关键词: 1-磷脂酰肌醇3-激酶, 逆转录聚合酶链反应, 免疫组织化学

Abstract: To study the expression of phosphatidylinositol 3-kinase(PI3K) in ovarian cancer tissue and the role of PI3K in the occurrence and development of ovarian cancer.Methods RT-PCR method was used to investigate the expressions of PI3K mRNA in tissue samples of 10 cases of ovarian cancer,10 benign ovarian tumor and 10 normal ovary.The expressions of PI3K protein in 60 cases of ovarian cancer,24 ovarian benign tumor and 24 normol ovarian tissues were detected by immunohistochemistry. Results The level of PI3K mRNA expression in ovarian cancer tissues was significantly higher than those in normal ovarian tissues and benign ovarian tumor tissues（P<0.01）,and there was no difference between benign ovarian tumor tissues and normal ovarian tissues（P>0.05）.The expression of PI3K protein was located in cytoplasm and (or) nucleus.The positive expression rate of PI3K protein in ovarian cancer was significantly higher than those in normal ovary and benign ovarian tumor (P<0.01); there was correlation between the expression intensity of PI3K protein and pathological grading and clinical stages,the worse differentiation of ovarian cancer,the stronger positive expression of PI3K protein（P<0.05）;the higher clinical stage of ovary cancer,the stronger positive expression of PI3K protein（P<0.05）. Conclusion The expression levels of PI3K mRNA and protein in ovarian cancer tissues are significantly increased and PI3K has the role in the occurrence and development of ovarian cancer.

Key words: phosphoinositide 3-kinase, reverse transcriptasepolymerase chain reaction, immunohistochemistry.