关键词: 牙龈卟啉单胞菌；重组细胞分裂蛋白质;丝状温度敏感蛋白质；GTPase活性；热稳定性

Abstract:

Abstract:Objective To investigate the thermostability of GTPase activity of cell division protein FtsZ in Porphyromonas gingivalis（Pg) and to discuss the biochemical properties of FtsZ, and to provide experimental basis for curing periodontal disease.Methods Malachite green assay was used to measure the  the GTPase activities of recombinant Wt PgFtsZ,ZΔC01(missing 73 residues from the C-terminus of PgFtsZ),ZΔC02(missing 128 residues from the C-terminus of PgFtsZ),ZΔC03(missing 177 residues from the C-terminus of PgFtsZ),and ZΔC04(missing 233 residues from the C-terminus of PgFtsZ).The GTPase activities of different kinds of proteins were detected  after the protein dealed with unboiling and boiling for 5 and 10 min.The values of GTPase activities were detected at A650nm.Results The GTPase activities of ZΔC04  boiled for 5 and 10 min were higher than those of unboiled(P<0.05).The GTPase activities of WtPgFtsZ,ZΔC01,ZΔC02,and ZΔC03  boiled for 5 min were higher than those of unboiled(P<0.05).But the GTPase activities of WtPgFtsZ,ZΔC01,ZΔC02,and ZΔC03  boiled for 10 min were similar to those of unboiled.Conclusion Pg FtsZ  is a kind of  protein with thermostability,and ZΔC04 missing 233 residues from the C-terminus has higher thermostability.

Key words: Porphyromonas gingivalis, recombinant cell division protein;filamentous temperature sensitive protein Z, GTPase activity, thermostability