关键词: 肉瘤180, 肿瘤坏死因子, 白细胞白介素2

Abstract: To discuss the inhibitory effects of Juglans mandshurica maxim extract(JMME) on S₁₈₀ mouse sarcoma and its immunity accommodation.Methods The mice bearing sarcoma in three groups(n=10)were aderminastered with JMME with doses of 0.023,0.045 and 0.090　g•kg^-1•d^-1by gavage. Positive and negative control groups were set up.The inhibitory rate of JMME on S₁₈₀ mouse sarcoma was detected. The cell culture was used to observe the inhibitory effect of JMME on the sarcoma, the influences of JMME on the tumor necrosis factor(TNF) activity and the interleukin-2(IL-2) luring level were observed with MTT. Results JMME had inhibitory effect on the sarcoma. The inhibitory rates in JMME groups with different does were 32%,43%,56%,respectively,there were significant differences compared with negative group（P<0.05）. The inhibitory rate of JMME on tumor cell cultivated in vitro (60.10%)was higher than that in control group（P<0.05）. The TNF activity and IL-2 luring level in JMME group were higher than those in control group（P<0.05）. Conclusion JMME has significantly inhibitory effect on S₁₈₀ mouse sarcoma.Its mechanism is associated with inhibition of the growth of the exosomatic tumor and regulation of immunity function of the body.

Key words: sarcoma 180, tumor necrosis factor, interleukin-2