关键词: 多态性, 单核苷酸, 基因, myc, 基因, ras

Abstract: Abstract:Objective To investigate the relation between polymorphism of c-myc and k-ras gene and thymic lymphomas of mice induced by ionizing radiation. Methods The thymic lymphomas models of mice were made by ⁶⁰Co(dose rate:0.382 Gy•min^-1， total dose: 7 Gy)exposuring to C57BL/6J and BALB/c,6 months later,the mice were killed and thymus tissues were taken, genome DNA of thymic lymphomas cell was extracted by Promega DNA purification kit, PCR-restriction fragment length polymorphism (RFLP) was used to examine the single nucleotide polymorphism（SNP）of rs51048361（C/T）of c-myc gene,rs30221756（A/G）and rs30161609（T/C）of k-ras gene. Results The thymic lymphomas incidence induced by ⁶⁰Co exposuring in two kinds of mice was different,the genotypes of rs51048361 site were C/C and T/T,the genotypes of rs30221756 site were A/A and G/G,the genotypes of rs30161609 site were T/T and C/C.Conclusion After ⁶⁰Co exposuring,the genotypes of rs51048361,rs30221756 and rs30161609 in C57BL/6J and BALB/c with different radiosusceptibility are different,so it is inferred that there is relevance between the polymorphism of c-myc and k-ras gene and thymic lymphomas induced by ionizing radiation.

Key words: polymorphism, single nucleotide, genes, myc, genes, ras