关键词: 沙眼, 热休克蛋白65, 基因, 疫苗

Abstract: To evaluate the protective ability of fusion protein of Hsp65-MOMP-T-epitopes(H-ctm1) against C.trachomatis(Ct) genital tract infection .Methods Female C57BL/6 mice aged 6—8 weeks were divided into H-ctm1，heat-killed EBs(HK-EBs) and PBS group（28 mice in each group，9 for detection of the amount of Ct IFU in vaginal tract of mice; 10 for histopathology; 9 for analysis of hydrosalpinx situation）.They were immunized by H-ctm1，HK-EBs and PBS, respectively.7 d before inoculation with Ct through vagina，progesterone was administered to all the experimental mice by subcutaneous injection.The number of Ct shedding from genital tract，inflammation score of genital tract，and situation of hydrosalpinx were evaluated between the three groups. Results On the 3rd and 6th day after infection，there was no significant difference of the amount of Ct in vaginal tract of mice between H-ctm1 and HK-EBs immunized groups; however，they were lower than that in PBS-immunized group（P＜0.01）.On the 9th and 18th day afterinfection，the amount of Ct in vaginal tract in H-ctm1 immunized group was lower than that in HK-EBS immunized group（P＜0.01 or P＜0.05）.6 d after infection，the inflammation scores in H-ctm1 immunized mice and HK-EBs immunized mice were lower than that in PBS immunized mice（P＜0.01 or P＜0.05）.12 d after infection，there were significant differences of vaginal inflammation scores between H-ctm1 immunized mice and HK-EBS immunized mice or PBS immunized mice（P＜0.05 or P＜0.01）.Hydrosalpinx was not found in H-ctm1 or HK-EBS immunized mice 40 d after infection.In contrast，unilateral or bilateral hydrosalpinx was present in all nine PBS immunized mice. Conclusion Protective immune response can be induced in the mice immunized with H-ctm1 following the Ct infection of genital tract.H-ctm1 as a candidate vaccine can shorten the duration of Ct infection effectively，which is better than HK-EBs.

Key words: heat-shock proteins 65, genes, vaccines