关键词: 乙型肝炎；T细胞亚群；细胞因子

Abstract:

Abstract:Objective To study the T lymphoctye subsets and Th1/Th2 type cytokines levels in peripheral blood of  patients with hepatitis B infection at different stages and healthy people and its clinical significance and to provide experimental basis for diagnosis and  therapy of  the diseases. Methods The percentages of CD3+,CD4+ and CD8+ T lymphocytes among total lymphocytes in the peripheral blood of patients with HBV infection at different stages including 14 cases of moderate chronic hepatitis B(CHB), 7 cases of severe CHB,8 cases of severe hepatitis B and 5 cases of acute hepatitis B and 20 healthy controls were detected by flow cytometry. The levels of IL-2,IL-4 and IFN-γ in serum were detected by ELISA method.Results Compared with  control group,the levels of  CD3+,CD4+T lymphocytes and the levels of IL-2 and IFN-γ in the patients in acute hepatitis B group were increased significantly（P<0.05）；the levels of  CD3+ and CD8+T lymphocytes and the level of  IFN-γ in patients in severe hepatitis B group were higher （P<0.05）； the levels of IL-4 and IFN-γ in the patients in moderate and severe CHB groups were increased （P<0.05）.Conclusion Th1 cytokines might play a role in the development of hepatitis B and the increasing of Th2 cytokine may be related to the chronic process  in the patients with HBV infection.

Key words: hepatitis B；T lymphocyte subsets；cytokines