orexin-B细胞及其神经纤维在癫痫模型大鼠癫痫发作不同时间点的变化及其意义

J4 ›› 2012, Vol. 38 ›› Issue (2): 207-210.

orexin-B细胞及其神经纤维在癫痫模型大鼠癫痫发作不同时间点的变化及其意义

福顺|王卓|王湘庆|郎森阳

（解放军总医院神经内科|北京 100853）

收稿日期:2011-09-28 出版日期:2012-03-28 发布日期:2012-03-28
通讯作者: 郎森阳 E-mail:（Tel：010-66936519，E-mail: langsy@263.net）
作者简介:陈福顺（1979－）|男|福建省龙海市人|在读医学硕士|主要从事癫痫的基础与临床研究。
基金资助:
国家自然科学基金资助课题（30971025）

Changes of orexin-B cells and their nerve fibers in rat epilepsy model at different time points and their significances

CHEN Fu-shun,WANG Zhuo,WANG Xiang-qing,LANG Sen-yang

(Department of Neurology,General Hospital of PLA,Beijing 100853,China)

Received:2011-09-28 Online:2012-03-28 Published:2012-03-28

摘要/Abstract

摘要：

目的：观察癫痫模型大鼠orexin-B细胞及其神经纤维在致痫后不同时间点的变化，阐明其在癫痫发作中的作用。方法：用海人酸（KA）腹腔注射诱发大鼠癫痫发作，并分别于癫痫终止后8 h、1 d、3 d、7 d和慢性复发时间点采用免疫组化方法检测orexin-B免疫反应阳性细胞数及其神经纤维的变化。结果：orexin-B免疫阳性细胞主要分布在大鼠下丘脑和穹窿周围核，但是在海马有可疑的阳性，而其免疫阳性神经纤维分布广泛；orexin-B免疫阳性细脃数呈现为先稍微减少后恢复、之后减少再恢复的趋势；7 d组细胞数减少，与其他组比较差异有统计学意义（P<0.05），而其免疫反应神经纤维平均光密度值呈现出与其细胞数相反的变化。结论：orexin-B细胞数及其神经纤维在大鼠癫痫发生过程中有变化，其可能参与了癫痫的神经元放电和传播的调节，增加其浓度有助于对癫痫的控制。

关键词: 癫痫；orexin-B；大鼠；海人酸

Abstract:

Abstract:Objective
To observe the changes of the orexin-B cells and their nerve fibers in the rat epilepsy model at different time points and elucidate their roles in the seizure.Methods Seizures were induced by intraperitoneal injection of kainic acid(KA),then the changes of the orexin-B immunoreactive cells and nerve fibers were detected by immunohistochemistry at 8 h,1 d, 3 d,7 d and chronic relapsing time after the termination of epilepsy respectively.Results The orexin-B immunoreactive cells mainly distributed in the hypothalamus and perifornical nuclei,not cerebral cortex and other brain tissues,but the suspicious positive cells were detected in the hippocampus.The orexin-B immunoreactive nerve fibers distributed widely.The orexin-B immunoreactive cells were decreased slightly in the first and resumed，then were decreased and resumed again.And the cell count of orexin-B was significantly decreased in 7 d group compared with other groups(P<0.05).The change trend of cell count of orexin-B was opposite to the one of nerve fibers. Conclusion The orexin-B immunoreactive cells and the immunoreactive fibers of the orexin-B have changes in the process of the seizures,it shows that they may involved in the regulation of epilepsy.And it can help to control seizures by increasing the concentration of orexin-B.

Key words: epilepsy, orexin-B, rats, kainic acid

中图分类号:

R742.1

福顺|王卓|王湘庆|郎森阳. orexin-B细胞及其神经纤维在癫痫模型大鼠癫痫发作不同时间点的变化及其意义[J]. J4, 2012, 38(2): 207-210.

CHEN Fu-shun,WANG Zhuo,WANG Xiang-qing,LANG Sen-yang. Changes of orexin-B cells and their nerve fibers in rat epilepsy model at different time points and their significances[J]. J4, 2012, 38(2): 207-210.