaning was performed at the 2nd,3rd,4th and 5th week after establishment.The tumor volume (V) and tumor growth rate(TGR)  were calculated at different time points and the signal characteristics were analyzed on MR imaging;then all the rabbits were excuted and   HE staining were performed to observe the cancer cells in different places with microscope.Results The TGR at the  3rd week after establishment (404.16%±114.64%) was significantly higher than that at the  4th week (223.49%±65.90%)(t=3.417,P<0.05).The tumors with well-boundary were rim-like enhanced significantly and the central parts were not enhanced on DCE-MR imaging,and the parts between the edge and centre of tumors were heterogeneously mild enhanced.The HE staining results showed that 6 cases of tumor were squamous cell carcinoma and the cells were distributed densely in the edge,the cancer cells and necrosis were distributed equally in the parts between the edge and centre of tumors and the centre of tumors were necrosis parts.Conclusion The rabbit VX2 liver tumor grows rapidly at the 3rd week after establishment,and its histological features can be better reflected by DCE-MRI.

Key words: VX2, liver neoplasms, magnetic resonance imaging, dynamic contrast-enhanced MR imaging

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