ER81在白消安模型小鼠睾丸和附睾中的表达及其意义

J4 ›› 2012, Vol. 38 ›› Issue (2): 180-182.

ER81在白消安模型小鼠睾丸和附睾中的表达及其意义

金波|刘洋|车冠宇|孙艺学|张学明

(吉林大学畜牧兽医学院动物胚胎工程吉林省重点实验室|吉林长春 130062)

收稿日期:2011-12-21 出版日期:2012-03-28 发布日期:2012-03-28
通讯作者: 张学明 E-mail:（Tel: 0431-87836162,E-mail：zhangxuem@jlu.edu.cn）
作者简介:金波（1987－）|男|湖南省安乡县人|基础兽医学硕士|主要从事哺乳动物生殖干细胞研究。
基金资助:
国家自然科学基金资助课题（30771555）；吉林大学基本科研业务费资助课题（20090333）

Expressions of transcription factor ER81 in testis and epididymis of busulfan-treated mice |and their significances

JIN Bo,LIU Yang,CHE Guan-yu,SUN Yi-xue,ZHANG Xue-ming

（Jilin Province Key Laboratory of Animal Embryo Engineering,College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China）

Received:2011-12-21 Online:2012-03-28 Published:2012-03-28

摘要/Abstract

摘要：

目的：检测Ets家族转录因子ER81在白消安模型小鼠睾丸及附睾中的表达水平，探讨其对精原细胞增殖和分化的影响。方法：成年小鼠腹腔注射白消安10 mg•kg-1，分别在注射后第0、3、5、8、10和18 天取睾丸和附睾，半定量RT-PCR分析ER81 mRNA的相对表达量。结果：在睾丸组织内，与第0天比较，ER81的表达量在第5 天显著降低（P<0.01），随后基本恢复；在附睾组织内，与第0天比较，ER81表达量于第8 天显著降低（P<0.05），并随后恢复。结论：转录因子ER81可能对精原细胞的分化有调节作用。

关键词: Ets家族转录因子ER81；睾丸；附睾；白消安；小鼠

Abstract:

To investigate the expressions of Ets family transcription factor ER81 in testis and epididymis of busulfan-treated mice,and explore its function on the proliferation and differentiation of spermatogonia.Methods Busulfan peritoneal injection (10 mg•kg-1) was performed and mouse testis and epididymis were collected on the 0th,3rd,5th,8th,10th,18th days after injection, respectively.The mRNA expression levels of ER81 in samples were analyzed by semi-quantitative RT-PCR.Results Compared with the 0th day,the expression of ER81 in testis was significantly decreased on the 5th day (P<0.01) and then recovered gradually.In epididymis,compared with the 0th day,the expression of ER81 on the 8th day was significantly decreased(P<0.05).Conclusion ER81 may modulate the differentiation of spermatogonia.

Key words: Ets family transcription factor ER81;testis;epididymis;busulfan;mouse

中图分类号:

S 814.1

金波|刘洋|车冠宇|孙艺学|张学明. ER81在白消安模型小鼠睾丸和附睾中的表达及其意义[J]. J4, 2012, 38(2): 180-182.

JIN Bo,LIU Yang,CHE Guan-yu,SUN Yi-xue,ZHANG Xue-ming. Expressions of transcription factor ER81 in testis and epididymis of busulfan-treated mice |and their significances[J]. J4, 2012, 38(2): 180-182.