关键词: 前列腺肿瘤, 细胞凋亡

Abstract: To investigate the regulatory effect of curcumin on proliferation inhibition and apoptosis in human prostate carcinoma PC-3M cells.Methods The PC-3M cells were divided into 4 treatment groups (10,20,30 and 40 μmol•L^-1 curcumin) and control group. The morphological alterations of PC-3M cells after treated for different time were observed by using inverse microscopy,the inhibition of cell proliferation was detected by MTT assay, the cell cycle phase was analyzed by flow cytometry,the apoptosis was inspected by acridine orange-ethidium bromide fluorescent staining method,the protein level of caspase-3 in carcinoma cells was observed by SP immunohistochemistry. Results After being treated with curcumin, PC-3M cells grew round and small obviously and were against the wall under the inverse microscope. with the increasing of the concentration of cucurmin and the elongation of the treated time,the PC-3M cell growth was inhibited,the inhibition of proliferation of PC-3M cells increased in a time-and dose- dependent manner（P<0.01）;partial cells presented the morphological changes of apoptosis under the fluorescent microscope,the apoptotic rates of the control group and 4 treatment groups were（9.83±1.53）%,（19.50±1.00）%,（24.83±2.52）%,（30.17±2.08）% and（38.50±2.65）%, respectively;the cell number in S and G₂/M phase were increased, the cell number in G₀/G₁ phase was decreased;with the increasing of concentration of curcumin,the caspase-3 expression was increased in a dose- dependent manner（P<0.01）. Conclusion Curcumin could significantly inhibit the growth of PC-3M cells, the induction of apoptosis through up-regulating caspase-3 is probably one of its molecular mechanisms.

Key words: prostatic neoplasms, apoptosis